Mycobacterium phage LastHope

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 102 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A222ZR90|A0A222ZR90_9CAUD Uncharacterized protein OS=Mycobacterium phage LastHope OX=2015886 GN=39 PE=4 SV=1
MM1 pKa = 7.6SIAEE5 pKa = 4.14QYY7 pKa = 8.31PARR10 pKa = 11.84TDD12 pKa = 3.26TNGRR16 pKa = 11.84TWYY19 pKa = 10.31RR20 pKa = 11.84PVKK23 pKa = 10.42AAGLDD28 pKa = 3.51MSQWGWTSDD37 pKa = 3.39PTQAHH42 pKa = 7.23PDD44 pKa = 3.31YY45 pKa = 11.01DD46 pKa = 3.81ALNTCTCPYY55 pKa = 10.07VDD57 pKa = 3.6PSLWTTHH64 pKa = 5.6YY65 pKa = 10.81GAVEE69 pKa = 4.24PGSQMEE75 pKa = 4.74HH76 pKa = 6.35NPLCPVHH83 pKa = 6.89PATLVDD89 pKa = 3.88LVVARR94 pKa = 11.84EE95 pKa = 4.23APVVAAAAEE104 pKa = 4.04RR105 pKa = 11.84AEE107 pKa = 4.41VYY109 pKa = 10.73GDD111 pKa = 2.97AWAQVFEE118 pKa = 4.47LAGGGCAIVPVDD130 pKa = 4.32PLPVEE135 pKa = 4.3PVGEE139 pKa = 4.9LGDD142 pKa = 3.45EE143 pKa = 4.37WVQLGYY149 pKa = 10.75VDD151 pKa = 3.99EE152 pKa = 4.66TQGGLWW158 pKa = 3.22

Molecular weight: 17.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A222ZTE0|A0A222ZTE0_9CAUD Uncharacterized protein OS=Mycobacterium phage LastHope OX=2015886 GN=93 PE=4 SV=1
MM1 pKa = 7.49TMTTATRR8 pKa = 11.84RR9 pKa = 11.84MSQMEE14 pKa = 3.89AHH16 pKa = 6.59QITVGLIRR24 pKa = 11.84EE25 pKa = 4.36HH26 pKa = 7.54GLIGWTVAWDD36 pKa = 3.54NARR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84AGQCRR46 pKa = 11.84YY47 pKa = 6.6STRR50 pKa = 11.84TISLSRR56 pKa = 11.84ALLRR60 pKa = 11.84QRR62 pKa = 11.84SYY64 pKa = 11.59DD65 pKa = 3.58DD66 pKa = 3.0TMMTITHH73 pKa = 7.35EE74 pKa = 4.05IAHH77 pKa = 6.47ALVGPKK83 pKa = 9.69HH84 pKa = 5.39GHH86 pKa = 5.05NAVWAAKK93 pKa = 9.61HH94 pKa = 6.16RR95 pKa = 11.84EE96 pKa = 4.02LGGNGQRR103 pKa = 11.84CFEE106 pKa = 4.86HH107 pKa = 8.53LDD109 pKa = 3.64EE110 pKa = 4.72SAPWMGTCSHH120 pKa = 6.25GKK122 pKa = 9.54KK123 pKa = 8.95FARR126 pKa = 11.84YY127 pKa = 7.72RR128 pKa = 11.84APKK131 pKa = 10.21RR132 pKa = 11.84LDD134 pKa = 2.86GWRR137 pKa = 11.84CRR139 pKa = 11.84CAAGGTPIVWANQRR153 pKa = 3.38

Molecular weight: 17.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

102

0

102

19087

37

1375

187.1

20.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.899 ± 0.455

1.126 ± 0.141

6.591 ± 0.268

5.732 ± 0.273

2.572 ± 0.158

9.027 ± 0.391

2.253 ± 0.157

3.887 ± 0.174

3.065 ± 0.182

8.205 ± 0.245

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.263 ± 0.097

2.688 ± 0.183

5.533 ± 0.241

3.285 ± 0.128

7.319 ± 0.369

4.841 ± 0.196

6.015 ± 0.196

8.215 ± 0.271

2.09 ± 0.088

2.394 ± 0.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski