Pseudomonas fluorescens (strain SBW25)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; Pseudomonas fluorescens group; Pseudomonas fluorescens

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6388 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A4V771|A4V771_PSEFS RNA polymerase sigma factor OS=Pseudomonas fluorescens (strain SBW25) OX=216595 GN=pQBR0350 PE=3 SV=1
MM1 pKa = 7.45NNPTSTATFAPNKK14 pKa = 8.92STAEE18 pKa = 4.18VYY20 pKa = 10.35TNTGSLPYY28 pKa = 10.09DD29 pKa = 3.54YY30 pKa = 10.95EE31 pKa = 4.64DD32 pKa = 4.33FGLSRR37 pKa = 11.84SEE39 pKa = 4.03LVAKK43 pKa = 10.17FLEE46 pKa = 4.89AGEE49 pKa = 4.23HH50 pKa = 5.88EE51 pKa = 4.87YY52 pKa = 11.26YY53 pKa = 10.78LRR55 pKa = 11.84SDD57 pKa = 3.37WSAANPVGEE66 pKa = 4.34QTVPDD71 pKa = 3.42YY72 pKa = 10.43WDD74 pKa = 2.75WVMAQIAQDD83 pKa = 3.9DD84 pKa = 3.8EE85 pKa = 6.45DD86 pKa = 4.21YY87 pKa = 11.76

Molecular weight: 9.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C3K1J0|C3K1J0_PSEFS Putative proteic killer suppression protein OS=Pseudomonas fluorescens (strain SBW25) OX=216595 GN=PFLU_5031 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIKK11 pKa = 10.52RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.38GRR39 pKa = 11.84ARR41 pKa = 11.84LAVV44 pKa = 3.42

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6388

0

6388

2089672

24

4690

327.1

35.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.91 ± 0.036

0.971 ± 0.011

5.349 ± 0.022

5.479 ± 0.03

3.684 ± 0.019

7.853 ± 0.034

2.302 ± 0.019

4.878 ± 0.023

3.636 ± 0.031

11.625 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.335 ± 0.016

3.144 ± 0.026

4.875 ± 0.025

4.534 ± 0.029

6.137 ± 0.028

5.959 ± 0.025

5.128 ± 0.022

7.216 ± 0.024

1.439 ± 0.015

2.546 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski