Amino acid dipepetide frequency for Gordonia phage Gibbles

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.62AlaAla: 10.62 ± 2.993
0.408AlaCys: 0.408 ± 0.138
4.656AlaAsp: 4.656 ± 0.442
3.962AlaGlu: 3.962 ± 0.367
2.9AlaPhe: 2.9 ± 0.283
6.29AlaGly: 6.29 ± 1.149
1.307AlaHis: 1.307 ± 0.214
4.942AlaIle: 4.942 ± 0.375
4.697AlaLys: 4.697 ± 0.735
7.148AlaLeu: 7.148 ± 0.746
2.246AlaMet: 2.246 ± 0.369
3.635AlaAsn: 3.635 ± 0.359
5.146AlaPro: 5.146 ± 0.841
3.594AlaGln: 3.594 ± 0.604
4.166AlaArg: 4.166 ± 0.332
4.983AlaSer: 4.983 ± 0.539
5.882AlaThr: 5.882 ± 0.766
6.004AlaVal: 6.004 ± 0.622
1.348AlaTrp: 1.348 ± 0.268
2.9AlaTyr: 2.9 ± 0.373
0.0AlaXaa: 0.0 ± 0.0
Cys
0.654CysAla: 0.654 ± 0.186
0.041CysCys: 0.041 ± 0.043
0.613CysAsp: 0.613 ± 0.17
0.776CysGlu: 0.776 ± 0.206
0.449CysPhe: 0.449 ± 0.142
0.735CysGly: 0.735 ± 0.198
0.245CysHis: 0.245 ± 0.106
0.572CysIle: 0.572 ± 0.148
0.613CysLys: 0.613 ± 0.186
0.776CysLeu: 0.776 ± 0.204
0.245CysMet: 0.245 ± 0.098
0.531CysAsn: 0.531 ± 0.166
0.654CysPro: 0.654 ± 0.211
0.204CysGln: 0.204 ± 0.089
0.368CysArg: 0.368 ± 0.104
0.776CysSer: 0.776 ± 0.187
0.449CysThr: 0.449 ± 0.128
0.613CysVal: 0.613 ± 0.211
0.082CysTrp: 0.082 ± 0.062
0.327CysTyr: 0.327 ± 0.119
0.0CysXaa: 0.0 ± 0.0
Asp
5.065AspAla: 5.065 ± 0.779
0.654AspCys: 0.654 ± 0.145
4.248AspAsp: 4.248 ± 0.658
5.106AspGlu: 5.106 ± 0.434
2.614AspPhe: 2.614 ± 0.35
4.615AspGly: 4.615 ± 0.587
1.225AspHis: 1.225 ± 0.244
4.33AspIle: 4.33 ± 0.414
3.88AspLys: 3.88 ± 0.341
3.921AspLeu: 3.921 ± 0.526
1.675AspMet: 1.675 ± 0.249
3.431AspAsn: 3.431 ± 0.512
3.635AspPro: 3.635 ± 0.447
2.001AspGln: 2.001 ± 0.289
2.655AspArg: 2.655 ± 0.391
4.084AspSer: 4.084 ± 0.424
3.921AspThr: 3.921 ± 0.39
3.799AspVal: 3.799 ± 0.342
1.348AspTrp: 1.348 ± 0.207
2.369AspTyr: 2.369 ± 0.344
0.0AspXaa: 0.0 ± 0.0
Glu
3.962GluAla: 3.962 ± 0.434
0.817GluCys: 0.817 ± 0.216
3.472GluAsp: 3.472 ± 0.34
3.635GluGlu: 3.635 ± 0.531
3.349GluPhe: 3.349 ± 0.405
3.268GluGly: 3.268 ± 0.378
1.348GluHis: 1.348 ± 0.221
4.534GluIle: 4.534 ± 0.387
3.676GluLys: 3.676 ± 0.465
5.922GluLeu: 5.922 ± 0.473
1.634GluMet: 1.634 ± 0.329
3.717GluAsn: 3.717 ± 0.328
2.532GluPro: 2.532 ± 0.369
2.492GluGln: 2.492 ± 0.344
3.145GluArg: 3.145 ± 0.355
3.513GluSer: 3.513 ± 0.316
3.758GluThr: 3.758 ± 0.47
4.003GluVal: 4.003 ± 0.418
1.389GluTrp: 1.389 ± 0.227
2.287GluTyr: 2.287 ± 0.304
0.0GluXaa: 0.0 ± 0.0
Phe
3.023PheAla: 3.023 ± 0.305
0.449PheCys: 0.449 ± 0.142
2.737PheAsp: 2.737 ± 0.3
2.328PheGlu: 2.328 ± 0.335
1.348PhePhe: 1.348 ± 0.232
2.737PheGly: 2.737 ± 0.342
0.694PheHis: 0.694 ± 0.169
2.532PheIle: 2.532 ± 0.277
2.369PheLys: 2.369 ± 0.284
2.328PheLeu: 2.328 ± 0.358
1.144PheMet: 1.144 ± 0.223
1.92PheAsn: 1.92 ± 0.24
1.838PhePro: 1.838 ± 0.257
1.266PheGln: 1.266 ± 0.214
2.246PheArg: 2.246 ± 0.286
2.206PheSer: 2.206 ± 0.287
3.676PheThr: 3.676 ± 0.328
2.124PheVal: 2.124 ± 0.387
0.408PheTrp: 0.408 ± 0.153
0.858PheTyr: 0.858 ± 0.244
0.0PheXaa: 0.0 ± 0.0
Gly
5.882GlyAla: 5.882 ± 1.012
0.449GlyCys: 0.449 ± 0.136
3.88GlyAsp: 3.88 ± 0.353
4.166GlyGlu: 4.166 ± 0.407
3.145GlyPhe: 3.145 ± 0.352
5.677GlyGly: 5.677 ± 0.854
1.47GlyHis: 1.47 ± 0.324
5.269GlyIle: 5.269 ± 0.467
5.432GlyLys: 5.432 ± 0.412
5.187GlyLeu: 5.187 ± 0.739
3.023GlyMet: 3.023 ± 0.5
3.839GlyAsn: 3.839 ± 0.406
2.573GlyPro: 2.573 ± 0.345
2.287GlyGln: 2.287 ± 0.272
3.104GlyArg: 3.104 ± 0.343
4.738GlySer: 4.738 ± 0.381
4.575GlyThr: 4.575 ± 0.546
4.697GlyVal: 4.697 ± 0.447
1.184GlyTrp: 1.184 ± 0.201
2.614GlyTyr: 2.614 ± 0.438
0.0GlyXaa: 0.0 ± 0.0
His
1.266HisAla: 1.266 ± 0.247
0.163HisCys: 0.163 ± 0.08
1.43HisAsp: 1.43 ± 0.284
1.062HisGlu: 1.062 ± 0.226
0.776HisPhe: 0.776 ± 0.159
1.389HisGly: 1.389 ± 0.26
0.449HisHis: 0.449 ± 0.148
1.144HisIle: 1.144 ± 0.222
0.817HisLys: 0.817 ± 0.189
1.021HisLeu: 1.021 ± 0.194
0.408HisMet: 0.408 ± 0.111
0.776HisAsn: 0.776 ± 0.189
0.654HisPro: 0.654 ± 0.155
0.572HisGln: 0.572 ± 0.17
1.266HisArg: 1.266 ± 0.305
1.062HisSer: 1.062 ± 0.18
1.103HisThr: 1.103 ± 0.237
1.266HisVal: 1.266 ± 0.267
0.286HisTrp: 0.286 ± 0.105
0.817HisTyr: 0.817 ± 0.208
0.0HisXaa: 0.0 ± 0.0
Ile
4.738IleAla: 4.738 ± 0.392
0.654IleCys: 0.654 ± 0.158
4.861IleAsp: 4.861 ± 0.394
4.82IleGlu: 4.82 ± 0.463
1.879IlePhe: 1.879 ± 0.252
4.942IleGly: 4.942 ± 0.4
0.98IleHis: 0.98 ± 0.218
3.227IleIle: 3.227 ± 0.362
4.003IleLys: 4.003 ± 0.4
4.003IleLeu: 4.003 ± 0.414
1.103IleMet: 1.103 ± 0.192
3.104IleAsn: 3.104 ± 0.303
3.268IlePro: 3.268 ± 0.341
1.552IleGln: 1.552 ± 0.239
2.941IleArg: 2.941 ± 0.329
4.166IleSer: 4.166 ± 0.373
4.697IleThr: 4.697 ± 0.431
4.33IleVal: 4.33 ± 0.316
0.776IleTrp: 0.776 ± 0.2
1.715IleTyr: 1.715 ± 0.244
0.0IleXaa: 0.0 ± 0.0
Lys
4.82LysAla: 4.82 ± 0.505
0.449LysCys: 0.449 ± 0.173
3.349LysAsp: 3.349 ± 0.538
4.125LysGlu: 4.125 ± 0.452
1.797LysPhe: 1.797 ± 0.292
3.717LysGly: 3.717 ± 0.513
1.103LysHis: 1.103 ± 0.227
3.839LysIle: 3.839 ± 0.395
4.615LysLys: 4.615 ± 0.612
4.861LysLeu: 4.861 ± 0.538
1.756LysMet: 1.756 ± 0.322
2.9LysAsn: 2.9 ± 0.388
2.655LysPro: 2.655 ± 0.389
2.41LysGln: 2.41 ± 0.315
3.227LysArg: 3.227 ± 0.483
3.594LysSer: 3.594 ± 0.4
3.472LysThr: 3.472 ± 0.398
4.534LysVal: 4.534 ± 0.458
1.021LysTrp: 1.021 ± 0.202
1.675LysTyr: 1.675 ± 0.286
0.0LysXaa: 0.0 ± 0.0
Leu
7.066LeuAla: 7.066 ± 0.689
0.939LeuCys: 0.939 ± 0.211
4.779LeuAsp: 4.779 ± 0.415
5.106LeuGlu: 5.106 ± 0.51
2.655LeuPhe: 2.655 ± 0.354
4.738LeuGly: 4.738 ± 0.587
0.817LeuHis: 0.817 ± 0.207
3.88LeuIle: 3.88 ± 0.423
4.044LeuLys: 4.044 ± 0.43
4.37LeuLeu: 4.37 ± 0.386
1.92LeuMet: 1.92 ± 0.3
3.758LeuAsn: 3.758 ± 0.443
3.799LeuPro: 3.799 ± 0.423
2.287LeuGln: 2.287 ± 0.337
3.268LeuArg: 3.268 ± 0.359
4.248LeuSer: 4.248 ± 0.43
4.942LeuThr: 4.942 ± 0.393
4.942LeuVal: 4.942 ± 0.47
0.939LeuTrp: 0.939 ± 0.177
2.369LeuTyr: 2.369 ± 0.356
0.0LeuXaa: 0.0 ± 0.0
Met
2.492MetAla: 2.492 ± 0.37
0.123MetCys: 0.123 ± 0.071
1.47MetAsp: 1.47 ± 0.211
1.307MetGlu: 1.307 ± 0.195
0.817MetPhe: 0.817 ± 0.22
2.492MetGly: 2.492 ± 0.234
0.531MetHis: 0.531 ± 0.174
1.47MetIle: 1.47 ± 0.203
1.797MetLys: 1.797 ± 0.208
1.838MetLeu: 1.838 ± 0.305
0.49MetMet: 0.49 ± 0.121
1.961MetAsn: 1.961 ± 0.325
1.389MetPro: 1.389 ± 0.277
1.103MetGln: 1.103 ± 0.207
1.389MetArg: 1.389 ± 0.22
2.246MetSer: 2.246 ± 0.294
1.47MetThr: 1.47 ± 0.251
1.634MetVal: 1.634 ± 0.236
0.245MetTrp: 0.245 ± 0.098
1.184MetTyr: 1.184 ± 0.241
0.0MetXaa: 0.0 ± 0.0
Asn
3.513AsnAla: 3.513 ± 0.391
0.531AsnCys: 0.531 ± 0.161
2.982AsnAsp: 2.982 ± 0.296
3.145AsnGlu: 3.145 ± 0.422
2.001AsnPhe: 2.001 ± 0.274
3.594AsnGly: 3.594 ± 0.359
0.817AsnHis: 0.817 ± 0.174
3.553AsnIle: 3.553 ± 0.429
3.717AsnLys: 3.717 ± 0.417
3.635AsnLeu: 3.635 ± 0.394
1.47AsnMet: 1.47 ± 0.245
2.859AsnAsn: 2.859 ± 0.318
3.349AsnPro: 3.349 ± 0.362
1.675AsnGln: 1.675 ± 0.264
2.492AsnArg: 2.492 ± 0.324
3.758AsnSer: 3.758 ± 0.352
3.349AsnThr: 3.349 ± 0.402
3.268AsnVal: 3.268 ± 0.344
0.776AsnTrp: 0.776 ± 0.163
2.001AsnTyr: 2.001 ± 0.266
0.0AsnXaa: 0.0 ± 0.0
Pro
5.228ProAla: 5.228 ± 0.723
0.327ProCys: 0.327 ± 0.138
3.227ProAsp: 3.227 ± 0.399
3.962ProGlu: 3.962 ± 0.403
1.593ProPhe: 1.593 ± 0.209
4.166ProGly: 4.166 ± 0.343
0.939ProHis: 0.939 ± 0.225
3.063ProIle: 3.063 ± 0.358
2.083ProLys: 2.083 ± 0.328
2.328ProLeu: 2.328 ± 0.224
1.511ProMet: 1.511 ± 0.244
2.859ProAsn: 2.859 ± 0.367
2.737ProPro: 2.737 ± 0.646
1.307ProGln: 1.307 ± 0.206
1.797ProArg: 1.797 ± 0.499
3.431ProSer: 3.431 ± 0.47
3.145ProThr: 3.145 ± 0.573
3.921ProVal: 3.921 ± 0.477
0.735ProTrp: 0.735 ± 0.179
1.756ProTyr: 1.756 ± 0.281
0.0ProXaa: 0.0 ± 0.0
Gln
3.88GlnAla: 3.88 ± 0.522
0.327GlnCys: 0.327 ± 0.127
1.715GlnAsp: 1.715 ± 0.224
2.206GlnGlu: 2.206 ± 0.313
1.511GlnPhe: 1.511 ± 0.251
2.206GlnGly: 2.206 ± 0.308
0.694GlnHis: 0.694 ± 0.203
2.41GlnIle: 2.41 ± 0.325
2.287GlnLys: 2.287 ± 0.312
2.859GlnLeu: 2.859 ± 0.335
1.062GlnMet: 1.062 ± 0.212
1.389GlnAsn: 1.389 ± 0.225
1.144GlnPro: 1.144 ± 0.286
1.266GlnGln: 1.266 ± 0.368
1.43GlnArg: 1.43 ± 0.225
2.41GlnSer: 2.41 ± 0.303
2.083GlnThr: 2.083 ± 0.361
2.042GlnVal: 2.042 ± 0.284
0.817GlnTrp: 0.817 ± 0.15
1.225GlnTyr: 1.225 ± 0.209
0.0GlnXaa: 0.0 ± 0.0
Arg
4.37ArgAla: 4.37 ± 0.703
0.531ArgCys: 0.531 ± 0.153
3.268ArgAsp: 3.268 ± 0.39
2.287ArgGlu: 2.287 ± 0.299
1.879ArgPhe: 1.879 ± 0.254
3.104ArgGly: 3.104 ± 0.337
1.103ArgHis: 1.103 ± 0.211
3.268ArgIle: 3.268 ± 0.444
3.145ArgLys: 3.145 ± 0.339
3.513ArgLeu: 3.513 ± 0.417
1.797ArgMet: 1.797 ± 0.286
2.042ArgAsn: 2.042 ± 0.223
2.451ArgPro: 2.451 ± 0.338
1.348ArgGln: 1.348 ± 0.188
2.614ArgArg: 2.614 ± 0.356
2.696ArgSer: 2.696 ± 0.353
2.655ArgThr: 2.655 ± 0.328
2.696ArgVal: 2.696 ± 0.391
1.103ArgTrp: 1.103 ± 0.166
2.042ArgTyr: 2.042 ± 0.349
0.0ArgXaa: 0.0 ± 0.0
Ser
4.983SerAla: 4.983 ± 0.615
0.613SerCys: 0.613 ± 0.174
4.452SerAsp: 4.452 ± 0.54
3.88SerGlu: 3.88 ± 0.577
2.451SerPhe: 2.451 ± 0.342
5.514SerGly: 5.514 ± 0.561
0.735SerHis: 0.735 ± 0.186
3.513SerIle: 3.513 ± 0.36
3.513SerLys: 3.513 ± 0.458
4.697SerLeu: 4.697 ± 0.403
2.246SerMet: 2.246 ± 0.282
3.594SerAsn: 3.594 ± 0.414
3.349SerPro: 3.349 ± 0.371
2.042SerGln: 2.042 ± 0.226
2.696SerArg: 2.696 ± 0.34
3.349SerSer: 3.349 ± 0.522
4.003SerThr: 4.003 ± 0.532
4.289SerVal: 4.289 ± 0.543
0.899SerTrp: 0.899 ± 0.175
1.715SerTyr: 1.715 ± 0.291
0.0SerXaa: 0.0 ± 0.0
Thr
6.331ThrAla: 6.331 ± 0.881
0.572ThrCys: 0.572 ± 0.166
5.106ThrAsp: 5.106 ± 0.483
3.268ThrGlu: 3.268 ± 0.407
2.532ThrPhe: 2.532 ± 0.296
5.841ThrGly: 5.841 ± 0.668
1.184ThrHis: 1.184 ± 0.225
3.268ThrIle: 3.268 ± 0.372
3.472ThrLys: 3.472 ± 0.348
4.166ThrLeu: 4.166 ± 0.381
1.144ThrMet: 1.144 ± 0.24
3.431ThrAsn: 3.431 ± 0.39
3.635ThrPro: 3.635 ± 0.546
2.818ThrGln: 2.818 ± 0.3
2.696ThrArg: 2.696 ± 0.329
3.962ThrSer: 3.962 ± 0.545
3.186ThrThr: 3.186 ± 0.411
4.452ThrVal: 4.452 ± 0.647
0.694ThrTrp: 0.694 ± 0.16
2.451ThrTyr: 2.451 ± 0.308
0.0ThrXaa: 0.0 ± 0.0
Val
5.065ValAla: 5.065 ± 0.477
0.939ValCys: 0.939 ± 0.217
5.065ValAsp: 5.065 ± 0.62
4.534ValGlu: 4.534 ± 0.363
3.063ValPhe: 3.063 ± 0.457
4.697ValGly: 4.697 ± 0.555
1.021ValHis: 1.021 ± 0.216
4.248ValIle: 4.248 ± 0.476
2.941ValLys: 2.941 ± 0.359
4.044ValLeu: 4.044 ± 0.413
1.593ValMet: 1.593 ± 0.219
3.717ValAsn: 3.717 ± 0.366
3.472ValPro: 3.472 ± 0.349
2.165ValGln: 2.165 ± 0.268
3.472ValArg: 3.472 ± 0.332
4.003ValSer: 4.003 ± 0.513
4.861ValThr: 4.861 ± 0.446
3.553ValVal: 3.553 ± 0.46
0.899ValTrp: 0.899 ± 0.179
2.042ValTyr: 2.042 ± 0.277
0.0ValXaa: 0.0 ± 0.0
Trp
1.348TrpAla: 1.348 ± 0.292
0.163TrpCys: 0.163 ± 0.09
0.98TrpAsp: 0.98 ± 0.187
0.899TrpGlu: 0.899 ± 0.226
0.49TrpPhe: 0.49 ± 0.135
0.776TrpGly: 0.776 ± 0.143
0.327TrpHis: 0.327 ± 0.102
0.939TrpIle: 0.939 ± 0.182
0.939TrpLys: 0.939 ± 0.168
1.43TrpLeu: 1.43 ± 0.237
0.368TrpMet: 0.368 ± 0.116
1.184TrpAsn: 1.184 ± 0.185
0.327TrpPro: 0.327 ± 0.128
0.899TrpGln: 0.899 ± 0.173
0.98TrpArg: 0.98 ± 0.191
0.858TrpSer: 0.858 ± 0.24
0.694TrpThr: 0.694 ± 0.173
0.899TrpVal: 0.899 ± 0.174
0.204TrpTrp: 0.204 ± 0.122
0.776TrpTyr: 0.776 ± 0.202
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.532TyrAla: 2.532 ± 0.376
0.572TyrCys: 0.572 ± 0.153
2.328TyrAsp: 2.328 ± 0.353
1.879TyrGlu: 1.879 ± 0.379
0.98TyrPhe: 0.98 ± 0.169
2.696TyrGly: 2.696 ± 0.359
0.735TyrHis: 0.735 ± 0.161
1.756TyrIle: 1.756 ± 0.239
1.879TyrLys: 1.879 ± 0.344
2.818TyrLeu: 2.818 ± 0.491
0.572TyrMet: 0.572 ± 0.124
1.838TyrAsn: 1.838 ± 0.279
1.511TyrPro: 1.511 ± 0.256
1.675TyrGln: 1.675 ± 0.245
1.961TyrArg: 1.961 ± 0.333
2.451TyrSer: 2.451 ± 0.434
2.287TyrThr: 2.287 ± 0.36
2.328TyrVal: 2.328 ± 0.344
0.327TyrTrp: 0.327 ± 0.121
1.062TyrTyr: 1.062 ± 0.201
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 117 proteins (24484 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski