Amino acid dipepetide frequency for ANME-2 cluster archaeon

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.551AlaAla: 6.551 ± 0.132
1.083AlaCys: 1.083 ± 0.042
5.221AlaAsp: 5.221 ± 0.078
5.139AlaGlu: 5.139 ± 0.082
2.636AlaPhe: 2.636 ± 0.061
6.452AlaGly: 6.452 ± 0.101
1.454AlaHis: 1.454 ± 0.043
6.123AlaIle: 6.123 ± 0.095
3.517AlaLys: 3.517 ± 0.064
6.604AlaLeu: 6.604 ± 0.122
2.208AlaMet: 2.208 ± 0.053
2.598AlaAsn: 2.598 ± 0.055
2.573AlaPro: 2.573 ± 0.054
1.549AlaGln: 1.549 ± 0.042
3.899AlaArg: 3.899 ± 0.071
4.859AlaSer: 4.859 ± 0.076
4.338AlaThr: 4.338 ± 0.079
5.917AlaVal: 5.917 ± 0.103
0.751AlaTrp: 0.751 ± 0.027
2.364AlaTyr: 2.364 ± 0.056
0.0AlaXaa: 0.0 ± 0.0
Cys
1.037CysAla: 1.037 ± 0.039
0.238CysCys: 0.238 ± 0.015
0.95CysAsp: 0.95 ± 0.033
0.941CysGlu: 0.941 ± 0.032
0.414CysPhe: 0.414 ± 0.022
1.683CysGly: 1.683 ± 0.054
0.492CysHis: 0.492 ± 0.063
0.974CysIle: 0.974 ± 0.037
0.682CysLys: 0.682 ± 0.032
0.952CysLeu: 0.952 ± 0.034
0.355CysMet: 0.355 ± 0.019
0.561CysAsn: 0.561 ± 0.025
0.772CysPro: 0.772 ± 0.04
0.311CysGln: 0.311 ± 0.018
0.653CysArg: 0.653 ± 0.026
0.818CysSer: 0.818 ± 0.034
0.812CysThr: 0.812 ± 0.033
0.965CysVal: 0.965 ± 0.034
0.167CysTrp: 0.167 ± 0.014
0.455CysTyr: 0.455 ± 0.024
0.0CysXaa: 0.0 ± 0.0
Asp
6.056AspAla: 6.056 ± 0.081
0.998AspCys: 0.998 ± 0.042
4.227AspAsp: 4.227 ± 0.09
4.873AspGlu: 4.873 ± 0.078
2.488AspPhe: 2.488 ± 0.052
5.571AspGly: 5.571 ± 0.121
1.303AspHis: 1.303 ± 0.04
5.015AspIle: 5.015 ± 0.09
2.591AspLys: 2.591 ± 0.065
6.133AspLeu: 6.133 ± 0.087
1.66AspMet: 1.66 ± 0.039
2.277AspAsn: 2.277 ± 0.077
3.17AspPro: 3.17 ± 0.065
1.422AspGln: 1.422 ± 0.045
3.582AspArg: 3.582 ± 0.07
3.595AspSer: 3.595 ± 0.074
3.407AspThr: 3.407 ± 0.075
5.441AspVal: 5.441 ± 0.084
0.832AspTrp: 0.832 ± 0.036
2.408AspTyr: 2.408 ± 0.059
0.0AspXaa: 0.0 ± 0.0
Glu
4.286GluAla: 4.286 ± 0.083
0.86GluCys: 0.86 ± 0.033
3.988GluAsp: 3.988 ± 0.075
5.348GluGlu: 5.348 ± 0.123
2.742GluPhe: 2.742 ± 0.054
4.274GluGly: 4.274 ± 0.073
1.438GluHis: 1.438 ± 0.044
6.358GluIle: 6.358 ± 0.098
4.171GluLys: 4.171 ± 0.1
7.005GluLeu: 7.005 ± 0.106
2.132GluMet: 2.132 ± 0.05
2.811GluAsn: 2.811 ± 0.053
2.362GluPro: 2.362 ± 0.051
1.812GluGln: 1.812 ± 0.046
3.96GluArg: 3.96 ± 0.086
4.024GluSer: 4.024 ± 0.078
3.989GluThr: 3.989 ± 0.067
4.773GluVal: 4.773 ± 0.075
0.832GluTrp: 0.832 ± 0.028
2.68GluTyr: 2.68 ± 0.057
0.0GluXaa: 0.0 ± 0.0
Phe
2.705PheAla: 2.705 ± 0.058
0.539PheCys: 0.539 ± 0.026
2.622PheAsp: 2.622 ± 0.057
2.79PheGlu: 2.79 ± 0.061
1.412PhePhe: 1.412 ± 0.047
3.157PheGly: 3.157 ± 0.066
0.729PheHis: 0.729 ± 0.025
2.567PheIle: 2.567 ± 0.065
1.818PheLys: 1.818 ± 0.053
3.088PheLeu: 3.088 ± 0.085
0.863PheMet: 0.863 ± 0.031
1.439PheAsn: 1.439 ± 0.053
1.308PhePro: 1.308 ± 0.037
0.793PheGln: 0.793 ± 0.031
1.725PheArg: 1.725 ± 0.049
2.51PheSer: 2.51 ± 0.06
2.084PheThr: 2.084 ± 0.05
2.815PheVal: 2.815 ± 0.054
0.442PheTrp: 0.442 ± 0.027
1.318PheTyr: 1.318 ± 0.041
0.0PheXaa: 0.0 ± 0.0
Gly
6.088GlyAla: 6.088 ± 0.109
1.335GlyCys: 1.335 ± 0.044
5.495GlyAsp: 5.495 ± 0.119
4.942GlyGlu: 4.942 ± 0.08
2.894GlyPhe: 2.894 ± 0.055
6.084GlyGly: 6.084 ± 0.128
1.33GlyHis: 1.33 ± 0.039
6.506GlyIle: 6.506 ± 0.109
4.149GlyLys: 4.149 ± 0.081
5.488GlyLeu: 5.488 ± 0.089
2.447GlyMet: 2.447 ± 0.066
3.036GlyAsn: 3.036 ± 0.099
1.704GlyPro: 1.704 ± 0.051
1.413GlyGln: 1.413 ± 0.04
4.149GlyArg: 4.149 ± 0.084
5.099GlySer: 5.099 ± 0.094
4.924GlyThr: 4.924 ± 0.076
5.91GlyVal: 5.91 ± 0.098
1.049GlyTrp: 1.049 ± 0.035
3.123GlyTyr: 3.123 ± 0.063
0.0GlyXaa: 0.0 ± 0.0
His
1.596HisAla: 1.596 ± 0.039
0.377HisCys: 0.377 ± 0.02
1.388HisAsp: 1.388 ± 0.04
1.283HisGlu: 1.283 ± 0.044
0.713HisPhe: 0.713 ± 0.03
1.673HisGly: 1.673 ± 0.047
0.575HisHis: 0.575 ± 0.033
1.567HisIle: 1.567 ± 0.04
0.858HisLys: 0.858 ± 0.029
2.008HisLeu: 2.008 ± 0.05
0.217HisMet: 0.217 ± 0.015
0.853HisAsn: 0.853 ± 0.037
1.223HisPro: 1.223 ± 0.038
0.535HisGln: 0.535 ± 0.024
1.118HisArg: 1.118 ± 0.034
1.126HisSer: 1.126 ± 0.035
1.23HisThr: 1.23 ± 0.039
1.342HisVal: 1.342 ± 0.038
0.26HisTrp: 0.26 ± 0.014
0.662HisTyr: 0.662 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
6.382IleAla: 6.382 ± 0.107
1.041IleCys: 1.041 ± 0.035
5.408IleAsp: 5.408 ± 0.086
6.014IleGlu: 6.014 ± 0.1
2.808IlePhe: 2.808 ± 0.064
6.039IleGly: 6.039 ± 0.096
1.486IleHis: 1.486 ± 0.046
4.876IleIle: 4.876 ± 0.09
3.764IleLys: 3.764 ± 0.063
6.404IleLeu: 6.404 ± 0.111
1.576IleMet: 1.576 ± 0.048
2.68IleAsn: 2.68 ± 0.049
3.669IlePro: 3.669 ± 0.059
1.544IleGln: 1.544 ± 0.036
4.455IleArg: 4.455 ± 0.072
5.182IleSer: 5.182 ± 0.075
5.036IleThr: 5.036 ± 0.068
5.455IleVal: 5.455 ± 0.087
0.722IleTrp: 0.722 ± 0.032
2.526IleTyr: 2.526 ± 0.058
0.0IleXaa: 0.0 ± 0.0
Lys
3.14LysAla: 3.14 ± 0.073
0.554LysCys: 0.554 ± 0.026
3.091LysAsp: 3.091 ± 0.059
4.284LysGlu: 4.284 ± 0.09
1.444LysPhe: 1.444 ± 0.046
3.223LysGly: 3.223 ± 0.059
1.079LysHis: 1.079 ± 0.036
4.131LysIle: 4.131 ± 0.072
3.41LysLys: 3.41 ± 0.084
4.134LysLeu: 4.134 ± 0.078
1.401LysMet: 1.401 ± 0.038
2.328LysAsn: 2.328 ± 0.054
2.073LysPro: 2.073 ± 0.051
1.427LysGln: 1.427 ± 0.043
3.222LysArg: 3.222 ± 0.074
3.074LysSer: 3.074 ± 0.06
3.096LysThr: 3.096 ± 0.062
3.144LysVal: 3.144 ± 0.061
0.591LysTrp: 0.591 ± 0.03
1.843LysTyr: 1.843 ± 0.047
0.0LysXaa: 0.0 ± 0.0
Leu
6.85LeuAla: 6.85 ± 0.109
1.218LeuCys: 1.218 ± 0.04
5.724LeuAsp: 5.724 ± 0.083
5.427LeuGlu: 5.427 ± 0.089
3.391LeuPhe: 3.391 ± 0.078
5.846LeuGly: 5.846 ± 0.11
1.833LeuHis: 1.833 ± 0.043
6.361LeuIle: 6.361 ± 0.108
5.048LeuLys: 5.048 ± 0.095
7.909LeuLeu: 7.909 ± 0.125
2.342LeuMet: 2.342 ± 0.052
3.412LeuAsn: 3.412 ± 0.066
3.782LeuPro: 3.782 ± 0.072
2.287LeuGln: 2.287 ± 0.054
4.444LeuArg: 4.444 ± 0.085
5.998LeuSer: 5.998 ± 0.091
4.959LeuThr: 4.959 ± 0.089
5.883LeuVal: 5.883 ± 0.093
0.943LeuTrp: 0.943 ± 0.039
2.559LeuTyr: 2.559 ± 0.06
0.0LeuXaa: 0.0 ± 0.0
Met
1.844MetAla: 1.844 ± 0.049
0.298MetCys: 0.298 ± 0.017
1.738MetAsp: 1.738 ± 0.038
1.744MetGlu: 1.744 ± 0.044
0.796MetPhe: 0.796 ± 0.029
1.826MetGly: 1.826 ± 0.049
0.601MetHis: 0.601 ± 0.024
2.122MetIle: 2.122 ± 0.056
1.652MetLys: 1.652 ± 0.043
2.314MetLeu: 2.314 ± 0.058
0.748MetMet: 0.748 ± 0.03
1.236MetAsn: 1.236 ± 0.04
1.176MetPro: 1.176 ± 0.038
0.772MetGln: 0.772 ± 0.027
1.509MetArg: 1.509 ± 0.043
1.454MetSer: 1.454 ± 0.038
1.554MetThr: 1.554 ± 0.044
1.95MetVal: 1.95 ± 0.043
0.207MetTrp: 0.207 ± 0.015
0.773MetTyr: 0.773 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
2.896AsnAla: 2.896 ± 0.06
0.637AsnCys: 0.637 ± 0.034
2.184AsnAsp: 2.184 ± 0.065
2.394AsnGlu: 2.394 ± 0.053
1.45AsnPhe: 1.45 ± 0.042
2.858AsnGly: 2.858 ± 0.085
0.769AsnHis: 0.769 ± 0.034
3.361AsnIle: 3.361 ± 0.067
1.78AsnLys: 1.78 ± 0.049
3.545AsnLeu: 3.545 ± 0.072
0.921AsnMet: 0.921 ± 0.034
2.271AsnAsn: 2.271 ± 0.171
2.073AsnPro: 2.073 ± 0.053
0.95AsnGln: 0.95 ± 0.041
2.195AsnArg: 2.195 ± 0.05
2.237AsnSer: 2.237 ± 0.067
2.445AsnThr: 2.445 ± 0.092
2.966AsnVal: 2.966 ± 0.076
0.572AsnTrp: 0.572 ± 0.032
1.38AsnTyr: 1.38 ± 0.046
0.0AsnXaa: 0.0 ± 0.0
Pro
3.228ProAla: 3.228 ± 0.068
0.5ProCys: 0.5 ± 0.025
3.754ProAsp: 3.754 ± 0.079
3.544ProGlu: 3.544 ± 0.069
1.454ProPhe: 1.454 ± 0.037
3.022ProGly: 3.022 ± 0.067
0.98ProHis: 0.98 ± 0.036
2.721ProIle: 2.721 ± 0.054
1.813ProLys: 1.813 ± 0.045
3.106ProLeu: 3.106 ± 0.062
0.891ProMet: 0.891 ± 0.032
1.347ProAsn: 1.347 ± 0.041
1.833ProPro: 1.833 ± 0.052
0.93ProGln: 0.93 ± 0.028
1.601ProArg: 1.601 ± 0.042
2.313ProSer: 2.313 ± 0.056
2.211ProThr: 2.211 ± 0.058
3.461ProVal: 3.461 ± 0.066
0.37ProTrp: 0.37 ± 0.021
1.358ProTyr: 1.358 ± 0.037
0.0ProXaa: 0.0 ± 0.0
Gln
1.691GlnAla: 1.691 ± 0.05
0.287GlnCys: 0.287 ± 0.019
1.188GlnAsp: 1.188 ± 0.042
1.619GlnGlu: 1.619 ± 0.05
0.829GlnPhe: 0.829 ± 0.028
1.397GlnGly: 1.397 ± 0.043
0.458GlnHis: 0.458 ± 0.025
1.971GlnIle: 1.971 ± 0.052
1.351GlnLys: 1.351 ± 0.044
2.051GlnLeu: 2.051 ± 0.052
0.725GlnMet: 0.725 ± 0.03
1.016GlnAsn: 1.016 ± 0.04
0.874GlnPro: 0.874 ± 0.031
0.798GlnGln: 0.798 ± 0.036
1.377GlnArg: 1.377 ± 0.051
1.457GlnSer: 1.457 ± 0.04
1.55GlnThr: 1.55 ± 0.043
1.637GlnVal: 1.637 ± 0.04
0.294GlnTrp: 0.294 ± 0.018
0.852GlnTyr: 0.852 ± 0.032
0.0GlnXaa: 0.0 ± 0.0
Arg
3.625ArgAla: 3.625 ± 0.073
0.823ArgCys: 0.823 ± 0.03
3.526ArgAsp: 3.526 ± 0.065
3.861ArgGlu: 3.861 ± 0.078
2.245ArgPhe: 2.245 ± 0.056
3.753ArgGly: 3.753 ± 0.067
1.054ArgHis: 1.054 ± 0.037
4.582ArgIle: 4.582 ± 0.077
3.103ArgLys: 3.103 ± 0.072
4.551ArgLeu: 4.551 ± 0.075
1.766ArgMet: 1.766 ± 0.046
1.995ArgAsn: 1.995 ± 0.048
1.477ArgPro: 1.477 ± 0.045
1.295ArgGln: 1.295 ± 0.043
3.112ArgArg: 3.112 ± 0.074
3.123ArgSer: 3.123 ± 0.063
2.763ArgThr: 2.763 ± 0.06
3.817ArgVal: 3.817 ± 0.074
0.65ArgTrp: 0.65 ± 0.028
2.195ArgTyr: 2.195 ± 0.049
0.0ArgXaa: 0.0 ± 0.0
Ser
4.638SerAla: 4.638 ± 0.08
0.845SerCys: 0.845 ± 0.032
4.697SerAsp: 4.697 ± 0.081
4.534SerGlu: 4.534 ± 0.069
2.177SerPhe: 2.177 ± 0.055
6.156SerGly: 6.156 ± 0.09
1.167SerHis: 1.167 ± 0.035
4.597SerIle: 4.597 ± 0.087
2.845SerLys: 2.845 ± 0.063
4.987SerLeu: 4.987 ± 0.085
1.586SerMet: 1.586 ± 0.042
2.633SerAsn: 2.633 ± 0.113
2.439SerPro: 2.439 ± 0.06
1.402SerGln: 1.402 ± 0.038
3.203SerArg: 3.203 ± 0.061
3.973SerSer: 3.973 ± 0.113
3.281SerThr: 3.281 ± 0.063
4.568SerVal: 4.568 ± 0.085
0.616SerTrp: 0.616 ± 0.03
1.975SerTyr: 1.975 ± 0.053
0.0SerXaa: 0.0 ± 0.0
Thr
4.421ThrAla: 4.421 ± 0.092
0.751ThrCys: 0.751 ± 0.03
3.826ThrAsp: 3.826 ± 0.076
3.656ThrGlu: 3.656 ± 0.057
2.088ThrPhe: 2.088 ± 0.052
5.407ThrGly: 5.407 ± 0.093
1.184ThrHis: 1.184 ± 0.04
4.812ThrIle: 4.812 ± 0.081
2.586ThrLys: 2.586 ± 0.058
5.091ThrLeu: 5.091 ± 0.087
1.373ThrMet: 1.373 ± 0.045
2.239ThrAsn: 2.239 ± 0.076
3.007ThrPro: 3.007 ± 0.063
1.14ThrGln: 1.14 ± 0.037
2.597ThrArg: 2.597 ± 0.056
3.581ThrSer: 3.581 ± 0.077
3.635ThrThr: 3.635 ± 0.085
4.785ThrVal: 4.785 ± 0.086
0.599ThrTrp: 0.599 ± 0.03
1.971ThrTyr: 1.971 ± 0.056
0.0ThrXaa: 0.0 ± 0.0
Val
5.598ValAla: 5.598 ± 0.092
1.218ValCys: 1.218 ± 0.041
4.72ValAsp: 4.72 ± 0.075
4.63ValGlu: 4.63 ± 0.07
2.96ValPhe: 2.96 ± 0.059
5.051ValGly: 5.051 ± 0.085
1.541ValHis: 1.541 ± 0.048
5.368ValIle: 5.368 ± 0.086
3.405ValLys: 3.405 ± 0.06
6.673ValLeu: 6.673 ± 0.101
1.993ValMet: 1.993 ± 0.052
2.897ValAsn: 2.897 ± 0.068
3.09ValPro: 3.09 ± 0.065
1.645ValGln: 1.645 ± 0.05
4.027ValArg: 4.027 ± 0.073
5.012ValSer: 5.012 ± 0.076
4.733ValThr: 4.733 ± 0.093
5.936ValVal: 5.936 ± 0.088
0.832ValTrp: 0.832 ± 0.036
2.516ValTyr: 2.516 ± 0.061
0.0ValXaa: 0.0 ± 0.0
Trp
0.749TrpAla: 0.749 ± 0.032
0.181TrpCys: 0.181 ± 0.015
0.915TrpAsp: 0.915 ± 0.037
0.616TrpGlu: 0.616 ± 0.024
0.451TrpPhe: 0.451 ± 0.025
0.824TrpGly: 0.824 ± 0.031
0.279TrpHis: 0.279 ± 0.018
0.869TrpIle: 0.869 ± 0.033
0.646TrpLys: 0.646 ± 0.029
0.908TrpLeu: 0.908 ± 0.033
0.353TrpMet: 0.353 ± 0.022
0.68TrpAsn: 0.68 ± 0.038
0.183TrpPro: 0.183 ± 0.016
0.374TrpGln: 0.374 ± 0.022
0.586TrpArg: 0.586 ± 0.025
0.681TrpSer: 0.681 ± 0.032
0.596TrpThr: 0.596 ± 0.028
0.822TrpVal: 0.822 ± 0.037
0.229TrpTrp: 0.229 ± 0.017
0.496TrpTyr: 0.496 ± 0.028
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.454TyrAla: 2.454 ± 0.056
0.45TyrCys: 0.45 ± 0.023
2.389TyrAsp: 2.389 ± 0.07
2.208TyrGlu: 2.208 ± 0.054
1.333TyrPhe: 1.333 ± 0.037
2.868TyrGly: 2.868 ± 0.067
0.852TyrHis: 0.852 ± 0.033
2.187TyrIle: 2.187 ± 0.05
1.503TyrLys: 1.503 ± 0.037
3.262TyrLeu: 3.262 ± 0.062
0.759TyrMet: 0.759 ± 0.028
1.575TyrAsn: 1.575 ± 0.051
1.648TyrPro: 1.648 ± 0.042
1.025TyrGln: 1.025 ± 0.035
1.9TyrArg: 1.9 ± 0.045
2.202TyrSer: 2.202 ± 0.062
2.106TyrThr: 2.106 ± 0.056
2.256TyrVal: 2.256 ± 0.054
0.478TyrTrp: 0.478 ± 0.025
1.393TyrTyr: 1.393 ± 0.044
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3377 proteins (955486 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski