bacterium HR15

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2671 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5WS41|A0A2H5WS41_9BACT Site-specific DNA-methyltransferase (adenine-specific) OS=bacterium HR15 OX=2035410 GN=dpnM_1 PE=3 SV=1
MM1 pKa = 7.09MMNRR5 pKa = 11.84LIALPIALAFVCGLVLAQPRR25 pKa = 11.84IVINEE30 pKa = 4.1FAYY33 pKa = 10.7DD34 pKa = 3.72DD35 pKa = 3.92TSTDD39 pKa = 3.13DD40 pKa = 3.54VEE42 pKa = 5.47FVEE45 pKa = 6.06LYY47 pKa = 10.77NADD50 pKa = 4.03TVPVDD55 pKa = 3.08ISGWRR60 pKa = 11.84LEE62 pKa = 4.29SGDD65 pKa = 4.82LVSGDD70 pKa = 4.04NNPDD74 pKa = 3.24YY75 pKa = 10.02TIPGAPGSGTVVLQPGQFYY94 pKa = 10.98VIGSEE99 pKa = 4.21LVPNVNLVVGANNLFEE115 pKa = 5.86NDD117 pKa = 4.01NEE119 pKa = 4.08WTALKK124 pKa = 9.76MPDD127 pKa = 3.73GMVVDD132 pKa = 5.84AIAYY136 pKa = 5.35EE137 pKa = 4.49TNKK140 pKa = 10.52APDD143 pKa = 3.34LTTWVPANIIPQLGRR158 pKa = 11.84GIWGNYY164 pKa = 5.24ITLQASTTTDD174 pKa = 3.35DD175 pKa = 4.0TLLSLQRR182 pKa = 11.84WRR184 pKa = 11.84DD185 pKa = 3.59GLDD188 pKa = 3.21TNDD191 pKa = 3.24NGRR194 pKa = 11.84DD195 pKa = 3.8FGHH198 pKa = 6.33MPWTPGASNNTTPTPFSQNFNALNVYY224 pKa = 10.51DD225 pKa = 4.93LVPNLSGSFRR235 pKa = 11.84APRR238 pKa = 11.84VIDD241 pKa = 3.41PTQCDD246 pKa = 3.21TTYY249 pKa = 10.66ATTPNPNCIPASPDD263 pKa = 2.83GGNAMIGWDD272 pKa = 3.27WAGGGNMATSTFAFQNRR289 pKa = 11.84AGYY292 pKa = 10.34DD293 pKa = 3.32LLIYY297 pKa = 9.79IDD299 pKa = 4.15NSMPIPGEE307 pKa = 3.98LEE309 pKa = 3.52SWMIGLMGTCDD320 pKa = 3.35SFYY323 pKa = 10.67NIPFRR328 pKa = 11.84CLVNASGPTGVGWMFKK344 pKa = 10.22RR345 pKa = 11.84ANTANDD351 pKa = 3.23PMEE354 pKa = 4.43VKK356 pKa = 10.51LRR358 pKa = 11.84LVDD361 pKa = 4.33AGPGGDD367 pKa = 4.23SNPGCGTAGTMQWTTFGEE385 pKa = 4.23IDD387 pKa = 3.94LSNYY391 pKa = 7.85TPGWYY396 pKa = 9.9RR397 pKa = 11.84LRR399 pKa = 11.84LEE401 pKa = 4.22VKK403 pKa = 8.95GDD405 pKa = 3.53KK406 pKa = 10.8VVGTFSNPDD415 pKa = 2.82GSGAITFSGTTEE427 pKa = 3.94GCIGAFYY434 pKa = 10.24IGYY437 pKa = 9.35RR438 pKa = 11.84EE439 pKa = 4.93AYY441 pKa = 6.01TTDD444 pKa = 3.24PFANPVRR451 pKa = 11.84IDD453 pKa = 3.53ALSLFIPTDD462 pKa = 3.38GDD464 pKa = 3.45VDD466 pKa = 4.26GNGCVDD472 pKa = 4.28DD473 pKa = 5.59ADD475 pKa = 4.1LLEE478 pKa = 4.02VLFAFGATGCTRR490 pKa = 11.84ADD492 pKa = 3.47VNGDD496 pKa = 3.72GVVDD500 pKa = 4.42DD501 pKa = 5.53ADD503 pKa = 4.19LLTVLFDD510 pKa = 4.6FGSGCC515 pKa = 4.3

Molecular weight: 55.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5WXW9|A0A2H5WXW9_9BACT Uncharacterized protein OS=bacterium HR15 OX=2035410 GN=HRbin15_02295 PE=4 SV=1
MM1 pKa = 6.96FRR3 pKa = 11.84KK4 pKa = 10.09VLGKK8 pKa = 10.06RR9 pKa = 11.84LKK11 pKa = 9.94TLTTRR16 pKa = 11.84FNSALQVVKK25 pKa = 10.56IRR27 pKa = 11.84LLSVVV32 pKa = 3.49

Molecular weight: 3.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2671

0

2671

916987

29

2176

343.3

38.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.481 ± 0.051

1.06 ± 0.022

4.532 ± 0.038

6.605 ± 0.053

3.541 ± 0.029

7.716 ± 0.054

2.292 ± 0.027

5.201 ± 0.033

2.704 ± 0.031

11.207 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.223 ± 0.022

2.824 ± 0.037

5.818 ± 0.036

4.625 ± 0.044

7.827 ± 0.043

4.835 ± 0.041

5.218 ± 0.038

7.229 ± 0.04

1.88 ± 0.027

3.182 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski