Amino acid dipepetide frequency for Oxobacter pfennigii

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.656AlaAla: 6.656 ± 0.105	0.861AlaCys: 0.861 ± 0.028	3.659AlaAsp: 3.659 ± 0.056	4.426AlaGlu: 4.426 ± 0.063	2.951AlaPhe: 2.951 ± 0.052	5.567AlaGly: 5.567 ± 0.082	0.974AlaHis: 0.974 ± 0.03	5.727AlaIle: 5.727 ± 0.062	4.599AlaLys: 4.599 ± 0.068	6.894AlaLeu: 6.894 ± 0.097	2.053AlaMet: 2.053 ± 0.045	2.519AlaAsn: 2.519 ± 0.052	1.989AlaPro: 1.989 ± 0.048	1.974AlaGln: 1.974 ± 0.037	2.532AlaArg: 2.532 ± 0.044	4.01AlaSer: 4.01 ± 0.064	2.741AlaThr: 2.741 ± 0.083	5.738AlaVal: 5.738 ± 0.08	0.495AlaTrp: 0.495 ± 0.022	2.46AlaTyr: 2.46 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	0.758CysAla: 0.758 ± 0.027	0.224CysCys: 0.224 ± 0.014	0.706CysAsp: 0.706 ± 0.027	0.658CysGlu: 0.658 ± 0.023	0.501CysPhe: 0.501 ± 0.021	1.199CysGly: 1.199 ± 0.033	0.233CysHis: 0.233 ± 0.015	1.059CysIle: 1.059 ± 0.029	0.724CysLys: 0.724 ± 0.025	0.906CysLeu: 0.906 ± 0.027	0.325CysMet: 0.325 ± 0.018	0.493CysAsn: 0.493 ± 0.022	0.539CysPro: 0.539 ± 0.025	0.303CysGln: 0.303 ± 0.014	0.538CysArg: 0.538 ± 0.022	0.792CysSer: 0.792 ± 0.025	0.609CysThr: 0.609 ± 0.023	0.684CysVal: 0.684 ± 0.029	0.084CysTrp: 0.084 ± 0.008	0.408CysTyr: 0.408 ± 0.022	0.0CysXaa: 0.0 ± 0.0
Asp	3.502AspAla: 3.502 ± 0.068	0.657AspCys: 0.657 ± 0.025	2.885AspAsp: 2.885 ± 0.055	4.567AspGlu: 4.567 ± 0.067	2.661AspPhe: 2.661 ± 0.048	3.555AspGly: 3.555 ± 0.066	0.64AspHis: 0.64 ± 0.022	6.398AspIle: 6.398 ± 0.081	4.675AspLys: 4.675 ± 0.068	4.439AspLeu: 4.439 ± 0.062	1.758AspMet: 1.758 ± 0.039	2.76AspAsn: 2.76 ± 0.045	1.652AspPro: 1.652 ± 0.035	0.832AspGln: 0.832 ± 0.028	2.244AspArg: 2.244 ± 0.049	3.114AspSer: 3.114 ± 0.055	2.93AspThr: 2.93 ± 0.061	3.698AspVal: 3.698 ± 0.055	0.537AspTrp: 0.537 ± 0.022	2.684AspTyr: 2.684 ± 0.049	0.0AspXaa: 0.0 ± 0.0
Glu	4.857GluAla: 4.857 ± 0.076	0.585GluCys: 0.585 ± 0.024	3.981GluAsp: 3.981 ± 0.062	5.624GluGlu: 5.624 ± 0.084	2.762GluPhe: 2.762 ± 0.043	4.395GluGly: 4.395 ± 0.053	1.09GluHis: 1.09 ± 0.029	6.25GluIle: 6.25 ± 0.077	6.341GluLys: 6.341 ± 0.084	6.402GluLeu: 6.402 ± 0.065	1.944GluMet: 1.944 ± 0.04	4.089GluAsn: 4.089 ± 0.05	1.72GluPro: 1.72 ± 0.046	2.059GluGln: 2.059 ± 0.047	2.956GluArg: 2.956 ± 0.053	3.291GluSer: 3.291 ± 0.055	3.029GluThr: 3.029 ± 0.052	4.396GluVal: 4.396 ± 0.064	0.555GluTrp: 0.555 ± 0.022	2.983GluTyr: 2.983 ± 0.046	0.0GluXaa: 0.0 ± 0.0
Phe	2.735PheAla: 2.735 ± 0.052	0.552PheCys: 0.552 ± 0.021	2.573PheAsp: 2.573 ± 0.044	2.835PheGlu: 2.835 ± 0.055	1.952PhePhe: 1.952 ± 0.052	2.896PheGly: 2.896 ± 0.053	0.645PheHis: 0.645 ± 0.023	4.153PheIle: 4.153 ± 0.068	3.084PheLys: 3.084 ± 0.051	3.992PheLeu: 3.992 ± 0.065	1.341PheMet: 1.341 ± 0.032	2.312PheAsn: 2.312 ± 0.043	1.389PhePro: 1.389 ± 0.034	1.015PheGln: 1.015 ± 0.026	1.254PheArg: 1.254 ± 0.035	3.109PheSer: 3.109 ± 0.051	2.428PheThr: 2.428 ± 0.048	2.54PheVal: 2.54 ± 0.043	0.368PheTrp: 0.368 ± 0.018	1.843PheTyr: 1.843 ± 0.043	0.0PheXaa: 0.0 ± 0.0
Gly	4.784GlyAla: 4.784 ± 0.091	0.979GlyCys: 0.979 ± 0.029	3.512GlyAsp: 3.512 ± 0.063	4.299GlyGlu: 4.299 ± 0.064	3.294GlyPhe: 3.294 ± 0.051	4.964GlyGly: 4.964 ± 0.077	1.139GlyHis: 1.139 ± 0.033	6.821GlyIle: 6.821 ± 0.083	5.281GlyLys: 5.281 ± 0.065	5.982GlyLeu: 5.982 ± 0.077	2.198GlyMet: 2.198 ± 0.043	3.174GlyAsn: 3.174 ± 0.066	1.54GlyPro: 1.54 ± 0.046	1.943GlyGln: 1.943 ± 0.038	2.879GlyArg: 2.879 ± 0.054	4.339GlySer: 4.339 ± 0.08	3.906GlyThr: 3.906 ± 0.076	4.545GlyVal: 4.545 ± 0.068	0.692GlyTrp: 0.692 ± 0.028	3.266GlyTyr: 3.266 ± 0.055	0.0GlyXaa: 0.0 ± 0.0
His	0.876HisAla: 0.876 ± 0.028	0.202HisCys: 0.202 ± 0.013	0.84HisAsp: 0.84 ± 0.027	0.994HisGlu: 0.994 ± 0.03	0.654HisPhe: 0.654 ± 0.023	1.105HisGly: 1.105 ± 0.028	0.365HisHis: 0.365 ± 0.022	1.431HisIle: 1.431 ± 0.033	1.017HisLys: 1.017 ± 0.031	1.254HisLeu: 1.254 ± 0.034	0.458HisMet: 0.458 ± 0.017	0.76HisAsn: 0.76 ± 0.027	0.754HisPro: 0.754 ± 0.03	0.392HisGln: 0.392 ± 0.018	0.628HisArg: 0.628 ± 0.025	1.024HisSer: 1.024 ± 0.032	0.769HisThr: 0.769 ± 0.027	0.825HisVal: 0.825 ± 0.025	0.149HisTrp: 0.149 ± 0.012	0.638HisTyr: 0.638 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	6.344IleAla: 6.344 ± 0.076	1.091IleCys: 1.091 ± 0.026	5.348IleAsp: 5.348 ± 0.063	6.195IleGlu: 6.195 ± 0.075	3.819IlePhe: 3.819 ± 0.071	5.836IleGly: 5.836 ± 0.089	1.281IleHis: 1.281 ± 0.03	8.358IleIle: 8.358 ± 0.13	7.332IleLys: 7.332 ± 0.087	8.315IleLeu: 8.315 ± 0.086	2.443IleMet: 2.443 ± 0.045	4.995IleAsn: 4.995 ± 0.068	3.435IlePro: 3.435 ± 0.047	2.122IleGln: 2.122 ± 0.042	3.317IleArg: 3.317 ± 0.052	6.476IleSer: 6.476 ± 0.074	4.825IleThr: 4.825 ± 0.075	5.491IleVal: 5.491 ± 0.07	0.606IleTrp: 0.606 ± 0.022	3.347IleTyr: 3.347 ± 0.068	0.0IleXaa: 0.0 ± 0.0
Lys	5.147LysAla: 5.147 ± 0.075	0.729LysCys: 0.729 ± 0.03	4.852LysAsp: 4.852 ± 0.064	6.643LysGlu: 6.643 ± 0.083	2.512LysPhe: 2.512 ± 0.048	4.913LysGly: 4.913 ± 0.08	1.084LysHis: 1.084 ± 0.033	6.607LysIle: 6.607 ± 0.085	6.392LysLys: 6.392 ± 0.087	6.423LysLeu: 6.423 ± 0.083	2.243LysMet: 2.243 ± 0.047	4.711LysAsn: 4.711 ± 0.064	2.345LysPro: 2.345 ± 0.045	1.985LysGln: 1.985 ± 0.042	3.206LysArg: 3.206 ± 0.051	4.574LysSer: 4.574 ± 0.064	3.725LysThr: 3.725 ± 0.06	4.805LysVal: 4.805 ± 0.066	0.596LysTrp: 0.596 ± 0.022	3.378LysTyr: 3.378 ± 0.054	0.0LysXaa: 0.0 ± 0.0
Leu	5.893LeuAla: 5.893 ± 0.079	1.257LeuCys: 1.257 ± 0.035	4.9LeuAsp: 4.9 ± 0.071	5.557LeuGlu: 5.557 ± 0.073	3.926LeuPhe: 3.926 ± 0.072	6.033LeuGly: 6.033 ± 0.084	1.328LeuHis: 1.328 ± 0.03	7.646LeuIle: 7.646 ± 0.101	7.869LeuLys: 7.869 ± 0.092	8.684LeuLeu: 8.684 ± 0.107	2.662LeuMet: 2.662 ± 0.054	5.019LeuAsn: 5.019 ± 0.072	3.446LeuPro: 3.446 ± 0.054	2.225LeuGln: 2.225 ± 0.047	3.563LeuArg: 3.563 ± 0.059	7.164LeuSer: 7.164 ± 0.08	4.836LeuThr: 4.836 ± 0.062	5.081LeuVal: 5.081 ± 0.07	0.731LeuTrp: 0.731 ± 0.025	3.454LeuTyr: 3.454 ± 0.064	0.0LeuXaa: 0.0 ± 0.0
Met	2.233MetAla: 2.233 ± 0.035	0.241MetCys: 0.241 ± 0.014	1.885MetAsp: 1.885 ± 0.041	2.154MetGlu: 2.154 ± 0.048	1.027MetPhe: 1.027 ± 0.031	2.187MetGly: 2.187 ± 0.041	0.386MetHis: 0.386 ± 0.017	2.147MetIle: 2.147 ± 0.042	2.356MetLys: 2.356 ± 0.039	2.809MetLeu: 2.809 ± 0.047	0.813MetMet: 0.813 ± 0.026	1.563MetAsn: 1.563 ± 0.037	1.174MetPro: 1.174 ± 0.031	0.801MetGln: 0.801 ± 0.025	1.125MetArg: 1.125 ± 0.031	1.809MetSer: 1.809 ± 0.038	1.494MetThr: 1.494 ± 0.033	1.873MetVal: 1.873 ± 0.044	0.199MetTrp: 0.199 ± 0.012	0.896MetTyr: 0.896 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	3.226AsnAla: 3.226 ± 0.052	0.597AsnCys: 0.597 ± 0.02	2.498AsnAsp: 2.498 ± 0.046	3.365AsnGlu: 3.365 ± 0.06	2.073AsnPhe: 2.073 ± 0.042	3.188AsnGly: 3.188 ± 0.064	0.752AsnHis: 0.752 ± 0.02	5.418AsnIle: 5.418 ± 0.085	3.934AsnLys: 3.934 ± 0.072	4.524AsnLeu: 4.524 ± 0.05	1.587AsnMet: 1.587 ± 0.033	2.816AsnAsn: 2.816 ± 0.055	2.295AsnPro: 2.295 ± 0.043	1.268AsnGln: 1.268 ± 0.031	1.951AsnArg: 1.951 ± 0.043	3.233AsnSer: 3.233 ± 0.064	2.767AsnThr: 2.767 ± 0.054	3.016AsnVal: 3.016 ± 0.052	0.425AsnTrp: 0.425 ± 0.02	2.13AsnTyr: 2.13 ± 0.051	0.0AsnXaa: 0.0 ± 0.0
Pro	2.291ProAla: 2.291 ± 0.053	0.332ProCys: 0.332 ± 0.016	2.339ProAsp: 2.339 ± 0.053	2.901ProGlu: 2.901 ± 0.055	1.548ProPhe: 1.548 ± 0.034	2.481ProGly: 2.481 ± 0.047	0.603ProHis: 0.603 ± 0.022	2.574ProIle: 2.574 ± 0.051	2.049ProLys: 2.049 ± 0.04	2.915ProLeu: 2.915 ± 0.052	0.883ProMet: 0.883 ± 0.028	1.306ProAsn: 1.306 ± 0.034	0.999ProPro: 0.999 ± 0.033	1.129ProGln: 1.129 ± 0.029	1.076ProArg: 1.076 ± 0.03	2.042ProSer: 2.042 ± 0.038	1.553ProThr: 1.553 ± 0.046	2.946ProVal: 2.946 ± 0.052	0.286ProTrp: 0.286 ± 0.016	1.447ProTyr: 1.447 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	1.855GlnAla: 1.855 ± 0.045	0.267GlnCys: 0.267 ± 0.017	1.377GlnAsp: 1.377 ± 0.03	1.812GlnGlu: 1.812 ± 0.044	1.057GlnPhe: 1.057 ± 0.029	1.706GlnGly: 1.706 ± 0.039	0.396GlnHis: 0.396 ± 0.017	2.08GlnIle: 2.08 ± 0.044	2.205GlnLys: 2.205 ± 0.048	2.257GlnLeu: 2.257 ± 0.043	0.772GlnMet: 0.772 ± 0.024	1.539GlnAsn: 1.539 ± 0.037	0.827GlnPro: 0.827 ± 0.026	0.91GlnGln: 0.91 ± 0.033	1.234GlnArg: 1.234 ± 0.036	1.691GlnSer: 1.691 ± 0.037	1.225GlnThr: 1.225 ± 0.036	1.625GlnVal: 1.625 ± 0.038	0.251GlnTrp: 0.251 ± 0.014	1.14GlnTyr: 1.14 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	2.327ArgAla: 2.327 ± 0.045	0.447ArgCys: 0.447 ± 0.018	2.21ArgAsp: 2.21 ± 0.044	3.075ArgGlu: 3.075 ± 0.055	1.7ArgPhe: 1.7 ± 0.038	2.321ArgGly: 2.321 ± 0.048	0.664ArgHis: 0.664 ± 0.023	3.556ArgIle: 3.556 ± 0.054	2.994ArgLys: 2.994 ± 0.049	3.879ArgLeu: 3.879 ± 0.061	1.285ArgMet: 1.285 ± 0.031	1.888ArgAsn: 1.888 ± 0.04	1.162ArgPro: 1.162 ± 0.033	1.309ArgGln: 1.309 ± 0.037	1.787ArgArg: 1.787 ± 0.04	1.987ArgSer: 1.987 ± 0.041	1.831ArgThr: 1.831 ± 0.039	2.487ArgVal: 2.487 ± 0.05	0.333ArgTrp: 0.333 ± 0.017	1.765ArgTyr: 1.765 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	4.081SerAla: 4.081 ± 0.063	0.752SerCys: 0.752 ± 0.024	3.197SerAsp: 3.197 ± 0.054	3.777SerGlu: 3.777 ± 0.055	3.129SerPhe: 3.129 ± 0.056	5.193SerGly: 5.193 ± 0.074	1.039SerHis: 1.039 ± 0.032	5.992SerIle: 5.992 ± 0.078	4.521SerLys: 4.521 ± 0.061	6.12SerLeu: 6.12 ± 0.081	1.906SerMet: 1.906 ± 0.037	2.951SerAsn: 2.951 ± 0.063	2.165SerPro: 2.165 ± 0.044	1.845SerGln: 1.845 ± 0.043	2.546SerArg: 2.546 ± 0.038	4.271SerSer: 4.271 ± 0.074	3.017SerThr: 3.017 ± 0.053	4.107SerVal: 4.107 ± 0.064	0.528SerTrp: 0.528 ± 0.021	2.481SerTyr: 2.481 ± 0.05	0.0SerXaa: 0.0 ± 0.0
Thr	4.21ThrAla: 4.21 ± 0.082	0.489ThrCys: 0.489 ± 0.022	2.784ThrAsp: 2.784 ± 0.048	3.031ThrGlu: 3.031 ± 0.049	2.06ThrPhe: 2.06 ± 0.037	4.616ThrGly: 4.616 ± 0.126	0.788ThrHis: 0.788 ± 0.023	4.406ThrIle: 4.406 ± 0.067	2.872ThrLys: 2.872 ± 0.046	4.708ThrLeu: 4.708 ± 0.064	1.281ThrMet: 1.281 ± 0.031	2.13ThrAsn: 2.13 ± 0.049	2.201ThrPro: 2.201 ± 0.055	1.316ThrGln: 1.316 ± 0.035	1.747ThrArg: 1.747 ± 0.038	3.06ThrSer: 3.06 ± 0.061	2.446ThrThr: 2.446 ± 0.051	4.002ThrVal: 4.002 ± 0.082	0.429ThrTrp: 0.429 ± 0.02	1.816ThrTyr: 1.816 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	4.187ValAla: 4.187 ± 0.069	0.864ValCys: 0.864 ± 0.027	3.647ValAsp: 3.647 ± 0.056	4.104ValGlu: 4.104 ± 0.062	2.993ValPhe: 2.993 ± 0.05	3.832ValGly: 3.832 ± 0.052	0.941ValHis: 0.941 ± 0.027	5.931ValIle: 5.931 ± 0.082	5.043ValLys: 5.043 ± 0.067	6.429ValLeu: 6.429 ± 0.079	1.85ValMet: 1.85 ± 0.037	3.355ValAsn: 3.355 ± 0.05	2.346ValPro: 2.346 ± 0.041	1.532ValGln: 1.532 ± 0.036	2.351ValArg: 2.351 ± 0.048	4.451ValSer: 4.451 ± 0.063	3.765ValThr: 3.765 ± 0.077	4.309ValVal: 4.309 ± 0.064	0.483ValTrp: 0.483 ± 0.024	2.551ValTyr: 2.551 ± 0.042	0.0ValXaa: 0.0 ± 0.0
Trp	0.515TrpAla: 0.515 ± 0.021	0.104TrpCys: 0.104 ± 0.009	0.5TrpAsp: 0.5 ± 0.021	0.567TrpGlu: 0.567 ± 0.023	0.375TrpPhe: 0.375 ± 0.017	0.619TrpGly: 0.619 ± 0.025	0.158TrpHis: 0.158 ± 0.009	0.657TrpIle: 0.657 ± 0.025	0.564TrpLys: 0.564 ± 0.02	0.754TrpLeu: 0.754 ± 0.026	0.274TrpMet: 0.274 ± 0.015	0.407TrpAsn: 0.407 ± 0.019	0.27TrpPro: 0.27 ± 0.014	0.275TrpGln: 0.275 ± 0.014	0.321TrpArg: 0.321 ± 0.016	0.476TrpSer: 0.476 ± 0.019	0.409TrpThr: 0.409 ± 0.018	0.543TrpVal: 0.543 ± 0.021	0.111TrpTrp: 0.111 ± 0.01	0.285TrpTyr: 0.285 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.35TyrAla: 2.35 ± 0.038	0.515TyrCys: 0.515 ± 0.02	2.495TyrAsp: 2.495 ± 0.053	2.729TyrGlu: 2.729 ± 0.045	2.023TyrPhe: 2.023 ± 0.043	2.932TyrGly: 2.932 ± 0.053	0.627TyrHis: 0.627 ± 0.022	3.764TyrIle: 3.764 ± 0.057	3.02TyrLys: 3.02 ± 0.053	3.585TyrLeu: 3.585 ± 0.055	1.094TyrMet: 1.094 ± 0.03	2.275TyrAsn: 2.275 ± 0.049	1.449TyrPro: 1.449 ± 0.036	0.992TyrGln: 0.992 ± 0.031	1.754TyrArg: 1.754 ± 0.04	2.703TyrSer: 2.703 ± 0.049	2.09TyrThr: 2.09 ± 0.047	2.251TyrVal: 2.251 ± 0.045	0.324TyrTrp: 0.324 ± 0.017	1.827TyrTyr: 1.827 ± 0.042	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4273 proteins (1282805 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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