Johnsongrass chlorotic stripe mosaic virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Aureusvirus

Average proteome isoelectric point is 8.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q70P83|Q70P83_9TOMB Putative movement protein OS=Johnsongrass chlorotic stripe mosaic virus OX=229149 GN=p24 PE=3 SV=1
MM1 pKa = 7.89EE2 pKa = 5.32GPKK5 pKa = 10.38DD6 pKa = 3.68WVLHH10 pKa = 4.52PHH12 pKa = 6.61RR13 pKa = 11.84CDD15 pKa = 3.35FCGHH19 pKa = 5.66ATYY22 pKa = 10.65LRR24 pKa = 11.84EE25 pKa = 4.24CWRR28 pKa = 11.84HH29 pKa = 6.15GSDD32 pKa = 3.0QVNRR36 pKa = 11.84HH37 pKa = 5.33EE38 pKa = 4.19RR39 pKa = 11.84HH40 pKa = 5.57EE41 pKa = 4.18PFPRR45 pKa = 11.84LVQVQGVQPGTWPDD59 pKa = 2.84IGRR62 pKa = 11.84VTTAVLPANRR72 pKa = 11.84GVSYY76 pKa = 8.27TLRR79 pKa = 11.84GHH81 pKa = 6.6GVTITVSGHH90 pKa = 5.4EE91 pKa = 3.57NDD93 pKa = 3.85VFNVARR99 pKa = 11.84VASDD103 pKa = 3.46VLTHH107 pKa = 6.3PVIQGEE113 pKa = 4.22ICVRR117 pKa = 11.84GSPPTGVGGGNQLPYY132 pKa = 10.7EE133 pKa = 4.33NQTNIVV139 pKa = 3.37

Molecular weight: 15.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q70P86|Q70P86_9TOMB RNA-directed RNA polymerase OS=Johnsongrass chlorotic stripe mosaic virus OX=229149 PE=3 SV=1
MM1 pKa = 7.76DD2 pKa = 3.95TGILSRR8 pKa = 11.84RR9 pKa = 11.84IVTAEE14 pKa = 3.44VDD16 pKa = 4.13FQFGSVDD23 pKa = 3.38YY24 pKa = 9.92SDD26 pKa = 4.02PRR28 pKa = 11.84IVHH31 pKa = 6.6ALCTPGLKK39 pKa = 9.78EE40 pKa = 3.37RR41 pKa = 11.84ATFGRR46 pKa = 11.84QIVTALKK53 pKa = 9.06MAVIALTLPVWWPLRR68 pKa = 11.84LVWRR72 pKa = 11.84VIIMGVLWVTRR83 pKa = 11.84FXTRR87 pKa = 11.84CTNLIKK93 pKa = 10.2WCVKK97 pKa = 5.9EE98 pKa = 4.02TRR100 pKa = 11.84VTVRR104 pKa = 11.84AYY106 pKa = 9.71WNILNKK112 pKa = 9.85RR113 pKa = 11.84ARR115 pKa = 11.84GLVVLGCWASFVLYY129 pKa = 10.54GPYY132 pKa = 10.87ALLLWLGVIVGYY144 pKa = 9.44IICVLPSNVRR154 pKa = 11.84YY155 pKa = 10.07YY156 pKa = 10.25IEE158 pKa = 5.12LGQKK162 pKa = 9.0IQDD165 pKa = 3.11AWDD168 pKa = 3.52SVEE171 pKa = 4.85ADD173 pKa = 3.76DD174 pKa = 6.08TIEE177 pKa = 4.29APCNGDD183 pKa = 2.97ILEE186 pKa = 4.17VRR188 pKa = 11.84KK189 pKa = 10.19GRR191 pKa = 11.84NKK193 pKa = 9.85FACKK197 pKa = 9.87LAARR201 pKa = 11.84AIGRR205 pKa = 11.84VGLLKK210 pKa = 9.95ATPANALVYY219 pKa = 10.31QKK221 pKa = 11.18VILDD225 pKa = 3.4EE226 pKa = 4.15MKK228 pKa = 10.24ILNVRR233 pKa = 11.84FADD236 pKa = 3.85RR237 pKa = 11.84VRR239 pKa = 11.84ILPLAVMASLDD250 pKa = 3.71RR251 pKa = 11.84PDD253 pKa = 3.31AVARR257 pKa = 11.84VEE259 pKa = 4.26DD260 pKa = 4.32CVAALTQRR268 pKa = 11.84GVSLL272 pKa = 3.89

Molecular weight: 30.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1787

139

795

357.4

39.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.051 ± 0.916

2.462 ± 0.248

4.701 ± 0.564

4.141 ± 0.456

3.973 ± 0.539

8.45 ± 0.997

1.847 ± 0.657

4.253 ± 0.658

4.365 ± 0.829

9.01 ± 0.741

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.071 ± 0.257

3.414 ± 0.69

5.204 ± 0.578

3.134 ± 0.482

7.051 ± 0.702

5.82 ± 0.931

6.715 ± 0.748

10.688 ± 0.651

2.071 ± 0.323

3.358 ± 0.337
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski