Tomato associated geminivirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; unclassified Geminiviridae

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A223LU27|A0A223LU27_9GEMI Uncharacterized protein OS=Tomato associated geminivirus 1 OX=2027645 PE=4 SV=1
MM1 pKa = 8.36DD2 pKa = 4.19IPLYY6 pKa = 10.19EE7 pKa = 4.28VNDD10 pKa = 3.63YY11 pKa = 11.07PGVYY15 pKa = 10.02NQFLQTCANRR25 pKa = 11.84LISWQLSKK33 pKa = 11.29VQLEE37 pKa = 4.33LDD39 pKa = 3.77VKK41 pKa = 10.9GISIDD46 pKa = 3.91RR47 pKa = 11.84KK48 pKa = 9.0EE49 pKa = 4.12QLLKK53 pKa = 10.98LQQ55 pKa = 4.02

Molecular weight: 6.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A223LU26|A0A223LU26_9GEMI Replication-associated protein OS=Tomato associated geminivirus 1 OX=2027645 PE=3 SV=1
MM1 pKa = 7.8AEE3 pKa = 4.57RR4 pKa = 11.84KK5 pKa = 9.63RR6 pKa = 11.84KK7 pKa = 9.13MEE9 pKa = 3.6WSKK12 pKa = 11.78ANRR15 pKa = 11.84KK16 pKa = 9.07RR17 pKa = 11.84SRR19 pKa = 11.84TTLPRR24 pKa = 11.84NSRR27 pKa = 11.84VYY29 pKa = 10.38ARR31 pKa = 11.84KK32 pKa = 9.65RR33 pKa = 11.84PILRR37 pKa = 11.84GPKK40 pKa = 8.52LTSRR44 pKa = 11.84DD45 pKa = 3.19KK46 pKa = 10.68VYY48 pKa = 10.43TYY50 pKa = 10.13TKK52 pKa = 10.91YY53 pKa = 8.87EE54 pKa = 4.09TLTTAGAVYY63 pKa = 10.11HH64 pKa = 6.49LNSFSQGLANNQRR77 pKa = 11.84ATSVDD82 pKa = 2.79IVHH85 pKa = 6.43NVQLRR90 pKa = 11.84FSVEE94 pKa = 4.03LPTGAMVFCRR104 pKa = 11.84RR105 pKa = 11.84YY106 pKa = 8.56KK107 pKa = 9.93MSWAVVQDD115 pKa = 3.63MMPGDD120 pKa = 4.23SMPAVSDD127 pKa = 3.54IFSMTSPPIEE137 pKa = 4.14GLEE140 pKa = 4.01YY141 pKa = 10.4IADD144 pKa = 4.02DD145 pKa = 3.23NHH147 pKa = 7.1RR148 pKa = 11.84RR149 pKa = 11.84FQVKK153 pKa = 9.89KK154 pKa = 10.43FGILYY159 pKa = 9.9LEE161 pKa = 4.71SGGADD166 pKa = 3.12YY167 pKa = 11.26SVNTGKK173 pKa = 8.21QTYY176 pKa = 9.23NVQDD180 pKa = 3.25ALKK183 pKa = 10.28RR184 pKa = 11.84VFVKK188 pKa = 10.74CNTRR192 pKa = 11.84GVYY195 pKa = 10.13DD196 pKa = 4.0SNSSAGSISAMNSGALYY213 pKa = 9.88FVVNPTFAGYY223 pKa = 10.41LGFNITVYY231 pKa = 10.69HH232 pKa = 6.31NSPVV236 pKa = 3.07

Molecular weight: 26.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

971

55

332

194.2

22.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.252 ± 0.556

1.751 ± 0.36

5.252 ± 0.849

5.149 ± 0.677

4.943 ± 0.4

4.737 ± 0.657

1.648 ± 0.39

6.694 ± 0.891

5.149 ± 0.968

6.385 ± 0.839

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.648 ± 0.571

6.076 ± 0.155

6.076 ± 0.948

5.561 ± 0.907

6.9 ± 0.938

7.518 ± 0.959

6.076 ± 0.518

5.458 ± 1.733

1.854 ± 0.373

5.87 ± 0.458
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski