Pseudomonas phage JBD25

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J9SNL4|J9SNL4_9CAUD Uncharacterized protein OS=Pseudomonas phage JBD25 OX=1225792 GN=HMPREFV_HMPID9847gp0055 PE=4 SV=1
MM1 pKa = 7.46SFNSRR6 pKa = 11.84EE7 pKa = 3.92SSLVDD12 pKa = 3.44GQPVRR17 pKa = 11.84LYY19 pKa = 10.17QFSRR23 pKa = 11.84GAIRR27 pKa = 11.84WSYY30 pKa = 10.82NSSDD34 pKa = 4.7RR35 pKa = 11.84DD36 pKa = 3.1ITYY39 pKa = 10.2QNQVFRR45 pKa = 11.84TVPGGITDD53 pKa = 3.54NGIICSGDD61 pKa = 3.43PQSDD65 pKa = 3.53QFVITAPADD74 pKa = 3.6LDD76 pKa = 3.68VALLYY81 pKa = 8.52KK82 pKa = 10.05TRR84 pKa = 11.84SPSGAIDD91 pKa = 3.52LVVYY95 pKa = 10.58DD96 pKa = 3.99MHH98 pKa = 8.19YY99 pKa = 11.19GDD101 pKa = 5.63AEE103 pKa = 4.2AAVSWVGQIGDD114 pKa = 4.0VDD116 pKa = 3.86WPTVDD121 pKa = 3.06SCRR124 pKa = 11.84ITCVSEE130 pKa = 4.47DD131 pKa = 3.9EE132 pKa = 6.08LMDD135 pKa = 3.76QPGLIDD141 pKa = 3.86TYY143 pKa = 11.23CRR145 pKa = 11.84TCTAVVGDD153 pKa = 4.48HH154 pKa = 6.1RR155 pKa = 11.84CKK157 pKa = 11.04VNLVPYY163 pKa = 9.97RR164 pKa = 11.84VTLTPQSISGWVISSGVVAGYY185 pKa = 11.08ADD187 pKa = 3.46GWFTGGYY194 pKa = 8.66VEE196 pKa = 4.37WQVDD200 pKa = 3.02GDD202 pKa = 4.29NYY204 pKa = 10.6DD205 pKa = 2.73SRR207 pKa = 11.84YY208 pKa = 9.2IEE210 pKa = 4.13RR211 pKa = 11.84HH212 pKa = 5.85AGPDD216 pKa = 3.48LYY218 pKa = 10.62ILGGTEE224 pKa = 5.08GIPAGGQLRR233 pKa = 11.84VYY235 pKa = 9.42PGCDD239 pKa = 3.1GLAQTCDD246 pKa = 3.63DD247 pKa = 4.57KK248 pKa = 11.59FSNLPNFRR256 pKa = 11.84GFNAMQGKK264 pKa = 9.57SPFDD268 pKa = 4.06GDD270 pKa = 4.13QVWW273 pKa = 3.01

Molecular weight: 29.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J9STP5|J9STP5_9CAUD Integrase core domain protein OS=Pseudomonas phage JBD25 OX=1225792 GN=HMPREFV_HMPID9847gp0014 PE=4 SV=1
MM1 pKa = 7.43SVEE4 pKa = 3.86AYY6 pKa = 9.26IRR8 pKa = 11.84GMAARR13 pKa = 11.84GFSRR17 pKa = 11.84SAAAAALGMHH27 pKa = 6.19WVKK30 pKa = 10.86FMDD33 pKa = 4.19LLEE36 pKa = 4.46RR37 pKa = 11.84MPDD40 pKa = 3.48IEE42 pKa = 3.84WGYY45 pKa = 9.72PYY47 pKa = 10.86KK48 pKa = 10.97SFDD51 pKa = 3.46RR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84HH55 pKa = 4.75AKK57 pKa = 8.96NLKK60 pKa = 9.27GYY62 pKa = 9.66RR63 pKa = 11.84FRR65 pKa = 11.84DD66 pKa = 3.25SEE68 pKa = 3.95GRR70 pKa = 11.84RR71 pKa = 11.84RR72 pKa = 11.84SVAALRR78 pKa = 11.84AVNQARR84 pKa = 11.84RR85 pKa = 11.84HH86 pKa = 5.7EE87 pKa = 4.21YY88 pKa = 9.63TVFGVTDD95 pKa = 3.54SLSNLVKK102 pKa = 10.76RR103 pKa = 11.84FGCVAKK109 pKa = 9.97STVQKK114 pKa = 10.6RR115 pKa = 11.84LAKK118 pKa = 10.33GMSIEE123 pKa = 4.25QALTTPRR130 pKa = 11.84SDD132 pKa = 3.27HH133 pKa = 7.13LSGLKK138 pKa = 9.9RR139 pKa = 11.84KK140 pKa = 9.64PEE142 pKa = 3.72SHH144 pKa = 5.74PWKK147 pKa = 8.72RR148 pKa = 11.84AEE150 pKa = 3.75RR151 pKa = 11.84RR152 pKa = 11.84GVINHH157 pKa = 6.65RR158 pKa = 11.84EE159 pKa = 3.9RR160 pKa = 11.84QLKK163 pKa = 9.93AKK165 pKa = 9.99RR166 pKa = 11.84DD167 pKa = 3.47QRR169 pKa = 11.84QAEE172 pKa = 4.21EE173 pKa = 4.13RR174 pKa = 11.84LHH176 pKa = 6.24GG177 pKa = 4.22

Molecular weight: 20.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

12574

52

1209

228.6

25.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.176 ± 0.703

0.915 ± 0.15

5.885 ± 0.227

6.259 ± 0.372

2.879 ± 0.177

7.579 ± 0.371

1.805 ± 0.209

4.143 ± 0.203

4.016 ± 0.321

10.021 ± 0.298

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.195 ± 0.142

3.046 ± 0.18

4.819 ± 0.328

5.193 ± 0.307

7.277 ± 0.312

5.694 ± 0.226

5.36 ± 0.323

6.418 ± 0.294

1.734 ± 0.16

2.585 ± 0.21
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski