Pusillimonas sp. JR1/69-2-13

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Alcaligenaceae; Pusillimonas; unclassified Pusillimonas

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3981 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N4UFQ7|A0A2N4UFQ7_9BURK HAD family hydrolase OS=Pusillimonas sp. JR1/69-2-13 OX=2045209 GN=CR155_10370 PE=4 SV=1
MM1 pKa = 6.51THH3 pKa = 6.28VVTEE7 pKa = 3.99NCIKK11 pKa = 10.76CKK13 pKa = 9.68FTDD16 pKa = 4.08CVDD19 pKa = 3.58VCPVDD24 pKa = 4.07CFRR27 pKa = 11.84EE28 pKa = 4.39GANFLVIDD36 pKa = 4.42PDD38 pKa = 3.65EE39 pKa = 5.79CIDD42 pKa = 4.05CAVCVPEE49 pKa = 5.1CPANAIFAEE58 pKa = 4.18EE59 pKa = 5.26DD60 pKa = 3.65VPQDD64 pKa = 3.29QVQFIALNAEE74 pKa = 4.55LSPEE78 pKa = 3.55FGMINRR84 pKa = 11.84SRR86 pKa = 11.84QPLPGADD93 pKa = 3.42DD94 pKa = 3.75WNGVDD99 pKa = 6.08DD100 pKa = 4.03KK101 pKa = 11.63LKK103 pKa = 10.12YY104 pKa = 10.48LEE106 pKa = 4.2RR107 pKa = 5.83

Molecular weight: 11.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N4UL46|A0A2N4UL46_9BURK Chromosome partitioning protein ParB OS=Pusillimonas sp. JR1/69-2-13 OX=2045209 GN=CR155_01420 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.39TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3981

0

3981

1287452

37

2838

323.4

35.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.259 ± 0.037

0.943 ± 0.012

5.352 ± 0.025

5.219 ± 0.032

3.582 ± 0.024

8.046 ± 0.036

2.289 ± 0.018

5.456 ± 0.028

3.561 ± 0.032

10.556 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.675 ± 0.019

2.997 ± 0.019

4.991 ± 0.024

3.962 ± 0.024

6.385 ± 0.036

5.993 ± 0.024

5.335 ± 0.023

7.534 ± 0.037

1.374 ± 0.016

2.491 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski