Pseudonocardia asaccharolytica DSM 44247 = NBRC 16224

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Pseudonocardia; Pseudonocardia asaccharolytica

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4883 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A511CUP0|A0A511CUP0_9PSEU 50S ribosomal protein L7/L12 OS=Pseudonocardia asaccharolytica DSM 44247 = NBRC 16224 OX=1123024 GN=rplL PE=3 SV=1
MM1 pKa = 7.45SPDD4 pKa = 4.29ALPPDD9 pKa = 4.56PFAGDD14 pKa = 3.48PHH16 pKa = 7.93DD17 pKa = 4.76PALSLDD23 pKa = 4.2GPDD26 pKa = 3.17ATEE29 pKa = 4.29PEE31 pKa = 4.39VLSEE35 pKa = 4.01QEE37 pKa = 3.48RR38 pKa = 11.84DD39 pKa = 3.74EE40 pKa = 4.41IVADD44 pKa = 3.98LADD47 pKa = 3.94LAVYY51 pKa = 9.68QALLEE56 pKa = 4.28TRR58 pKa = 11.84GVRR61 pKa = 11.84GIVVDD66 pKa = 4.84CGDD69 pKa = 3.76CGEE72 pKa = 4.22QHH74 pKa = 4.97FHH76 pKa = 5.74EE77 pKa = 4.93WSLLRR82 pKa = 11.84ASLQQLLDD90 pKa = 3.76EE91 pKa = 4.95GQMRR95 pKa = 11.84PHH97 pKa = 6.8EE98 pKa = 4.45PAFDD102 pKa = 5.17PDD104 pKa = 3.67PADD107 pKa = 3.48YY108 pKa = 9.58VTWEE112 pKa = 4.03YY113 pKa = 11.24CRR115 pKa = 11.84GYY117 pKa = 11.37ADD119 pKa = 5.23ALLSDD124 pKa = 4.14SS125 pKa = 4.25

Molecular weight: 13.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A511D4E9|A0A511D4E9_9PSEU Uncharacterized protein OS=Pseudonocardia asaccharolytica DSM 44247 = NBRC 16224 OX=1123024 GN=PA7_31900 PE=4 SV=1
MM1 pKa = 7.7GSPGTGVLRR10 pKa = 11.84VVRR13 pKa = 11.84ATAVTATVVGFAAAAHH29 pKa = 5.57TLGGASVPGPTLAAGSALVLLIVSAVSGQRR59 pKa = 11.84FSGAALLALLAVGQFALHH77 pKa = 6.16HH78 pKa = 6.29VFTIGAPAHH87 pKa = 6.66AMGQGSASPAMLVAHH102 pKa = 6.1VVATVVSTGVLLRR115 pKa = 11.84GEE117 pKa = 4.11RR118 pKa = 11.84ALWAMWSWLWRR129 pKa = 11.84LLPTAVTLWLPVPARR144 pKa = 11.84PPRR147 pKa = 11.84RR148 pKa = 11.84WTRR151 pKa = 11.84RR152 pKa = 11.84APRR155 pKa = 11.84PRR157 pKa = 11.84LVLLLTAARR166 pKa = 11.84RR167 pKa = 11.84GPPAPARR174 pKa = 11.84PLSAASS180 pKa = 3.36

Molecular weight: 18.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4883

0

4883

1461661

39

2138

299.3

32.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.992 ± 0.058

0.871 ± 0.011

5.954 ± 0.029

5.565 ± 0.034

2.644 ± 0.021

9.472 ± 0.031

2.273 ± 0.015

3.51 ± 0.022

1.527 ± 0.022

10.528 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.743 ± 0.012

1.601 ± 0.016

6.299 ± 0.03

2.628 ± 0.019

8.756 ± 0.04

4.651 ± 0.022

5.656 ± 0.026

8.997 ± 0.039

1.465 ± 0.015

1.867 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski