Amino acid dipepetide frequency for Erythrobacter sp. HI0063

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.59AlaAla: 16.59 ± 0.197	1.123AlaCys: 1.123 ± 0.04	7.484AlaAsp: 7.484 ± 0.101	8.293AlaGlu: 8.293 ± 0.098	4.302AlaPhe: 4.302 ± 0.087	10.366AlaGly: 10.366 ± 0.123	2.174AlaHis: 2.174 ± 0.051	6.992AlaIle: 6.992 ± 0.09	4.324AlaLys: 4.324 ± 0.106	13.806AlaLeu: 13.806 ± 0.163	3.841AlaMet: 3.841 ± 0.056	3.199AlaAsn: 3.199 ± 0.063	5.71AlaPro: 5.71 ± 0.099	4.407AlaGln: 4.407 ± 0.069	9.598AlaArg: 9.598 ± 0.121	6.774AlaSer: 6.774 ± 0.112	5.866AlaThr: 5.866 ± 0.08	8.053AlaVal: 8.053 ± 0.105	1.628AlaTrp: 1.628 ± 0.044	2.456AlaTyr: 2.456 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	1.028CysAla: 1.028 ± 0.036	0.101CysCys: 0.101 ± 0.013	0.601CysAsp: 0.601 ± 0.025	0.527CysGlu: 0.527 ± 0.022	0.307CysPhe: 0.307 ± 0.019	0.828CysGly: 0.828 ± 0.033	0.193CysHis: 0.193 ± 0.016	0.331CysIle: 0.331 ± 0.021	0.192CysLys: 0.192 ± 0.015	0.714CysLeu: 0.714 ± 0.024	0.139CysMet: 0.139 ± 0.012	0.211CysAsn: 0.211 ± 0.016	0.434CysPro: 0.434 ± 0.023	0.237CysGln: 0.237 ± 0.014	0.503CysArg: 0.503 ± 0.027	0.486CysSer: 0.486 ± 0.022	0.394CysThr: 0.394 ± 0.021	0.565CysVal: 0.565 ± 0.028	0.075CysTrp: 0.075 ± 0.009	0.17CysTyr: 0.17 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	7.766AspAla: 7.766 ± 0.105	0.537AspCys: 0.537 ± 0.024	3.794AspAsp: 3.794 ± 0.08	4.364AspGlu: 4.364 ± 0.071	2.417AspPhe: 2.417 ± 0.058	5.944AspGly: 5.944 ± 0.088	1.347AspHis: 1.347 ± 0.043	3.054AspIle: 3.054 ± 0.057	1.826AspLys: 1.826 ± 0.049	6.359AspLeu: 6.359 ± 0.083	1.511AspMet: 1.511 ± 0.044	1.446AspAsn: 1.446 ± 0.042	3.983AspPro: 3.983 ± 0.069	1.876AspGln: 1.876 ± 0.049	5.46AspArg: 5.46 ± 0.074	2.551AspSer: 2.551 ± 0.061	3.043AspThr: 3.043 ± 0.059	3.906AspVal: 3.906 ± 0.069	1.184AspTrp: 1.184 ± 0.033	1.676AspTyr: 1.676 ± 0.043	0.0AspXaa: 0.0 ± 0.0
Glu	8.619GluAla: 8.619 ± 0.109	0.425GluCys: 0.425 ± 0.022	3.892GluAsp: 3.892 ± 0.071	4.248GluGlu: 4.248 ± 0.082	1.924GluPhe: 1.924 ± 0.053	5.387GluGly: 5.387 ± 0.081	1.221GluHis: 1.221 ± 0.039	3.354GluIle: 3.354 ± 0.074	2.412GluLys: 2.412 ± 0.058	5.673GluLeu: 5.673 ± 0.084	1.643GluMet: 1.643 ± 0.041	1.852GluAsn: 1.852 ± 0.048	3.067GluPro: 3.067 ± 0.064	2.354GluGln: 2.354 ± 0.054	5.924GluArg: 5.924 ± 0.097	2.649GluSer: 2.649 ± 0.049	3.787GluThr: 3.787 ± 0.069	3.836GluVal: 3.836 ± 0.07	0.96GluTrp: 0.96 ± 0.036	1.207GluTyr: 1.207 ± 0.036	0.001GluXaa: 0.001 ± 0.001
Phe	5.092PheAla: 5.092 ± 0.081	0.335PheCys: 0.335 ± 0.019	2.909PheAsp: 2.909 ± 0.057	2.413PheGlu: 2.413 ± 0.049	1.332PhePhe: 1.332 ± 0.044	3.694PheGly: 3.694 ± 0.076	0.634PheHis: 0.634 ± 0.027	1.391PheIle: 1.391 ± 0.043	0.841PheLys: 0.841 ± 0.033	3.242PheLeu: 3.242 ± 0.065	0.714PheMet: 0.714 ± 0.032	0.959PheAsn: 0.959 ± 0.039	1.476PhePro: 1.476 ± 0.036	0.914PheGln: 0.914 ± 0.035	2.124PheArg: 2.124 ± 0.041	2.066PheSer: 2.066 ± 0.05	1.991PheThr: 1.991 ± 0.043	2.806PheVal: 2.806 ± 0.057	0.532PheTrp: 0.532 ± 0.03	0.903PheTyr: 0.903 ± 0.032	0.001PheXaa: 0.001 ± 0.001
Gly	9.446GlyAla: 9.446 ± 0.12	0.814GlyCys: 0.814 ± 0.028	5.153GlyAsp: 5.153 ± 0.073	6.038GlyGlu: 6.038 ± 0.088	3.594GlyPhe: 3.594 ± 0.065	7.731GlyGly: 7.731 ± 0.125	1.775GlyHis: 1.775 ± 0.05	4.356GlyIle: 4.356 ± 0.076	3.089GlyLys: 3.089 ± 0.065	8.435GlyLeu: 8.435 ± 0.103	2.42GlyMet: 2.42 ± 0.064	2.208GlyAsn: 2.208 ± 0.053	3.627GlyPro: 3.627 ± 0.065	2.992GlyGln: 2.992 ± 0.064	6.151GlyArg: 6.151 ± 0.092	5.053GlySer: 5.053 ± 0.091	4.721GlyThr: 4.721 ± 0.085	5.853GlyVal: 5.853 ± 0.088	1.525GlyTrp: 1.525 ± 0.043	2.199GlyTyr: 2.199 ± 0.048	0.001GlyXaa: 0.001 ± 0.001
His	2.213HisAla: 2.213 ± 0.061	0.243HisCys: 0.243 ± 0.018	1.234HisAsp: 1.234 ± 0.041	1.139HisGlu: 1.139 ± 0.034	0.806HisPhe: 0.806 ± 0.03	1.828HisGly: 1.828 ± 0.044	0.529HisHis: 0.529 ± 0.028	0.873HisIle: 0.873 ± 0.034	0.481HisLys: 0.481 ± 0.023	1.782HisLeu: 1.782 ± 0.048	0.413HisMet: 0.413 ± 0.02	0.418HisAsn: 0.418 ± 0.024	1.148HisPro: 1.148 ± 0.037	0.496HisGln: 0.496 ± 0.025	1.45HisArg: 1.45 ± 0.039	0.978HisSer: 0.978 ± 0.033	0.744HisThr: 0.744 ± 0.03	1.277HisVal: 1.277 ± 0.038	0.329HisTrp: 0.329 ± 0.019	0.492HisTyr: 0.492 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	8.079IleAla: 8.079 ± 0.108	0.442IleCys: 0.442 ± 0.024	3.912IleAsp: 3.912 ± 0.065	3.964IleGlu: 3.964 ± 0.067	1.604IlePhe: 1.604 ± 0.045	5.144IleGly: 5.144 ± 0.08	0.802IleHis: 0.802 ± 0.032	1.715IleIle: 1.715 ± 0.046	1.169IleLys: 1.169 ± 0.039	4.114IleLeu: 4.114 ± 0.08	0.907IleMet: 0.907 ± 0.032	1.241IleAsn: 1.241 ± 0.037	2.267IlePro: 2.267 ± 0.054	1.065IleGln: 1.065 ± 0.039	3.103IleArg: 3.103 ± 0.059	2.428IleSer: 2.428 ± 0.051	2.317IleThr: 2.317 ± 0.053	4.081IleVal: 4.081 ± 0.072	0.605IleTrp: 0.605 ± 0.026	0.986IleTyr: 0.986 ± 0.036	0.0IleXaa: 0.0 ± 0.0
Lys	3.823LysAla: 3.823 ± 0.084	0.176LysCys: 0.176 ± 0.013	1.788LysAsp: 1.788 ± 0.053	1.505LysGlu: 1.505 ± 0.045	0.87LysPhe: 0.87 ± 0.028	2.65LysGly: 2.65 ± 0.06	0.596LysHis: 0.596 ± 0.026	1.433LysIle: 1.433 ± 0.042	1.237LysLys: 1.237 ± 0.055	3.164LysLeu: 3.164 ± 0.062	0.74LysMet: 0.74 ± 0.03	0.758LysAsn: 0.758 ± 0.032	1.916LysPro: 1.916 ± 0.047	0.958LysGln: 0.958 ± 0.037	2.537LysArg: 2.537 ± 0.057	1.658LysSer: 1.658 ± 0.047	1.762LysThr: 1.762 ± 0.048	2.057LysVal: 2.057 ± 0.054	0.397LysTrp: 0.397 ± 0.02	0.583LysTyr: 0.583 ± 0.026	0.0LysXaa: 0.0 ± 0.0
Leu	14.297LeuAla: 14.297 ± 0.177	0.737LeuCys: 0.737 ± 0.029	6.629LeuAsp: 6.629 ± 0.097	5.69LeuGlu: 5.69 ± 0.085	3.615LeuPhe: 3.615 ± 0.077	8.117LeuGly: 8.117 ± 0.103	1.707LeuHis: 1.707 ± 0.044	4.608LeuIle: 4.608 ± 0.079	2.945LeuLys: 2.945 ± 0.065	8.979LeuLeu: 8.979 ± 0.141	2.176LeuMet: 2.176 ± 0.054	2.244LeuAsn: 2.244 ± 0.048	5.285LeuPro: 5.285 ± 0.076	2.426LeuGln: 2.426 ± 0.055	6.797LeuArg: 6.797 ± 0.107	5.954LeuSer: 5.954 ± 0.083	5.438LeuThr: 5.438 ± 0.088	7.35LeuVal: 7.35 ± 0.099	1.184LeuTrp: 1.184 ± 0.037	1.942LeuTyr: 1.942 ± 0.052	0.001LeuXaa: 0.001 ± 0.001
Met	3.262MetAla: 3.262 ± 0.062	0.135MetCys: 0.135 ± 0.012	1.326MetAsp: 1.326 ± 0.042	1.273MetGlu: 1.273 ± 0.039	0.624MetPhe: 0.624 ± 0.028	1.96MetGly: 1.96 ± 0.051	0.459MetHis: 0.459 ± 0.024	1.334MetIle: 1.334 ± 0.042	0.946MetLys: 0.946 ± 0.033	2.557MetLeu: 2.557 ± 0.064	0.691MetMet: 0.691 ± 0.032	0.673MetAsn: 0.673 ± 0.026	1.497MetPro: 1.497 ± 0.038	0.791MetGln: 0.791 ± 0.029	1.938MetArg: 1.938 ± 0.049	1.41MetSer: 1.41 ± 0.039	1.604MetThr: 1.604 ± 0.05	1.622MetVal: 1.622 ± 0.044	0.223MetTrp: 0.223 ± 0.015	0.264MetTyr: 0.264 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.275AsnAla: 3.275 ± 0.062	0.238AsnCys: 0.238 ± 0.016	1.422AsnAsp: 1.422 ± 0.037	1.339AsnGlu: 1.339 ± 0.038	0.962AsnPhe: 0.962 ± 0.038	2.338AsnGly: 2.338 ± 0.056	0.482AsnHis: 0.482 ± 0.022	1.222AsnIle: 1.222 ± 0.044	0.598AsnLys: 0.598 ± 0.029	2.471AsnLeu: 2.471 ± 0.062	0.546AsnMet: 0.546 ± 0.025	0.6AsnAsn: 0.6 ± 0.033	1.794AsnPro: 1.794 ± 0.048	0.722AsnGln: 0.722 ± 0.029	1.979AsnArg: 1.979 ± 0.045	1.304AsnSer: 1.304 ± 0.04	1.166AsnThr: 1.166 ± 0.042	1.739AsnVal: 1.739 ± 0.05	0.383AsnTrp: 0.383 ± 0.021	0.619AsnTyr: 0.619 ± 0.027	0.0AsnXaa: 0.0 ± 0.0
Pro	5.833ProAla: 5.833 ± 0.095	0.331ProCys: 0.331 ± 0.022	4.035ProAsp: 4.035 ± 0.072	3.991ProGlu: 3.991 ± 0.077	1.92ProPhe: 1.92 ± 0.045	4.432ProGly: 4.432 ± 0.073	0.989ProHis: 0.989 ± 0.032	2.55ProIle: 2.55 ± 0.056	1.49ProLys: 1.49 ± 0.04	4.675ProLeu: 4.675 ± 0.087	1.163ProMet: 1.163 ± 0.037	1.319ProAsn: 1.319 ± 0.041	2.537ProPro: 2.537 ± 0.085	1.746ProGln: 1.746 ± 0.047	2.959ProArg: 2.959 ± 0.062	2.805ProSer: 2.805 ± 0.061	2.425ProThr: 2.425 ± 0.06	4.039ProVal: 4.039 ± 0.072	0.635ProTrp: 0.635 ± 0.03	1.097ProTyr: 1.097 ± 0.04	0.0ProXaa: 0.0 ± 0.0
Gln	4.172GlnAla: 4.172 ± 0.07	0.2GlnCys: 0.2 ± 0.016	1.737GlnAsp: 1.737 ± 0.052	1.5GlnGlu: 1.5 ± 0.037	1.177GlnPhe: 1.177 ± 0.035	2.377GlnGly: 2.377 ± 0.056	0.558GlnHis: 0.558 ± 0.026	1.734GlnIle: 1.734 ± 0.048	0.88GlnLys: 0.88 ± 0.034	2.972GlnLeu: 2.972 ± 0.058	0.788GlnMet: 0.788 ± 0.026	0.791GlnAsn: 0.791 ± 0.032	1.672GlnPro: 1.672 ± 0.041	1.093GlnGln: 1.093 ± 0.051	2.541GlnArg: 2.541 ± 0.056	1.752GlnSer: 1.752 ± 0.037	1.7GlnThr: 1.7 ± 0.041	2.218GlnVal: 2.218 ± 0.044	0.412GlnTrp: 0.412 ± 0.023	0.623GlnTyr: 0.623 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	8.544ArgAla: 8.544 ± 0.114	0.48ArgCys: 0.48 ± 0.024	4.62ArgAsp: 4.62 ± 0.076	4.932ArgGlu: 4.932 ± 0.085	3.109ArgPhe: 3.109 ± 0.058	5.186ArgGly: 5.186 ± 0.076	1.575ArgHis: 1.575 ± 0.048	4.385ArgIle: 4.385 ± 0.068	2.278ArgLys: 2.278 ± 0.056	7.825ArgLeu: 7.825 ± 0.118	2.061ArgMet: 2.061 ± 0.051	1.858ArgAsn: 1.858 ± 0.041	3.41ArgPro: 3.41 ± 0.068	2.494ArgGln: 2.494 ± 0.056	5.596ArgArg: 5.596 ± 0.095	4.045ArgSer: 4.045 ± 0.074	3.632ArgThr: 3.632 ± 0.064	4.702ArgVal: 4.702 ± 0.079	1.168ArgTrp: 1.168 ± 0.037	1.801ArgTyr: 1.801 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	6.317SerAla: 6.317 ± 0.099	0.402SerCys: 0.402 ± 0.02	3.728SerAsp: 3.728 ± 0.065	3.467SerGlu: 3.467 ± 0.058	2.152SerPhe: 2.152 ± 0.047	5.535SerGly: 5.535 ± 0.085	0.927SerHis: 0.927 ± 0.029	2.564SerIle: 2.564 ± 0.056	1.532SerLys: 1.532 ± 0.046	5.116SerLeu: 5.116 ± 0.088	1.204SerMet: 1.204 ± 0.037	1.391SerAsn: 1.391 ± 0.044	2.689SerPro: 2.689 ± 0.058	1.695SerGln: 1.695 ± 0.035	3.595SerArg: 3.595 ± 0.07	2.925SerSer: 2.925 ± 0.068	2.493SerThr: 2.493 ± 0.062	3.692SerVal: 3.692 ± 0.065	0.78SerTrp: 0.78 ± 0.031	1.305SerTyr: 1.305 ± 0.037	0.0SerXaa: 0.0 ± 0.0
Thr	6.143ThrAla: 6.143 ± 0.093	0.4ThrCys: 0.4 ± 0.026	3.051ThrAsp: 3.051 ± 0.058	2.726ThrGlu: 2.726 ± 0.06	1.841ThrPhe: 1.841 ± 0.05	5.159ThrGly: 5.159 ± 0.082	0.905ThrHis: 0.905 ± 0.034	2.908ThrIle: 2.908 ± 0.059	1.395ThrLys: 1.395 ± 0.041	5.562ThrLeu: 5.562 ± 0.076	1.238ThrMet: 1.238 ± 0.04	1.291ThrAsn: 1.291 ± 0.041	3.034ThrPro: 3.034 ± 0.056	1.499ThrGln: 1.499 ± 0.042	3.492ThrArg: 3.492 ± 0.063	2.618ThrSer: 2.618 ± 0.061	2.442ThrThr: 2.442 ± 0.058	4.086ThrVal: 4.086 ± 0.069	0.558ThrTrp: 0.558 ± 0.026	1.208ThrTyr: 1.208 ± 0.033	0.0ThrXaa: 0.0 ± 0.0
Val	8.665ValAla: 8.665 ± 0.121	0.568ValCys: 0.568 ± 0.026	4.306ValAsp: 4.306 ± 0.071	4.974ValGlu: 4.974 ± 0.08	2.357ValPhe: 2.357 ± 0.055	5.43ValGly: 5.43 ± 0.081	1.208ValHis: 1.208 ± 0.039	3.584ValIle: 3.584 ± 0.065	2.018ValLys: 2.018 ± 0.05	6.754ValLeu: 6.754 ± 0.097	1.691ValMet: 1.691 ± 0.046	1.726ValAsn: 1.726 ± 0.054	3.727ValPro: 3.727 ± 0.062	1.924ValGln: 1.924 ± 0.046	4.786ValArg: 4.786 ± 0.066	3.937ValSer: 3.937 ± 0.071	4.248ValThr: 4.248 ± 0.083	5.231ValVal: 5.231 ± 0.095	0.862ValTrp: 0.862 ± 0.032	1.274ValTyr: 1.274 ± 0.036	0.0ValXaa: 0.0 ± 0.0
Trp	1.316TrpAla: 1.316 ± 0.041	0.123TrpCys: 0.123 ± 0.011	0.792TrpAsp: 0.792 ± 0.03	0.646TrpGlu: 0.646 ± 0.029	0.632TrpPhe: 0.632 ± 0.026	0.927TrpGly: 0.927 ± 0.033	0.354TrpHis: 0.354 ± 0.021	0.747TrpIle: 0.747 ± 0.032	0.465TrpLys: 0.465 ± 0.025	1.751TrpLeu: 1.751 ± 0.053	0.355TrpMet: 0.355 ± 0.022	0.444TrpAsn: 0.444 ± 0.026	0.651TrpPro: 0.651 ± 0.028	0.595TrpGln: 0.595 ± 0.03	1.284TrpArg: 1.284 ± 0.033	0.856TrpSer: 0.856 ± 0.031	0.783TrpThr: 0.783 ± 0.028	0.766TrpVal: 0.766 ± 0.033	0.246TrpTrp: 0.246 ± 0.018	0.273TrpTyr: 0.273 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.5TyrAla: 2.5 ± 0.051	0.225TyrCys: 0.225 ± 0.016	1.631TyrAsp: 1.631 ± 0.049	1.407TyrGlu: 1.407 ± 0.037	0.826TyrPhe: 0.826 ± 0.032	2.15TyrGly: 2.15 ± 0.058	0.436TyrHis: 0.436 ± 0.021	0.812TyrIle: 0.812 ± 0.031	0.49TyrLys: 0.49 ± 0.025	2.169TyrLeu: 2.169 ± 0.048	0.347TyrMet: 0.347 ± 0.019	0.572TyrAsn: 0.572 ± 0.028	1.047TyrPro: 1.047 ± 0.036	0.612TyrGln: 0.612 ± 0.027	1.884TyrArg: 1.884 ± 0.045	1.26TyrSer: 1.26 ± 0.04	0.997TyrThr: 0.997 ± 0.036	1.392TyrVal: 1.392 ± 0.043	0.322TyrTrp: 0.322 ± 0.021	0.614TyrTyr: 0.614 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.003XaaAla: 0.003 ± 0.003	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.001	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 2915 proteins (915420 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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