Streptococcus satellite phage Javan561

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4D5ZVU0|A0A4D5ZVU0_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan561 OX=2558750 GN=JavanS561_0008 PE=4 SV=1
MM1 pKa = 6.48TTLEE5 pKa = 3.79EE6 pKa = 4.57RR7 pKa = 11.84YY8 pKa = 10.01CIEE11 pKa = 3.34RR12 pKa = 11.84RR13 pKa = 11.84AFLGQRR19 pKa = 11.84ATRR22 pKa = 11.84LEE24 pKa = 3.83LLEE27 pKa = 3.89RR28 pKa = 11.84VIRR31 pKa = 11.84LEE33 pKa = 4.49LMLMDD38 pKa = 4.78IADD41 pKa = 3.98STLDD45 pKa = 3.22TDD47 pKa = 4.36FPEE50 pKa = 4.42MAVNTTLDD58 pKa = 3.82HH59 pKa = 6.56IADD62 pKa = 4.41FGDD65 pKa = 3.32EE66 pKa = 4.04ALYY69 pKa = 10.92LLEE72 pKa = 5.43SLDD75 pKa = 3.97HH76 pKa = 6.98DD77 pKa = 4.84GDD79 pKa = 4.35YY80 pKa = 10.88KK81 pKa = 11.34ALWEE85 pKa = 4.2HH86 pKa = 6.03TKK88 pKa = 9.84DD89 pKa = 3.16TGADD93 pKa = 3.13NGKK96 pKa = 9.96

Molecular weight:
11.06 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4D5ZQW0|A0A4D5ZQW0_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan561 OX=2558750 GN=JavanS561_0011 PE=4 SV=1
MM1 pKa = 7.19NAVEE5 pKa = 5.08PIRR8 pKa = 11.84DD9 pKa = 3.55KK10 pKa = 11.73DD11 pKa = 4.28DD12 pKa = 3.22IQAMKK17 pKa = 10.58DD18 pKa = 3.51YY19 pKa = 10.63LRR21 pKa = 11.84SWNEE25 pKa = 3.0RR26 pKa = 11.84NYY28 pKa = 10.4ILFLVGINTGFRR40 pKa = 11.84IGDD43 pKa = 3.57ILKK46 pKa = 10.51LRR48 pKa = 11.84VKK50 pKa = 10.27DD51 pKa = 3.56VQGWHH56 pKa = 5.22IRR58 pKa = 11.84VRR60 pKa = 11.84EE61 pKa = 3.95QKK63 pKa = 8.96TGKK66 pKa = 10.01QKK68 pKa = 9.27TIKK71 pKa = 7.85MTRR74 pKa = 11.84TLKK77 pKa = 10.84NEE79 pKa = 3.24LRR81 pKa = 11.84KK82 pKa = 9.86YY83 pKa = 10.75VEE85 pKa = 4.46GKK87 pKa = 9.28PLHH90 pKa = 6.77HH91 pKa = 6.92YY92 pKa = 10.12LFQSRR97 pKa = 11.84NGVNKK102 pKa = 9.67PLDD105 pKa = 3.47RR106 pKa = 11.84RR107 pKa = 11.84TVYY110 pKa = 10.13WILKK114 pKa = 7.53TAGNDD119 pKa = 3.58LGIEE123 pKa = 4.31NIGTHH128 pKa = 5.09TMRR131 pKa = 11.84KK132 pKa = 6.7TFGYY136 pKa = 9.98HH137 pKa = 5.21YY138 pKa = 9.53YY139 pKa = 10.76KK140 pKa = 10.53KK141 pKa = 10.06YY142 pKa = 11.08KK143 pKa = 10.39NVADD147 pKa = 3.87LMSIFNHH154 pKa = 6.08SSPAITLIYY163 pKa = 10.12IGVRR167 pKa = 11.84QDD169 pKa = 4.72DD170 pKa = 5.37LDD172 pKa = 3.68TKK174 pKa = 10.06MANFDD179 pKa = 3.22II180 pKa = 5.15

Molecular weight:
21.25 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16

0

16

2508

42

510

156.8

18.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.702 ± 0.394

0.678 ± 0.189

5.941 ± 0.43

8.373 ± 1.166

4.625 ± 0.557

4.944 ± 0.57

2.273 ± 0.282

6.818 ± 0.586

9.49 ± 0.735

10.287 ± 0.828

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.313 ± 0.466

5.343 ± 0.672

2.751 ± 0.617

4.545 ± 0.464

4.984 ± 0.511

4.226 ± 0.349

6.18 ± 0.551

4.785 ± 0.259

1.116 ± 0.185

4.625 ± 0.263

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski