Amino acid dipepetide frequency for Prevotella sp. AM42-24

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.45AlaAla: 5.45 ± 0.106
0.939AlaCys: 0.939 ± 0.032
4.224AlaAsp: 4.224 ± 0.066
4.624AlaGlu: 4.624 ± 0.081
3.18AlaPhe: 3.18 ± 0.06
4.564AlaGly: 4.564 ± 0.087
1.313AlaHis: 1.313 ± 0.04
4.922AlaIle: 4.922 ± 0.071
5.247AlaLys: 5.247 ± 0.082
6.271AlaLeu: 6.271 ± 0.081
2.099AlaMet: 2.099 ± 0.049
3.556AlaAsn: 3.556 ± 0.065
2.13AlaPro: 2.13 ± 0.045
2.937AlaGln: 2.937 ± 0.063
2.716AlaArg: 2.716 ± 0.055
4.449AlaSer: 4.449 ± 0.07
3.944AlaThr: 3.944 ± 0.07
4.213AlaVal: 4.213 ± 0.065
0.826AlaTrp: 0.826 ± 0.027
2.954AlaTyr: 2.954 ± 0.051
0.0AlaXaa: 0.0 ± 0.0
Cys
0.784CysAla: 0.784 ± 0.026
0.233CysCys: 0.233 ± 0.017
0.769CysAsp: 0.769 ± 0.025
0.751CysGlu: 0.751 ± 0.028
0.645CysPhe: 0.645 ± 0.026
1.08CysGly: 1.08 ± 0.036
0.317CysHis: 0.317 ± 0.016
0.923CysIle: 0.923 ± 0.034
0.883CysLys: 0.883 ± 0.031
1.133CysLeu: 1.133 ± 0.034
0.371CysMet: 0.371 ± 0.018
0.662CysAsn: 0.662 ± 0.026
0.501CysPro: 0.501 ± 0.025
0.42CysGln: 0.42 ± 0.022
0.572CysArg: 0.572 ± 0.024
0.869CysSer: 0.869 ± 0.029
0.664CysThr: 0.664 ± 0.024
0.782CysVal: 0.782 ± 0.03
0.193CysTrp: 0.193 ± 0.014
0.606CysTyr: 0.606 ± 0.026
0.0CysXaa: 0.0 ± 0.0
Asp
4.336AspAla: 4.336 ± 0.067
0.781AspCys: 0.781 ± 0.028
3.186AspAsp: 3.186 ± 0.067
4.292AspGlu: 4.292 ± 0.075
3.2AspPhe: 3.2 ± 0.058
4.483AspGly: 4.483 ± 0.082
1.066AspHis: 1.066 ± 0.035
4.186AspIle: 4.186 ± 0.072
4.445AspLys: 4.445 ± 0.062
4.509AspLeu: 4.509 ± 0.055
1.752AspMet: 1.752 ± 0.044
2.999AspAsn: 2.999 ± 0.055
1.72AspPro: 1.72 ± 0.041
1.478AspGln: 1.478 ± 0.041
2.384AspArg: 2.384 ± 0.05
3.265AspSer: 3.265 ± 0.065
2.704AspThr: 2.704 ± 0.049
3.838AspVal: 3.838 ± 0.069
0.878AspTrp: 0.878 ± 0.032
3.053AspTyr: 3.053 ± 0.051
0.0AspXaa: 0.0 ± 0.0
Glu
4.521GluAla: 4.521 ± 0.076
0.699GluCys: 0.699 ± 0.026
3.612GluAsp: 3.612 ± 0.052
5.008GluGlu: 5.008 ± 0.1
2.387GluPhe: 2.387 ± 0.051
4.156GluGly: 4.156 ± 0.07
1.316GluHis: 1.316 ± 0.04
4.343GluIle: 4.343 ± 0.062
5.514GluLys: 5.514 ± 0.082
5.367GluLeu: 5.367 ± 0.081
2.08GluMet: 2.08 ± 0.045
3.66GluAsn: 3.66 ± 0.058
1.658GluPro: 1.658 ± 0.04
2.636GluGln: 2.636 ± 0.055
2.954GluArg: 2.954 ± 0.064
3.12GluSer: 3.12 ± 0.055
3.218GluThr: 3.218 ± 0.051
4.007GluVal: 4.007 ± 0.068
0.834GluTrp: 0.834 ± 0.029
2.829GluTyr: 2.829 ± 0.049
0.0GluXaa: 0.0 ± 0.0
Phe
3.176PheAla: 3.176 ± 0.061
0.776PheCys: 0.776 ± 0.027
2.917PheAsp: 2.917 ± 0.05
2.477PheGlu: 2.477 ± 0.048
2.144PhePhe: 2.144 ± 0.053
3.322PheGly: 3.322 ± 0.058
0.933PheHis: 0.933 ± 0.031
2.917PheIle: 2.917 ± 0.061
2.663PheLys: 2.663 ± 0.055
3.842PheLeu: 3.842 ± 0.063
1.207PheMet: 1.207 ± 0.035
2.365PheAsn: 2.365 ± 0.043
1.521PhePro: 1.521 ± 0.037
1.281PheGln: 1.281 ± 0.031
1.92PheArg: 1.92 ± 0.04
3.347PheSer: 3.347 ± 0.073
2.707PheThr: 2.707 ± 0.05
2.853PheVal: 2.853 ± 0.052
0.511PheTrp: 0.511 ± 0.022
2.009PheTyr: 2.009 ± 0.045
0.0PheXaa: 0.0 ± 0.0
Gly
4.319GlyAla: 4.319 ± 0.068
0.965GlyCys: 0.965 ± 0.031
3.657GlyAsp: 3.657 ± 0.063
4.123GlyGlu: 4.123 ± 0.072
3.097GlyPhe: 3.097 ± 0.055
4.67GlyGly: 4.67 ± 0.092
1.27GlyHis: 1.27 ± 0.031
5.196GlyIle: 5.196 ± 0.092
5.973GlyLys: 5.973 ± 0.087
5.359GlyLeu: 5.359 ± 0.081
2.164GlyMet: 2.164 ± 0.045
3.862GlyAsn: 3.862 ± 0.065
1.165GlyPro: 1.165 ± 0.037
2.015GlyGln: 2.015 ± 0.043
2.605GlyArg: 2.605 ± 0.061
4.033GlySer: 4.033 ± 0.086
3.919GlyThr: 3.919 ± 0.069
4.555GlyVal: 4.555 ± 0.072
1.039GlyTrp: 1.039 ± 0.032
3.313GlyTyr: 3.313 ± 0.061
0.0GlyXaa: 0.0 ± 0.0
His
1.175HisAla: 1.175 ± 0.037
0.335HisCys: 0.335 ± 0.019
1.172HisAsp: 1.172 ± 0.035
1.131HisGlu: 1.131 ± 0.032
1.139HisPhe: 1.139 ± 0.034
1.359HisGly: 1.359 ± 0.035
0.635HisHis: 0.635 ± 0.03
1.457HisIle: 1.457 ± 0.043
1.165HisLys: 1.165 ± 0.035
1.856HisLeu: 1.856 ± 0.047
0.347HisMet: 0.347 ± 0.016
1.054HisAsn: 1.054 ± 0.031
0.963HisPro: 0.963 ± 0.031
0.803HisGln: 0.803 ± 0.028
0.946HisArg: 0.946 ± 0.03
1.219HisSer: 1.219 ± 0.035
1.077HisThr: 1.077 ± 0.027
1.251HisVal: 1.251 ± 0.035
0.254HisTrp: 0.254 ± 0.017
0.985HisTyr: 0.985 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
5.02IleAla: 5.02 ± 0.084
1.029IleCys: 1.029 ± 0.034
4.589IleAsp: 4.589 ± 0.07
4.323IleGlu: 4.323 ± 0.063
2.852IlePhe: 2.852 ± 0.065
4.501IleGly: 4.501 ± 0.077
1.328IleHis: 1.328 ± 0.037
4.63IleIle: 4.63 ± 0.091
4.59IleLys: 4.59 ± 0.074
5.524IleLeu: 5.524 ± 0.083
1.602IleMet: 1.602 ± 0.04
3.504IleAsn: 3.504 ± 0.064
2.966IlePro: 2.966 ± 0.052
2.244IleGln: 2.244 ± 0.044
3.016IleArg: 3.016 ± 0.057
4.592IleSer: 4.592 ± 0.065
4.006IleThr: 4.006 ± 0.071
4.178IleVal: 4.178 ± 0.069
0.712IleTrp: 0.712 ± 0.022
2.794IleTyr: 2.794 ± 0.051
0.0IleXaa: 0.0 ± 0.0
Lys
5.487LysAla: 5.487 ± 0.088
0.659LysCys: 0.659 ± 0.027
4.562LysAsp: 4.562 ± 0.064
5.77LysGlu: 5.77 ± 0.082
2.656LysPhe: 2.656 ± 0.046
4.674LysGly: 4.674 ± 0.061
1.387LysHis: 1.387 ± 0.031
4.521LysIle: 4.521 ± 0.073
5.973LysLys: 5.973 ± 0.092
5.712LysLeu: 5.712 ± 0.071
2.661LysMet: 2.661 ± 0.05
4.231LysAsn: 4.231 ± 0.071
2.275LysPro: 2.275 ± 0.049
2.951LysGln: 2.951 ± 0.05
3.091LysArg: 3.091 ± 0.059
3.917LysSer: 3.917 ± 0.061
4.067LysThr: 4.067 ± 0.055
4.708LysVal: 4.708 ± 0.067
0.924LysTrp: 0.924 ± 0.03
3.416LysTyr: 3.416 ± 0.057
0.0LysXaa: 0.0 ± 0.0
Leu
6.171LeuAla: 6.171 ± 0.09
1.278LeuCys: 1.278 ± 0.034
4.81LeuAsp: 4.81 ± 0.072
4.73LeuGlu: 4.73 ± 0.075
3.914LeuPhe: 3.914 ± 0.073
5.54LeuGly: 5.54 ± 0.08
1.833LeuHis: 1.833 ± 0.043
4.986LeuIle: 4.986 ± 0.085
6.38LeuLys: 6.38 ± 0.074
7.732LeuLeu: 7.732 ± 0.11
2.588LeuMet: 2.588 ± 0.054
4.48LeuAsn: 4.48 ± 0.071
3.603LeuPro: 3.603 ± 0.064
3.396LeuGln: 3.396 ± 0.061
3.853LeuArg: 3.853 ± 0.062
6.257LeuSer: 6.257 ± 0.083
4.925LeuThr: 4.925 ± 0.062
5.068LeuVal: 5.068 ± 0.073
0.967LeuTrp: 0.967 ± 0.032
3.475LeuTyr: 3.475 ± 0.068
0.001LeuXaa: 0.001 ± 0.001
Met
2.423MetAla: 2.423 ± 0.046
0.264MetCys: 0.264 ± 0.015
1.631MetAsp: 1.631 ± 0.041
1.868MetGlu: 1.868 ± 0.045
1.067MetPhe: 1.067 ± 0.035
1.975MetGly: 1.975 ± 0.044
0.538MetHis: 0.538 ± 0.025
1.714MetIle: 1.714 ± 0.045
2.727MetLys: 2.727 ± 0.05
2.624MetLeu: 2.624 ± 0.048
1.093MetMet: 1.093 ± 0.034
1.651MetAsn: 1.651 ± 0.04
1.176MetPro: 1.176 ± 0.03
1.169MetGln: 1.169 ± 0.036
1.334MetArg: 1.334 ± 0.035
1.734MetSer: 1.734 ± 0.045
1.647MetThr: 1.647 ± 0.036
1.818MetVal: 1.818 ± 0.042
0.258MetTrp: 0.258 ± 0.017
0.841MetTyr: 0.841 ± 0.025
0.0MetXaa: 0.0 ± 0.0
Asn
3.721AsnAla: 3.721 ± 0.064
0.592AsnCys: 0.592 ± 0.024
2.907AsnAsp: 2.907 ± 0.06
3.127AsnGlu: 3.127 ± 0.049
2.326AsnPhe: 2.326 ± 0.048
4.162AsnGly: 4.162 ± 0.08
1.19AsnHis: 1.19 ± 0.033
3.999AsnIle: 3.999 ± 0.068
3.519AsnLys: 3.519 ± 0.053
4.522AsnLeu: 4.522 ± 0.078
1.451AsnMet: 1.451 ± 0.038
2.924AsnAsn: 2.924 ± 0.056
2.492AsnPro: 2.492 ± 0.059
1.876AsnGln: 1.876 ± 0.041
2.321AsnArg: 2.321 ± 0.047
3.06AsnSer: 3.06 ± 0.056
2.76AsnThr: 2.76 ± 0.053
3.436AsnVal: 3.436 ± 0.064
0.731AsnTrp: 0.731 ± 0.029
2.5AsnTyr: 2.5 ± 0.051
0.0AsnXaa: 0.0 ± 0.0
Pro
2.38ProAla: 2.38 ± 0.046
0.386ProCys: 0.386 ± 0.02
2.226ProAsp: 2.226 ± 0.049
2.743ProGlu: 2.743 ± 0.05
1.67ProPhe: 1.67 ± 0.039
2.059ProGly: 2.059 ± 0.045
0.729ProHis: 0.729 ± 0.023
2.264ProIle: 2.264 ± 0.045
2.163ProLys: 2.163 ± 0.048
2.898ProLeu: 2.898 ± 0.056
0.985ProMet: 0.985 ± 0.033
1.727ProAsn: 1.727 ± 0.035
0.654ProPro: 0.654 ± 0.024
1.361ProGln: 1.361 ± 0.037
1.14ProArg: 1.14 ± 0.037
2.193ProSer: 2.193 ± 0.049
2.062ProThr: 2.062 ± 0.049
2.551ProVal: 2.551 ± 0.049
0.441ProTrp: 0.441 ± 0.022
1.655ProTyr: 1.655 ± 0.041
0.001ProXaa: 0.001 ± 0.001
Gln
2.629GlnAla: 2.629 ± 0.054
0.376GlnCys: 0.376 ± 0.019
1.771GlnAsp: 1.771 ± 0.038
2.431GlnGlu: 2.431 ± 0.061
1.373GlnPhe: 1.373 ± 0.037
2.157GlnGly: 2.157 ± 0.038
0.73GlnHis: 0.73 ± 0.026
2.43GlnIle: 2.43 ± 0.052
2.918GlnLys: 2.918 ± 0.059
3.404GlnLeu: 3.404 ± 0.056
1.224GlnMet: 1.224 ± 0.033
1.981GlnAsn: 1.981 ± 0.041
1.279GlnPro: 1.279 ± 0.03
1.761GlnGln: 1.761 ± 0.048
1.659GlnArg: 1.659 ± 0.045
1.892GlnSer: 1.892 ± 0.039
2.041GlnThr: 2.041 ± 0.042
2.289GlnVal: 2.289 ± 0.046
0.475GlnTrp: 0.475 ± 0.019
1.587GlnTyr: 1.587 ± 0.034
0.0GlnXaa: 0.0 ± 0.0
Arg
2.514ArgAla: 2.514 ± 0.057
0.505ArgCys: 0.505 ± 0.024
2.176ArgAsp: 2.176 ± 0.052
2.614ArgGlu: 2.614 ± 0.063
2.069ArgPhe: 2.069 ± 0.043
2.356ArgGly: 2.356 ± 0.053
0.939ArgHis: 0.939 ± 0.027
3.254ArgIle: 3.254 ± 0.061
3.459ArgLys: 3.459 ± 0.063
3.933ArgLeu: 3.933 ± 0.064
1.504ArgMet: 1.504 ± 0.036
2.439ArgAsn: 2.439 ± 0.055
1.422ArgPro: 1.422 ± 0.036
1.884ArgGln: 1.884 ± 0.041
2.018ArgArg: 2.018 ± 0.051
2.139ArgSer: 2.139 ± 0.045
2.099ArgThr: 2.099 ± 0.045
2.394ArgVal: 2.394 ± 0.049
0.58ArgTrp: 0.58 ± 0.023
2.049ArgTyr: 2.049 ± 0.046
0.0ArgXaa: 0.0 ± 0.0
Ser
4.279SerAla: 4.279 ± 0.073
0.92SerCys: 0.92 ± 0.031
3.585SerAsp: 3.585 ± 0.067
3.507SerGlu: 3.507 ± 0.061
3.165SerPhe: 3.165 ± 0.053
4.118SerGly: 4.118 ± 0.073
1.268SerHis: 1.268 ± 0.038
4.432SerIle: 4.432 ± 0.077
4.041SerLys: 4.041 ± 0.068
5.853SerLeu: 5.853 ± 0.078
1.654SerMet: 1.654 ± 0.042
3.098SerAsn: 3.098 ± 0.063
2.046SerPro: 2.046 ± 0.039
2.205SerGln: 2.205 ± 0.05
2.404SerArg: 2.404 ± 0.048
4.057SerSer: 4.057 ± 0.074
3.225SerThr: 3.225 ± 0.063
4.017SerVal: 4.017 ± 0.058
0.805SerTrp: 0.805 ± 0.03
2.926SerTyr: 2.926 ± 0.061
0.0SerXaa: 0.0 ± 0.0
Thr
3.939ThrAla: 3.939 ± 0.067
0.617ThrCys: 0.617 ± 0.024
3.351ThrAsp: 3.351 ± 0.059
3.211ThrGlu: 3.211 ± 0.059
2.654ThrPhe: 2.654 ± 0.058
3.984ThrGly: 3.984 ± 0.073
1.087ThrHis: 1.087 ± 0.036
4.03ThrIle: 4.03 ± 0.06
3.258ThrLys: 3.258 ± 0.056
5.079ThrLeu: 5.079 ± 0.067
1.451ThrMet: 1.451 ± 0.031
2.577ThrAsn: 2.577 ± 0.061
2.467ThrPro: 2.467 ± 0.051
1.779ThrGln: 1.779 ± 0.043
2.041ThrArg: 2.041 ± 0.052
3.53ThrSer: 3.53 ± 0.07
3.272ThrThr: 3.272 ± 0.071
3.586ThrVal: 3.586 ± 0.06
0.662ThrTrp: 0.662 ± 0.028
2.544ThrTyr: 2.544 ± 0.06
0.0ThrXaa: 0.0 ± 0.0
Val
4.504ValAla: 4.504 ± 0.073
0.982ValCys: 0.982 ± 0.031
3.826ValAsp: 3.826 ± 0.059
4.034ValGlu: 4.034 ± 0.064
2.757ValPhe: 2.757 ± 0.047
4.133ValGly: 4.133 ± 0.07
1.051ValHis: 1.051 ± 0.028
4.217ValIle: 4.217 ± 0.062
4.818ValLys: 4.818 ± 0.064
5.326ValLeu: 5.326 ± 0.077
1.75ValMet: 1.75 ± 0.043
3.374ValAsn: 3.374 ± 0.052
2.282ValPro: 2.282 ± 0.045
1.838ValGln: 1.838 ± 0.039
2.658ValArg: 2.658 ± 0.048
4.385ValSer: 4.385 ± 0.073
3.599ValThr: 3.599 ± 0.069
4.427ValVal: 4.427 ± 0.068
0.715ValTrp: 0.715 ± 0.029
2.56ValTyr: 2.56 ± 0.04
0.0ValXaa: 0.0 ± 0.0
Trp
0.799TrpAla: 0.799 ± 0.029
0.174TrpCys: 0.174 ± 0.012
0.704TrpAsp: 0.704 ± 0.027
0.684TrpGlu: 0.684 ± 0.022
0.556TrpPhe: 0.556 ± 0.025
0.925TrpGly: 0.925 ± 0.031
0.303TrpHis: 0.303 ± 0.016
0.774TrpIle: 0.774 ± 0.028
1.036TrpLys: 1.036 ± 0.039
1.157TrpLeu: 1.157 ± 0.04
0.461TrpMet: 0.461 ± 0.021
0.884TrpAsn: 0.884 ± 0.029
0.259TrpPro: 0.259 ± 0.015
0.55TrpGln: 0.55 ± 0.025
0.559TrpArg: 0.559 ± 0.022
0.704TrpSer: 0.704 ± 0.027
0.706TrpThr: 0.706 ± 0.026
0.673TrpVal: 0.673 ± 0.025
0.181TrpTrp: 0.181 ± 0.015
0.502TrpTyr: 0.502 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.93TyrAla: 2.93 ± 0.056
0.651TyrCys: 0.651 ± 0.027
2.877TyrAsp: 2.877 ± 0.063
2.467TyrGlu: 2.467 ± 0.046
2.04TyrPhe: 2.04 ± 0.041
3.083TyrGly: 3.083 ± 0.068
1.09TyrHis: 1.09 ± 0.031
2.805TyrIle: 2.805 ± 0.057
2.869TyrLys: 2.869 ± 0.05
3.885TyrLeu: 3.885 ± 0.071
1.109TyrMet: 1.109 ± 0.03
2.587TyrAsn: 2.587 ± 0.056
1.709TyrPro: 1.709 ± 0.037
1.822TyrGln: 1.822 ± 0.038
2.153TyrArg: 2.153 ± 0.046
2.846TyrSer: 2.846 ± 0.059
2.478TyrThr: 2.478 ± 0.055
2.593TyrVal: 2.593 ± 0.045
0.604TyrTrp: 0.604 ± 0.027
2.307TyrTyr: 2.307 ± 0.058
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.001XaaArg: 0.001 ± 0.001
0.001XaaSer: 0.001 ± 0.001
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.004XaaXaa: 0.004 ± 0.002
Statistics based on 3181 proteins (1115435 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski