Streptomyces phage Gilson

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gilsonvirus; Streptomyces virus Gilson

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 229 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3T0ICQ4|A0A3T0ICQ4_9CAUD Uncharacterized protein OS=Streptomyces phage Gilson OX=2488789 GN=87 PE=4 SV=1
MM1 pKa = 7.58LNLSDD6 pKa = 6.04LEE8 pKa = 4.35NQGLTVKK15 pKa = 10.16KK16 pKa = 10.48FCGLDD21 pKa = 2.95IQEE24 pKa = 4.61NVGCTGEE31 pKa = 4.08AVGDD35 pKa = 3.88IVAEE39 pKa = 4.33MEE41 pKa = 5.15DD42 pKa = 3.97GMQIPIPVCQQHH54 pKa = 6.78LDD56 pKa = 3.9VIQSHH61 pKa = 6.87FDD63 pKa = 3.52VEE65 pKa = 4.72SLGG68 pKa = 3.61

Molecular weight: 7.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q9R4T0|A0A3Q9R4T0_9CAUD HNH endonuclease OS=Streptomyces phage Gilson OX=2488789 GN=94 PE=4 SV=1
MM1 pKa = 7.47FEE3 pKa = 3.96FSEE6 pKa = 4.76EE7 pKa = 3.9YY8 pKa = 10.03KK9 pKa = 10.57RR10 pKa = 11.84YY11 pKa = 9.77IGSDD15 pKa = 2.68RR16 pKa = 11.84WKK18 pKa = 10.43LVCARR23 pKa = 11.84YY24 pKa = 6.78WTTFGRR30 pKa = 11.84KK31 pKa = 8.47CQACGSRR38 pKa = 11.84KK39 pKa = 9.31NLHH42 pKa = 5.16VHH44 pKa = 6.02HH45 pKa = 6.37NTYY48 pKa = 10.6EE49 pKa = 4.06RR50 pKa = 11.84FGRR53 pKa = 11.84EE54 pKa = 3.62RR55 pKa = 11.84LSDD58 pKa = 3.59LTGVCQSCHH67 pKa = 5.15KK68 pKa = 9.99VIHH71 pKa = 6.67AIHH74 pKa = 6.27RR75 pKa = 11.84RR76 pKa = 11.84DD77 pKa = 3.17RR78 pKa = 11.84RR79 pKa = 11.84KK80 pKa = 10.05SLRR83 pKa = 11.84SVTLSFVNKK92 pKa = 9.59KK93 pKa = 10.05RR94 pKa = 11.84LSRR97 pKa = 11.84LL98 pKa = 3.19

Molecular weight: 11.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

229

0

229

36068

29

2100

157.5

17.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.035 ± 0.416

1.056 ± 0.124

6.416 ± 0.197

7.009 ± 0.294

3.945 ± 0.122

7.794 ± 0.202

1.891 ± 0.137

5.337 ± 0.158

6.288 ± 0.289

6.998 ± 0.166

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.85 ± 0.123

4.741 ± 0.163

3.665 ± 0.143

3.15 ± 0.205

5.617 ± 0.209

5.933 ± 0.256

5.922 ± 0.34

7.253 ± 0.193

1.963 ± 0.106

4.137 ± 0.177
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski