Po-Circo-like virus 21

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae; Po-Circo-like virus

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8E3X6|G8E3X6_9CIRC Uncharacterized protein OS=Po-Circo-like virus 21 OX=1105383 PE=4 SV=1
MM1 pKa = 7.17SQDD4 pKa = 3.26FDD6 pKa = 4.02VFDD9 pKa = 5.08PFSDD13 pKa = 3.92YY14 pKa = 11.42CPDD17 pKa = 3.88FSQYY21 pKa = 11.34GFDD24 pKa = 3.64EE25 pKa = 4.94ASSQVLDD32 pKa = 4.01PSGNPYY38 pKa = 7.81EE39 pKa = 4.36TQITVPFLQSVEE51 pKa = 4.2QGSDD55 pKa = 3.09PSVTLRR61 pKa = 11.84SMLEE65 pKa = 3.74QEE67 pKa = 4.17YY68 pKa = 10.16HH69 pKa = 5.73SNRR72 pKa = 11.84RR73 pKa = 11.84EE74 pKa = 3.95VTDD77 pKa = 3.72PTDD80 pKa = 4.38VMPQDD85 pKa = 3.52APVFRR90 pKa = 11.84RR91 pKa = 11.84LDD93 pKa = 3.59EE94 pKa = 4.38NSVLFHH100 pKa = 7.12DD101 pKa = 4.71VSFAGPTLRR110 pKa = 11.84SEE112 pKa = 4.47QQCKK116 pKa = 7.53TRR118 pKa = 11.84EE119 pKa = 4.05LQGFLILPARR129 pKa = 11.84FCTAIYY135 pKa = 10.45SRR137 pKa = 11.84AGNHH141 pKa = 6.03SLPTPALFVKK151 pKa = 10.16EE152 pKa = 4.22LDD154 pKa = 3.78SSISALFEE162 pKa = 3.97

Molecular weight: 18.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8E3X4|G8E3X4_9CIRC Uncharacterized protein OS=Po-Circo-like virus 21 OX=1105383 PE=4 SV=1
MM1 pKa = 7.32SAIISLASSSLSLHH15 pKa = 6.19FSSFVSLSFSFSTITWSSFVQYY37 pKa = 10.82FMLSISDD44 pKa = 5.0PIATSRR50 pKa = 11.84CALPPSHH57 pKa = 6.98LCITVMSFFVFSLKK71 pKa = 10.49YY72 pKa = 9.94PCICGVPSSRR82 pKa = 11.84CSSSAITASTSGFNSRR98 pKa = 11.84FLTASSSSS106 pKa = 3.3

Molecular weight: 11.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

859

106

310

171.8

19.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.752 ± 0.528

1.863 ± 0.469

5.006 ± 1.062

5.937 ± 2.172

5.588 ± 1.088

4.773 ± 0.595

1.746 ± 0.528

5.588 ± 0.852

4.657 ± 1.362

7.451 ± 0.555

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.328 ± 0.409

5.122 ± 1.055

5.239 ± 0.785

4.889 ± 0.688

4.773 ± 0.751

9.895 ± 3.891

6.519 ± 0.592

6.286 ± 1.04

1.513 ± 0.751

4.075 ± 0.493
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski