Halomonas sp. JB380

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Halomonas; unclassified Halomonas

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3787 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1R4HZX8|A0A1R4HZX8_9GAMM Uncharacterized protein OS=Halomonas sp. JB380 OX=1434831 GN=CZ787_09525 PE=3 SV=1
MM1 pKa = 7.49AKK3 pKa = 9.96SSPFALIVVAVGGAVVGFLAAQTLSSDD30 pKa = 3.61ASSSSSVASQSAEE43 pKa = 3.8QTPTADD49 pKa = 3.57ALVGLDD55 pKa = 4.3SITLGEE61 pKa = 4.17RR62 pKa = 11.84LRR64 pKa = 11.84GEE66 pKa = 3.89ITASDD71 pKa = 4.4LLNGNDD77 pKa = 3.28GSRR80 pKa = 11.84YY81 pKa = 9.31DD82 pKa = 3.87RR83 pKa = 11.84YY84 pKa = 11.01MMTLDD89 pKa = 3.73EE90 pKa = 4.77SDD92 pKa = 4.93LIEE95 pKa = 4.41FSLRR99 pKa = 11.84GALNGIVALYY109 pKa = 10.48DD110 pKa = 3.74DD111 pKa = 4.4QLQLLSVAPTARR123 pKa = 11.84HH124 pKa = 6.21RR125 pKa = 11.84IEE127 pKa = 3.72QAGDD131 pKa = 3.35YY132 pKa = 9.16MVVVSGADD140 pKa = 3.16SSSYY144 pKa = 11.18GPYY147 pKa = 8.98TLNSRR152 pKa = 11.84VVEE155 pKa = 4.22LSDD158 pKa = 3.86STVLTLDD165 pKa = 3.91APLDD169 pKa = 3.5SWLDD173 pKa = 3.8GTSSEE178 pKa = 4.26YY179 pKa = 10.76TLTIEE184 pKa = 4.32EE185 pKa = 4.13AGMYY189 pKa = 8.38QLEE192 pKa = 4.23MRR194 pKa = 11.84SDD196 pKa = 3.37EE197 pKa = 3.9FDD199 pKa = 3.64AYY201 pKa = 11.37LEE203 pKa = 3.99LSGPNGYY210 pKa = 9.4FRR212 pKa = 11.84EE213 pKa = 4.71DD214 pKa = 3.79DD215 pKa = 3.99DD216 pKa = 5.1SAGNLDD222 pKa = 3.72ARR224 pKa = 11.84ISDD227 pKa = 3.73FLVPGDD233 pKa = 3.79YY234 pKa = 10.38TVTARR239 pKa = 11.84TSYY242 pKa = 11.52NEE244 pKa = 3.89GAGLFTLTAEE254 pKa = 4.29SRR256 pKa = 11.84EE257 pKa = 4.05LPGDD261 pKa = 3.64GEE263 pKa = 4.27LRR265 pKa = 11.84NDD267 pKa = 3.69GSIVPNDD274 pKa = 4.03SLNGLYY280 pKa = 10.33SGQNLSYY287 pKa = 10.3QVEE290 pKa = 4.08IEE292 pKa = 4.16EE293 pKa = 4.28AGMYY297 pKa = 9.55QIDD300 pKa = 4.38MMSDD304 pKa = 3.94DD305 pKa = 3.3IDD307 pKa = 4.9AYY309 pKa = 11.12LVLEE313 pKa = 5.06GPDD316 pKa = 3.27GYY318 pKa = 11.41YY319 pKa = 9.95RR320 pKa = 11.84EE321 pKa = 4.48NDD323 pKa = 3.59DD324 pKa = 5.81GGDD327 pKa = 3.62NLNARR332 pKa = 11.84IADD335 pKa = 3.78FFEE338 pKa = 5.12PGTYY342 pKa = 10.22QLTARR347 pKa = 11.84TAYY350 pKa = 10.31GSDD353 pKa = 2.91SGLFRR358 pKa = 11.84ITLEE362 pKa = 4.08PQEE365 pKa = 4.38LPEE368 pKa = 4.34GVEE371 pKa = 4.13LRR373 pKa = 11.84NSGAITLGEE382 pKa = 4.39TLNGWYY388 pKa = 8.94TGEE391 pKa = 3.99SLVYY395 pKa = 9.56EE396 pKa = 4.37LEE398 pKa = 4.16LAEE401 pKa = 4.63SSLVTINMRR410 pKa = 11.84SSDD413 pKa = 3.41FDD415 pKa = 4.08AYY417 pKa = 11.3LEE419 pKa = 4.25LEE421 pKa = 4.46GEE423 pKa = 4.7GVAVSDD429 pKa = 4.51DD430 pKa = 3.9DD431 pKa = 5.13GGAGTDD437 pKa = 3.6AQLQQQLLPGTYY449 pKa = 8.77TVIARR454 pKa = 11.84GFSTASSGLFEE465 pKa = 6.53LDD467 pKa = 3.16VTAEE471 pKa = 4.07PAQMQPNMM479 pKa = 4.11

Molecular weight: 51.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1R4HUY1|A0A1R4HUY1_9GAMM ATP synthase subunit delta OS=Halomonas sp. JB380 OX=1434831 GN=atpH PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.21NGRR28 pKa = 11.84AVISRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.97GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3787

0

3787

1169929

37

2452

308.9

33.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.754 ± 0.041

0.924 ± 0.014

5.381 ± 0.037

6.176 ± 0.039

3.61 ± 0.027

7.708 ± 0.04

2.422 ± 0.021

5.284 ± 0.032

3.198 ± 0.036

11.248 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.651 ± 0.019

3.152 ± 0.025

4.707 ± 0.025

4.346 ± 0.037

6.142 ± 0.037

5.911 ± 0.027

5.287 ± 0.025

7.151 ± 0.034

1.424 ± 0.018

2.524 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski