Tomelloso virus

Taxonomy: Viruses; Naldaviricetes; Lefavirales; Nudiviridae; Alphanudivirus; Drosophila melanogaster nudivirus B

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4T2W2|A0A2H4T2W2_9VIRU OrNV gp035-like protein OS=Tomelloso virus OX=2053981 PE=4 SV=1
MM1 pKa = 7.56DD2 pKa = 4.78QIHH5 pKa = 6.93DD6 pKa = 4.09AFVEE10 pKa = 4.05LLTLGIITDD19 pKa = 3.66SDD21 pKa = 4.01EE22 pKa = 4.46EE23 pKa = 4.3NVTIDD28 pKa = 5.01LEE30 pKa = 4.29ALYY33 pKa = 10.89SLGYY37 pKa = 9.58VLVYY41 pKa = 10.96NLTIPVFTTILNYY54 pKa = 10.77NYY56 pKa = 9.28MYY58 pKa = 10.92DD59 pKa = 4.26GILYY63 pKa = 8.34TSNVVPRR70 pKa = 11.84RR71 pKa = 11.84ALSTFVLSKK80 pKa = 9.99IQTIGVSDD88 pKa = 4.88FKK90 pKa = 11.59

Molecular weight: 10.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4T2T7|A0A2H4T2T7_9VIRU GrBNV gp61-like protein OS=Tomelloso virus OX=2053981 PE=4 SV=1
MM1 pKa = 7.24NVYY4 pKa = 8.28ITNISEE10 pKa = 4.98FINSIHH16 pKa = 7.71DD17 pKa = 4.36YY18 pKa = 8.33ITEE21 pKa = 4.06RR22 pKa = 11.84YY23 pKa = 9.17EE24 pKa = 3.55IVPRR28 pKa = 11.84DD29 pKa = 3.95FVTKK33 pKa = 10.56LIVGNNALAWHH44 pKa = 6.69SEE46 pKa = 4.14TQQMEE51 pKa = 4.26EE52 pKa = 3.78FHH54 pKa = 6.09CQKK57 pKa = 10.3FKK59 pKa = 11.11KK60 pKa = 9.63HH61 pKa = 5.02LHH63 pKa = 3.98IHH65 pKa = 6.1KK66 pKa = 9.69NYY68 pKa = 10.25KK69 pKa = 10.11NIVYY73 pKa = 9.92CCTEE77 pKa = 3.43VDD79 pKa = 4.41IIALKK84 pKa = 10.2YY85 pKa = 10.51LNASIFGNLKK95 pKa = 10.46NEE97 pKa = 4.78IISSLHH103 pKa = 5.13SWLPAATFQHH113 pKa = 5.19VRR115 pKa = 11.84VTIKK119 pKa = 10.43PATSKK124 pKa = 10.12IDD126 pKa = 3.48SFFEE130 pKa = 3.97PSYY133 pKa = 11.19LFYY136 pKa = 10.49LQSYY140 pKa = 8.66IDD142 pKa = 4.38HH143 pKa = 7.62PIDD146 pKa = 2.85ICYY149 pKa = 9.89TYY151 pKa = 10.22FHH153 pKa = 7.14SNIVFYY159 pKa = 10.9KK160 pKa = 10.01SYY162 pKa = 8.92TLKK165 pKa = 10.78GYY167 pKa = 9.34RR168 pKa = 11.84EE169 pKa = 4.01KK170 pKa = 11.18LIMVRR175 pKa = 11.84ARR177 pKa = 11.84SPSPARR183 pKa = 11.84RR184 pKa = 11.84RR185 pKa = 11.84RR186 pKa = 11.84RR187 pKa = 11.84SRR189 pKa = 11.84SPSRR193 pKa = 11.84RR194 pKa = 11.84RR195 pKa = 11.84SPARR199 pKa = 11.84RR200 pKa = 11.84SRR202 pKa = 11.84SSVKK206 pKa = 10.04KK207 pKa = 9.06RR208 pKa = 11.84AGRR211 pKa = 11.84PRR213 pKa = 11.84SRR215 pKa = 11.84SRR217 pKa = 11.84SRR219 pKa = 11.84SRR221 pKa = 11.84SRR223 pKa = 11.84SRR225 pKa = 11.84SS226 pKa = 3.31

Molecular weight: 26.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

93

0

93

31393

78

1252

337.6

38.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.326 ± 0.165

2.16 ± 0.115

6.342 ± 0.184

5.562 ± 0.183

4.405 ± 0.164

3.692 ± 0.116

1.899 ± 0.11

8.04 ± 0.134

6.619 ± 0.193

9.002 ± 0.165

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.631 ± 0.101

6.699 ± 0.159

4.237 ± 0.152

3.504 ± 0.158

4.154 ± 0.169

8.113 ± 0.345

6.384 ± 0.19

5.68 ± 0.158

0.714 ± 0.053

4.839 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski