Streptococcus phage IPP15

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5S926|A0A1S5S926_9CAUD Major tail protein OS=Streptococcus phage IPP15 OX=1916155 GN=IPP15_00045 PE=4 SV=1
MM1 pKa = 7.91AILDD5 pKa = 4.57DD6 pKa = 4.75LQALYY11 pKa = 11.25DD12 pKa = 4.06NGWDD16 pKa = 4.07ASFNYY21 pKa = 9.53NGQVCGIFPNSIYY34 pKa = 10.59DD35 pKa = 3.4IVVIIADD42 pKa = 3.56NEE44 pKa = 3.99YY45 pKa = 10.1RR46 pKa = 11.84ASSFDD51 pKa = 4.4DD52 pKa = 4.95LISLQIEE59 pKa = 4.74GKK61 pKa = 7.71TLPEE65 pKa = 3.62IMNEE69 pKa = 3.98VEE71 pKa = 4.11VQYY74 pKa = 11.6GG75 pKa = 3.21

Molecular weight: 8.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5S8P7|A0A1S5S8P7_9CAUD Uncharacterized protein OS=Streptococcus phage IPP15 OX=1916155 GN=IPP15_00022 PE=4 SV=1
MM1 pKa = 7.37KK2 pKa = 10.24RR3 pKa = 11.84FIAIWILLSAGLNIWQSIYY22 pKa = 10.34IKK24 pKa = 10.39KK25 pKa = 10.43LEE27 pKa = 4.24EE28 pKa = 3.63KK29 pKa = 10.59RR30 pKa = 11.84PIVVYY35 pKa = 10.42KK36 pKa = 10.53ADD38 pKa = 3.62NAGVEE43 pKa = 4.16IFGKK47 pKa = 9.68VVEE50 pKa = 4.66KK51 pKa = 10.79GRR53 pKa = 11.84HH54 pKa = 4.57GKK56 pKa = 10.52LYY58 pKa = 9.59TLTIRR63 pKa = 11.84DD64 pKa = 3.55YY65 pKa = 11.71GVFVVTKK72 pKa = 10.36DD73 pKa = 3.26VYY75 pKa = 11.6DD76 pKa = 3.62KK77 pKa = 11.58VKK79 pKa = 11.11VGDD82 pKa = 3.84EE83 pKa = 4.1VLLL86 pKa = 4.2

Molecular weight: 9.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

12023

52

2127

218.6

24.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.97 ± 0.622

0.599 ± 0.128

6.313 ± 0.299

7.852 ± 0.506

4.15 ± 0.216

6.546 ± 0.498

1.372 ± 0.156

6.571 ± 0.305

8.234 ± 0.362

7.91 ± 0.293

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.196 ± 0.215

5.914 ± 0.308

2.728 ± 0.264

4.317 ± 0.309

4.541 ± 0.327

5.972 ± 0.388

6.038 ± 0.369

6.454 ± 0.289

1.389 ± 0.13

3.934 ± 0.366
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski