Listeria phage P40

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B6D7I9|B6D7I9_9CAUD Gp16 OS=Listeria phage P40 OX=560178 PE=4 SV=1
MM1 pKa = 7.46IPKK4 pKa = 10.3KK5 pKa = 9.82LTLINGRR12 pKa = 11.84GDD14 pKa = 3.54ILPLNDD20 pKa = 3.89NGSTGLFVLEE30 pKa = 4.11WSGLGVNINFDD41 pKa = 3.69LDD43 pKa = 3.55KK44 pKa = 10.8YY45 pKa = 10.29QASQIVKK52 pKa = 10.04NRR54 pKa = 11.84TLDD57 pKa = 3.34MQNFVVNIKK66 pKa = 10.3IGLNYY71 pKa = 10.39SATTPRR77 pKa = 11.84QIYY80 pKa = 10.23AGLIQFLNHH89 pKa = 6.02TPYY92 pKa = 10.55TLQSEE97 pKa = 4.56DD98 pKa = 3.84DD99 pKa = 3.6AGTYY103 pKa = 10.3LRR105 pKa = 11.84DD106 pKa = 3.6VQLQTMTLTDD116 pKa = 3.98LKK118 pKa = 10.29EE119 pKa = 4.0GQIFEE124 pKa = 4.36EE125 pKa = 4.35TLEE128 pKa = 4.64FICTSLWYY136 pKa = 10.73SEE138 pKa = 5.7DD139 pKa = 3.18IYY141 pKa = 10.97EE142 pKa = 4.22YY143 pKa = 10.36TPQDD147 pKa = 3.48LSIPADD153 pKa = 3.41TGKK156 pKa = 10.24VFDD159 pKa = 4.91KK160 pKa = 11.52NEE162 pKa = 3.92STTVKK167 pKa = 10.47NQGGYY172 pKa = 10.9GYY174 pKa = 10.86LLPTDD179 pKa = 4.27VNIAPYY185 pKa = 10.38EE186 pKa = 3.96PVGNGAITPDD196 pKa = 3.96MYY198 pKa = 11.26EE199 pKa = 4.47LGSPTITGAFLDD211 pKa = 3.69IDD213 pKa = 4.01EE214 pKa = 5.56LNVTFQAKK222 pKa = 10.05VYY224 pKa = 10.99LNGTLLFTGGTFDD237 pKa = 3.99NDD239 pKa = 3.23SGLFTINVPVTPVIGFNDD257 pKa = 3.59IVRR260 pKa = 11.84LDD262 pKa = 4.21LVTQISPDD270 pKa = 3.58TVLDD274 pKa = 3.95TNNVPLAQYY283 pKa = 10.19RR284 pKa = 11.84YY285 pKa = 9.08GYY287 pKa = 10.25IYY289 pKa = 10.62GITPNDD295 pKa = 3.08VKK297 pKa = 11.41GIFNVNNDD305 pKa = 3.74SVYY308 pKa = 10.44MGLTDD313 pKa = 4.95GSPCIITAYY322 pKa = 10.48DD323 pKa = 3.73PVSGITNPAVSVTVDD338 pKa = 3.33GNITQSDD345 pKa = 4.61RR346 pKa = 11.84YY347 pKa = 10.2VISIPNDD354 pKa = 3.39YY355 pKa = 10.25AWEE358 pKa = 4.11VSSIDD363 pKa = 3.1GTAYY367 pKa = 9.76CVRR370 pKa = 11.84YY371 pKa = 10.19NRR373 pKa = 11.84TTGVFEE379 pKa = 6.02NIYY382 pKa = 10.21QYY384 pKa = 11.29QDD386 pKa = 3.03QTKK389 pKa = 9.05TNFIKK394 pKa = 10.63IPVGDD399 pKa = 3.77SQVRR403 pKa = 11.84FGGINFDD410 pKa = 4.1NPGSYY415 pKa = 10.9AKK417 pKa = 10.22IIVRR421 pKa = 11.84RR422 pKa = 11.84EE423 pKa = 3.62RR424 pKa = 11.84LSIGG428 pKa = 3.23

Molecular weight: 47.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B6D7J8|B6D7J8_9CAUD Gp25 OS=Listeria phage P40 OX=560178 PE=4 SV=1
MM1 pKa = 7.52VVILTFFIVGMCVGMFNVYY20 pKa = 10.18HH21 pKa = 5.63YY22 pKa = 10.03VAKK25 pKa = 9.95PALQEE30 pKa = 3.76LRR32 pKa = 11.84LLKK35 pKa = 10.78GEE37 pKa = 4.51PLPKK41 pKa = 9.47RR42 pKa = 11.84AKK44 pKa = 10.23FSIKK48 pKa = 8.74ITRR51 pKa = 3.45

Molecular weight: 5.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

11025

43

668

177.8

20.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.56 ± 0.353

0.744 ± 0.087

6.567 ± 0.316

7.138 ± 0.464

4.218 ± 0.248

6.113 ± 0.447

1.723 ± 0.177

7.356 ± 0.235

8.19 ± 0.471

8.444 ± 0.329

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.821 ± 0.27

5.905 ± 0.293

3.338 ± 0.213

3.42 ± 0.192

4.127 ± 0.274

5.651 ± 0.214

6.24 ± 0.329

6.948 ± 0.262

1.07 ± 0.15

4.426 ± 0.244
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski