Sphingomonas sp. NFR15

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4025 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G5UC28|A0A1G5UC28_9SPHN Acetolactate synthase-1/2/3 large subunit OS=Sphingomonas sp. NFR15 OX=1566282 GN=SAMN03159340_02582 PE=3 SV=1
MM1 pKa = 7.28SVAGKK6 pKa = 8.91WRR8 pKa = 11.84IVAMPGYY15 pKa = 9.5PDD17 pKa = 5.39DD18 pKa = 4.96YY19 pKa = 10.9PDD21 pKa = 3.97LVEE24 pKa = 3.77PAYY27 pKa = 9.91ILFEE31 pKa = 4.1NAGGGEE37 pKa = 4.26FAFGACTGNIWEE49 pKa = 4.76ASSANATSIDD59 pKa = 3.93FSWDD63 pKa = 2.85GSDD66 pKa = 5.71EE67 pKa = 3.95MDD69 pKa = 4.01EE70 pKa = 4.32VCGDD74 pKa = 3.86GDD76 pKa = 5.73AEE78 pKa = 4.12LQPDD82 pKa = 4.05GSLRR86 pKa = 11.84GDD88 pKa = 2.69IRR90 pKa = 11.84YY91 pKa = 9.96RR92 pKa = 11.84NGDD95 pKa = 4.38EE96 pKa = 4.12IPFIATKK103 pKa = 9.39WLSSTACC110 pKa = 3.65

Molecular weight: 11.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G5TIS2|A0A1G5TIS2_9SPHN Outer membrane immunogenic protein OS=Sphingomonas sp. NFR15 OX=1566282 GN=SAMN03159340_01407 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.35GFRR19 pKa = 11.84ARR21 pKa = 11.84MEE23 pKa = 4.4TPGGRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4025

0

4025

1327836

39

1932

329.9

35.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.229 ± 0.068

0.709 ± 0.011

6.025 ± 0.03

4.855 ± 0.039

3.524 ± 0.024

8.934 ± 0.041

2.024 ± 0.019

4.913 ± 0.023

2.682 ± 0.027

9.878 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.179 ± 0.019

2.387 ± 0.03

5.51 ± 0.03

2.871 ± 0.022

7.589 ± 0.039

4.998 ± 0.029

5.609 ± 0.031

7.377 ± 0.03

1.448 ± 0.016

2.259 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski