Rhodobacteraceae bacterium SB2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; unclassified Rhodobacteraceae

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3489 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A177H6R6|A0A177H6R6_9RHOB Bacterial regulatory proteinC Fis family OS=Rhodobacteraceae bacterium SB2 OX=1689867 GN=pfor_25c2641 PE=4 SV=1
MM1 pKa = 7.77AIKK4 pKa = 10.37SVTVFIDD11 pKa = 3.24QFSDD15 pKa = 3.68RR16 pKa = 11.84IFDD19 pKa = 4.17LGHH22 pKa = 6.82DD23 pKa = 4.02NIYY26 pKa = 10.7LYY28 pKa = 10.49DD29 pKa = 3.67YY30 pKa = 11.33GNFHH34 pKa = 5.93EE35 pKa = 5.29TVWTDD40 pKa = 2.5NYY42 pKa = 10.85YY43 pKa = 10.93NVSTGAFSGYY53 pKa = 9.9FSYY56 pKa = 11.23DD57 pKa = 2.75DD58 pKa = 4.41SYY60 pKa = 11.7FSRR63 pKa = 11.84TDD65 pKa = 3.42GVGGFYY71 pKa = 10.56LVSPDD76 pKa = 3.65SDD78 pKa = 3.62VSLVDD83 pKa = 3.36PTFNAATTNNWDD95 pKa = 3.36YY96 pKa = 11.62DD97 pKa = 3.79LRR99 pKa = 11.84ASDD102 pKa = 3.94SGSLVDD108 pKa = 3.6SLGYY112 pKa = 9.76IAFYY116 pKa = 9.33DD117 pKa = 3.73TYY119 pKa = 10.73DD120 pKa = 3.3TLFRR124 pKa = 11.84TDD126 pKa = 3.17QNDD129 pKa = 3.76DD130 pKa = 3.16VSPNHH135 pKa = 7.01GDD137 pKa = 3.04WTLAAYY143 pKa = 10.38LDD145 pKa = 4.1EE146 pKa = 6.05LEE148 pKa = 5.72NPDD151 pKa = 3.75QNEE154 pKa = 4.3IICIDD159 pKa = 3.44VDD161 pKa = 4.06TLNGASSHH169 pKa = 5.02YY170 pKa = 10.78SKK172 pKa = 10.87LFKK175 pKa = 10.13STLSVEE181 pKa = 3.71RR182 pKa = 11.84WLAGDD187 pKa = 4.1LVYY190 pKa = 10.69ISGLEE195 pKa = 4.08AILEE199 pKa = 4.24NFFTLNDD206 pKa = 3.28KK207 pKa = 10.81RR208 pKa = 11.84LTGNISDD215 pKa = 4.94DD216 pKa = 3.97EE217 pKa = 4.51FTVSCLSVSIAGAPATQEE235 pKa = 4.11LSTLNLLSSLSIPIIQAAPNVGQGNYY261 pKa = 10.19DD262 pKa = 3.07WGSNYY267 pKa = 10.32ADD269 pKa = 4.27VINVGAWNIDD279 pKa = 3.57NEE281 pKa = 4.52DD282 pKa = 3.87EE283 pKa = 4.62LLLSSISTLSTLDD296 pKa = 3.13ILGNGQVLKK305 pKa = 10.65SDD307 pKa = 3.39WGTGSNFGTSFATPKK322 pKa = 10.08VAADD326 pKa = 3.07ITNFYY331 pKa = 10.95SSFISAIEE339 pKa = 4.08SEE341 pKa = 4.48GTSLGEE347 pKa = 4.38AQAQSQALNIDD358 pKa = 3.4YY359 pKa = 10.95SDD361 pKa = 3.7VVNVLLEE368 pKa = 4.17QISTEE373 pKa = 4.44LIVTLDD379 pKa = 3.61LAGNQATYY387 pKa = 9.74HH388 pKa = 5.77PKK390 pKa = 10.11VRR392 pKa = 11.84TSDD395 pKa = 3.67VNVSPTPVKK404 pKa = 9.61ITSTSGGLSGLTVASARR421 pKa = 11.84TNDD424 pKa = 3.27APIFTSGAEE433 pKa = 4.21SVALEE438 pKa = 4.54NITLSSVIYY447 pKa = 10.41NATAEE452 pKa = 4.63DD453 pKa = 3.81SDD455 pKa = 4.52GDD457 pKa = 3.65QLTYY461 pKa = 11.43SLVNSLNILVSGEE474 pKa = 4.24SAVGEE479 pKa = 3.79IDD481 pKa = 3.13IALFDD486 pKa = 5.97DD487 pKa = 4.57ISPLHH492 pKa = 6.59AEE494 pKa = 4.19RR495 pKa = 11.84LGTLAAEE502 pKa = 4.67GAYY505 pKa = 10.45NDD507 pKa = 3.92VVFHH511 pKa = 6.1RR512 pKa = 11.84VIDD515 pKa = 3.87GFMAQTGDD523 pKa = 3.37VQYY526 pKa = 11.26GKK528 pKa = 10.54RR529 pKa = 11.84NNSIEE534 pKa = 3.87NAGRR538 pKa = 11.84GGSNKK543 pKa = 9.02PDD545 pKa = 3.23LVAEE549 pKa = 4.82FSTIPFDD556 pKa = 3.96RR557 pKa = 11.84GIVGMARR564 pKa = 11.84SSDD567 pKa = 3.72PNSANSQFFMMFEE580 pKa = 4.19DD581 pKa = 4.93TYY583 pKa = 11.55SLNGDD588 pKa = 3.66YY589 pKa = 10.77TVVGVVASGLDD600 pKa = 3.37VLDD603 pKa = 4.72LIKK606 pKa = 10.95RR607 pKa = 11.84GDD609 pKa = 3.37ASQNGVVSQNPDD621 pKa = 2.86YY622 pKa = 10.41MEE624 pKa = 4.26EE625 pKa = 4.04VTYY628 pKa = 10.4TPATDD633 pKa = 2.95IFEE636 pKa = 4.7INSTSGEE643 pKa = 3.74ITFINEE649 pKa = 3.86PNFSNMDD656 pKa = 3.56FKK658 pKa = 11.64DD659 pKa = 3.55LVVAVSDD666 pKa = 4.18GSEE669 pKa = 4.01TVYY672 pKa = 9.46KK673 pKa = 10.19TVTLDD678 pKa = 4.7LPPQNITISFSDD690 pKa = 3.4INSVEE695 pKa = 4.13SQVPDD700 pKa = 3.16NDD702 pKa = 4.1LVFKK706 pKa = 10.71GGYY709 pKa = 8.68DD710 pKa = 3.09ATVAVEE716 pKa = 3.85NGKK719 pKa = 10.36LGVLSGEE726 pKa = 3.8ISFTHH731 pKa = 6.57VEE733 pKa = 4.32FASQSYY739 pKa = 10.51DD740 pKa = 3.06HH741 pKa = 7.34GIAISDD747 pKa = 3.73VVLQLRR753 pKa = 11.84DD754 pKa = 3.48IVGLSTLSGNQKK766 pKa = 7.94TAADD770 pKa = 3.7IDD772 pKa = 4.0GDD774 pKa = 4.09GDD776 pKa = 3.91VAISDD781 pKa = 3.82VVSNLRR787 pKa = 11.84HH788 pKa = 5.4IVGLDD793 pKa = 3.48TIEE796 pKa = 3.83QCALVDD802 pKa = 3.61TSDD805 pKa = 3.54QLVTGLTSSTIADD818 pKa = 3.65LTLIQLGDD826 pKa = 3.48VDD828 pKa = 5.48LSATFEE834 pKa = 4.11IAA836 pKa = 3.82

Molecular weight: 89.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A177HCU2|A0A177HCU2_9RHOB Phytoene desaturase (Neurosporene-forming) OS=Rhodobacteraceae bacterium SB2 OX=1689867 GN=crtI PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.43EE41 pKa = 3.72LSAA44 pKa = 5.03

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3489

0

3489

1066040

29

4255

305.5

33.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.136 ± 0.054

1.049 ± 0.014

5.608 ± 0.047

5.65 ± 0.036

4.17 ± 0.03

8.006 ± 0.05

2.12 ± 0.022

6.032 ± 0.033

4.177 ± 0.031

10.31 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.765 ± 0.022

3.313 ± 0.029

4.578 ± 0.029

3.921 ± 0.026

5.494 ± 0.036

6.104 ± 0.042

5.159 ± 0.037

6.61 ± 0.033

1.338 ± 0.017

2.46 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski