Escherichia phage ECBP1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Enquatrovirinae; Gamaleyavirus; Escherichia virus ECBP1

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J9RVQ5|J9RVQ5_9CAUD Uncharacterized protein OS=Escherichia phage ECBP1 OX=1604356 GN=ECBP1_0061 PE=4 SV=1
MM1 pKa = 7.14ATVSKK6 pKa = 10.28EE7 pKa = 3.9AIEE10 pKa = 4.5AKK12 pKa = 9.96IKK14 pKa = 10.23SVYY17 pKa = 9.63YY18 pKa = 10.27FNGADD23 pKa = 3.32AVKK26 pKa = 10.42SAFVDD31 pKa = 5.21PSALPADD38 pKa = 4.27DD39 pKa = 4.65LANLGLVTYY48 pKa = 9.26CVIILEE54 pKa = 4.37NNFKK58 pKa = 11.02VEE60 pKa = 4.2GVSACVDD67 pKa = 3.14PTIYY71 pKa = 10.55DD72 pKa = 3.69EE73 pKa = 4.34QLGRR77 pKa = 11.84QYY79 pKa = 11.46AYY81 pKa = 10.1EE82 pKa = 3.92NAFNKK87 pKa = 9.47IWEE90 pKa = 4.31LEE92 pKa = 4.3GYY94 pKa = 10.14LLRR97 pKa = 11.84QALHH101 pKa = 6.28EE102 pKa = 4.37KK103 pKa = 10.79EE104 pKa = 4.16EE105 pKa = 4.19TAKK108 pKa = 11.03ALASFAEE115 pKa = 4.27NNTCDD120 pKa = 4.75GGGCTII126 pKa = 4.85

Molecular weight: 13.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J9RVR7|J9RVR7_9CAUD Uncharacterized protein OS=Escherichia phage ECBP1 OX=1604356 GN=ECBP1_0077 PE=4 SV=1
MM1 pKa = 7.61VDD3 pKa = 4.65GIQLGMFNISGQVITLTAVSVRR25 pKa = 11.84VMQTVTLYY33 pKa = 10.71KK34 pKa = 10.17KK35 pKa = 10.3RR36 pKa = 11.84PKK38 pKa = 10.47LGLSFNN44 pKa = 4.21

Molecular weight: 4.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

21275

42

3570

259.5

28.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.597 ± 0.6

0.846 ± 0.158

5.734 ± 0.164

6.599 ± 0.266

3.638 ± 0.183

6.618 ± 0.255

1.758 ± 0.16

5.716 ± 0.234

6.599 ± 0.308

8.315 ± 0.219

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.957 ± 0.118

5.763 ± 0.148

4.169 ± 0.151

4.188 ± 0.277

4.235 ± 0.186

6.12 ± 0.234

6.454 ± 0.298

6.867 ± 0.248

1.142 ± 0.103

3.685 ± 0.253
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski