Neisseria wadsworthii 9715

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae; Neisseria; Neisseria wadsworthii

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2553 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G4CPU6|G4CPU6_9NEIS Uncharacterized protein OS=Neisseria wadsworthii 9715 OX=1030841 GN=HMPREF9370_1106 PE=4 SV=1
MM1 pKa = 7.67TYY3 pKa = 10.32GIKK6 pKa = 9.96VGKK9 pKa = 10.29YY10 pKa = 9.0NDD12 pKa = 4.73GYY14 pKa = 10.93SQAINNSAWYY24 pKa = 9.97YY25 pKa = 9.83DD26 pKa = 3.46EE27 pKa = 5.81KK28 pKa = 11.39YY29 pKa = 10.5SGKK32 pKa = 10.77GSDD35 pKa = 3.55KK36 pKa = 11.02KK37 pKa = 10.29HH38 pKa = 5.63GKK40 pKa = 9.97KK41 pKa = 9.51KK42 pKa = 10.89DD43 pKa = 3.43KK44 pKa = 11.01DD45 pKa = 3.48WDD47 pKa = 3.46DD48 pKa = 4.46DD49 pKa = 4.71YY50 pKa = 11.93YY51 pKa = 11.63DD52 pKa = 4.83KK53 pKa = 11.18KK54 pKa = 11.42GKK56 pKa = 10.4DD57 pKa = 3.42KK58 pKa = 11.29DD59 pKa = 3.32WDD61 pKa = 3.65DD62 pKa = 5.0KK63 pKa = 11.52YY64 pKa = 11.75DD65 pKa = 3.91KK66 pKa = 10.67KK67 pKa = 11.46GKK69 pKa = 10.39DD70 pKa = 3.47KK71 pKa = 11.25DD72 pKa = 3.44WDD74 pKa = 4.23DD75 pKa = 4.46DD76 pKa = 4.61CDD78 pKa = 4.88RR79 pKa = 11.84CDD81 pKa = 4.46DD82 pKa = 4.68DD83 pKa = 5.83YY84 pKa = 12.2NVITGDD90 pKa = 3.36DD91 pKa = 4.27CDD93 pKa = 4.16NVLCGTEE100 pKa = 4.38CNDD103 pKa = 5.37KK104 pKa = 10.72IDD106 pKa = 4.42GKK108 pKa = 10.64DD109 pKa = 3.67GNDD112 pKa = 3.44TIYY115 pKa = 11.03GKK117 pKa = 11.03GGDD120 pKa = 3.9DD121 pKa = 4.19LLYY124 pKa = 11.15GGDD127 pKa = 3.59GCDD130 pKa = 3.94YY131 pKa = 10.71IDD133 pKa = 4.88GGEE136 pKa = 4.35GCDD139 pKa = 4.16TICGGDD145 pKa = 3.8GDD147 pKa = 5.74DD148 pKa = 4.21ILIGGGGNDD157 pKa = 3.51TLYY160 pKa = 11.31GGDD163 pKa = 4.59GNDD166 pKa = 3.54KK167 pKa = 10.98LYY169 pKa = 11.18GSNEE173 pKa = 3.66ADD175 pKa = 3.29CLYY178 pKa = 11.33GEE180 pKa = 5.34DD181 pKa = 4.98GCDD184 pKa = 4.32LLDD187 pKa = 4.32GGYY190 pKa = 10.99GDD192 pKa = 5.95DD193 pKa = 4.15YY194 pKa = 11.85LNGGSGADD202 pKa = 3.17TYY204 pKa = 11.87VFGACYY210 pKa = 10.23GDD212 pKa = 6.11DD213 pKa = 3.93VIEE216 pKa = 4.77DD217 pKa = 4.04CGDD220 pKa = 3.51PCEE223 pKa = 4.17TDD225 pKa = 3.47VVEE228 pKa = 5.63FKK230 pKa = 11.24SDD232 pKa = 3.12INLSDD237 pKa = 3.59VCFTACGCDD246 pKa = 3.63LLVTAGKK253 pKa = 10.42GDD255 pKa = 4.37SITIKK260 pKa = 10.6DD261 pKa = 3.73WFAGSQNQIEE271 pKa = 4.23EE272 pKa = 4.42FKK274 pKa = 10.71FCEE277 pKa = 4.2GTITNTQINDD287 pKa = 3.54ALVCYY292 pKa = 8.79DD293 pKa = 4.03TVCAPSLIQLSEE305 pKa = 3.85EE306 pKa = 3.87AMGANVIGG314 pKa = 3.86

Molecular weight: 33.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G4CNS6|G4CNS6_9NEIS DNA-directed RNA polymerase subunit alpha OS=Neisseria wadsworthii 9715 OX=1030841 GN=rpoA PE=3 SV=1
MM1 pKa = 7.41ARR3 pKa = 11.84IAGVNIPNNSHH14 pKa = 6.45IVIGLQAIYY23 pKa = 10.36GIGSTRR29 pKa = 11.84AKK31 pKa = 10.13LICEE35 pKa = 4.06AAGVPASTKK44 pKa = 10.6VKK46 pKa = 10.77DD47 pKa = 3.76LDD49 pKa = 3.82EE50 pKa = 4.64TQLEE54 pKa = 4.22ALRR57 pKa = 11.84EE58 pKa = 4.01QVGKK62 pKa = 10.68YY63 pKa = 9.51EE64 pKa = 4.03IEE66 pKa = 4.43GDD68 pKa = 3.46LRR70 pKa = 11.84RR71 pKa = 11.84EE72 pKa = 3.78VTMSIKK78 pKa = 10.52RR79 pKa = 11.84LMDD82 pKa = 3.27MGCYY86 pKa = 9.44RR87 pKa = 11.84GFRR90 pKa = 11.84HH91 pKa = 6.6RR92 pKa = 11.84RR93 pKa = 11.84GLPCRR98 pKa = 11.84GQRR101 pKa = 11.84TRR103 pKa = 11.84TNARR107 pKa = 11.84TRR109 pKa = 11.84KK110 pKa = 9.46GPRR113 pKa = 11.84KK114 pKa = 9.99AIAGKK119 pKa = 10.13KK120 pKa = 9.03

Molecular weight: 13.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2553

0

2553

700031

39

3837

274.2

30.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.833 ± 0.075

1.051 ± 0.019

5.123 ± 0.039

6.199 ± 0.056

4.143 ± 0.038

7.311 ± 0.069

2.236 ± 0.029

5.959 ± 0.04

5.552 ± 0.045

10.088 ± 0.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.621 ± 0.027

4.308 ± 0.064

4.204 ± 0.038

4.346 ± 0.043

5.072 ± 0.048

5.71 ± 0.042

5.131 ± 0.044

6.808 ± 0.049

1.274 ± 0.023

3.033 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski