Nocardioides sp. dk884

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides; unclassified Nocardioides

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3911 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q0NWX6|A0A5Q0NWX6_9ACTN Histidine kinase OS=Nocardioides sp. dk884 OX=2662361 GN=GFH29_12940 PE=4 SV=1
MM1 pKa = 7.74TIHH4 pKa = 6.85LHH6 pKa = 5.83RR7 pKa = 11.84VAALGAALILGAGSFAACGANDD29 pKa = 4.4DD30 pKa = 5.17NSDD33 pKa = 3.53NDD35 pKa = 3.94TGDD38 pKa = 3.43GGGGGGDD45 pKa = 3.5SASGGTIALLLPEE58 pKa = 4.87SKK60 pKa = 7.49TTRR63 pKa = 11.84YY64 pKa = 9.33EE65 pKa = 4.29AFDD68 pKa = 3.81KK69 pKa = 10.97PLFEE73 pKa = 5.11AKK75 pKa = 9.97VAEE78 pKa = 4.32LCPDD82 pKa = 3.91CEE84 pKa = 3.85VDD86 pKa = 5.47YY87 pKa = 11.79YY88 pKa = 11.64NADD91 pKa = 3.31QDD93 pKa = 4.02EE94 pKa = 4.48QKK96 pKa = 10.32QAQQVTSALTAGADD110 pKa = 3.67VMVLDD115 pKa = 4.85PVNGDD120 pKa = 3.2GATGMVQEE128 pKa = 4.86AQGQDD133 pKa = 3.61VPVIAYY139 pKa = 9.85DD140 pKa = 3.76RR141 pKa = 11.84FIEE144 pKa = 4.56GADD147 pKa = 3.48YY148 pKa = 11.62YY149 pKa = 10.99MSFDD153 pKa = 3.72NQTVGQMQAEE163 pKa = 4.23ALVEE167 pKa = 4.08AVGDD171 pKa = 3.71TGSILMLNGDD181 pKa = 4.45PKK183 pKa = 11.19DD184 pKa = 3.71PNAGQFKK191 pKa = 10.57AGAHH195 pKa = 5.48SVLDD199 pKa = 3.92EE200 pKa = 4.62SGVEE204 pKa = 4.06VLAEE208 pKa = 3.99YY209 pKa = 10.6DD210 pKa = 3.69NPDD213 pKa = 3.38WSPEE217 pKa = 3.82NAQQWTTDD225 pKa = 3.44QLSKK229 pKa = 10.98FEE231 pKa = 4.24PSEE234 pKa = 3.88IVGVYY239 pKa = 9.49AANDD243 pKa = 3.92GQAGGVVAAMTGAGVARR260 pKa = 11.84NQLPPITGQDD270 pKa = 3.26AEE272 pKa = 4.41VAAIQRR278 pKa = 11.84ILAGEE283 pKa = 3.94QAMTIYY289 pKa = 10.59KK290 pKa = 9.74PIAIEE295 pKa = 4.19AEE297 pKa = 4.12TAAEE301 pKa = 4.22VAVQLANGEE310 pKa = 4.43EE311 pKa = 4.21PGATSDD317 pKa = 3.76TGIDD321 pKa = 3.28QSDD324 pKa = 3.93FEE326 pKa = 5.19GVTSYY331 pKa = 10.9IFDD334 pKa = 5.53PIVVTSDD341 pKa = 2.93NVADD345 pKa = 3.91TVIADD350 pKa = 4.2GFYY353 pKa = 9.81TAEE356 pKa = 4.99DD357 pKa = 3.82ICTGDD362 pKa = 3.51YY363 pKa = 11.36AKK365 pKa = 10.87ACAEE369 pKa = 4.13AGISS373 pKa = 3.45

Molecular weight: 38.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q0NQD3|A0A5Q0NQD3_9ACTN TetR family transcriptional regulator OS=Nocardioides sp. dk884 OX=2662361 GN=GFH29_03880 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.24TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.07LGKK33 pKa = 9.87

Molecular weight: 4.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3911

0

3911

1273211

29

2092

325.5

34.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.536 ± 0.053

0.737 ± 0.012

6.278 ± 0.028

6.071 ± 0.038

2.665 ± 0.02

9.247 ± 0.039

2.27 ± 0.024

3.263 ± 0.026

1.766 ± 0.026

10.441 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.794 ± 0.015

1.602 ± 0.02

5.756 ± 0.031

2.666 ± 0.018

8.015 ± 0.042

5.076 ± 0.025

5.958 ± 0.034

9.469 ± 0.034

1.512 ± 0.015

1.879 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski