Pythium polare RNA virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; unclassified Totiviridae

Average proteome isoelectric point is 7.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z6C4F3|A0A2Z6C4F3_9VIRU RNA-directed RNA polymerase OS=Pythium polare RNA virus 1 OX=2137353 GN=RdRp PE=4 SV=1
MM1 pKa = 8.2DD2 pKa = 4.45SQSSTNSPAEE12 pKa = 3.78DD13 pKa = 3.32LKK15 pKa = 10.74ISKK18 pKa = 10.25SEE20 pKa = 3.98GVGSVRR26 pKa = 11.84VDD28 pKa = 3.23GVFDD32 pKa = 4.17FFVEE36 pKa = 4.43AMPEE40 pKa = 3.86IALSVADD47 pKa = 4.24LQGSAMISHH56 pKa = 7.22LVSDD60 pKa = 3.85QRR62 pKa = 11.84MAAIAAADD70 pKa = 3.69QVFLSAGSGAISPFVRR86 pKa = 11.84SARR89 pKa = 11.84TITQSGSIAVLPVATPTLVGGARR112 pKa = 11.84AAPWNWPFRR121 pKa = 11.84RR122 pKa = 11.84VPSTNVQSTAFLTKK136 pKa = 10.3VDD138 pKa = 3.61VARR141 pKa = 11.84AAEE144 pKa = 4.18KK145 pKa = 10.79SGFDD149 pKa = 3.25AGYY152 pKa = 10.42QKK154 pKa = 10.35FRR156 pKa = 11.84RR157 pKa = 11.84YY158 pKa = 10.93DD159 pKa = 3.39NDD161 pKa = 3.49GLLGLIPSILLEE173 pKa = 4.3QPHH176 pKa = 6.7LWLSWIRR183 pKa = 11.84EE184 pKa = 4.13LSCRR188 pKa = 11.84LAFEE192 pKa = 4.51EE193 pKa = 5.1RR194 pKa = 11.84DD195 pKa = 3.95AQEE198 pKa = 4.02SAALSTMTLPSADD211 pKa = 2.83GVHH214 pKa = 6.45PVNGAVAGLLGDD226 pKa = 4.45ANPAFFGRR234 pKa = 11.84SVGRR238 pKa = 11.84MSEE241 pKa = 3.89ASSLNWEE248 pKa = 4.29EE249 pKa = 3.81GLAVHH254 pKa = 6.62LQQPAGAAAVVCPEE268 pKa = 4.32VTEE271 pKa = 4.51SWCSVVTRR279 pKa = 11.84GRR281 pKa = 11.84IPAWIEE287 pKa = 3.23QAQAGNLSAEE297 pKa = 4.45VFVSRR302 pKa = 11.84ARR304 pKa = 11.84SPRR307 pKa = 11.84QLLGDD312 pKa = 4.02MAAAWAEE319 pKa = 4.08RR320 pKa = 11.84SRR322 pKa = 11.84SGSFFDD328 pKa = 3.21QRR330 pKa = 11.84GRR332 pKa = 11.84MWVLAPHH339 pKa = 6.56VDD341 pKa = 3.77LEE343 pKa = 4.45EE344 pKa = 4.03KK345 pKa = 10.62RR346 pKa = 11.84KK347 pKa = 9.57FLDD350 pKa = 3.36FGGVHH355 pKa = 5.82IHH357 pKa = 5.6PTRR360 pKa = 11.84HH361 pKa = 5.61YY362 pKa = 8.43SWSVISQAVASGGPQFPALPGTSRR386 pKa = 11.84DD387 pKa = 3.08VLAIVRR393 pKa = 11.84HH394 pKa = 5.41LACEE398 pKa = 4.07RR399 pKa = 11.84VTGTLPGAGDD409 pKa = 3.64QGGHH413 pKa = 5.73NPWDD417 pKa = 3.71TALGSSPWQVRR428 pKa = 11.84LAAALGFVEE437 pKa = 5.22EE438 pKa = 4.26MNAAKK443 pKa = 10.32GSYY446 pKa = 9.45LPSGGPLTNILAFEE460 pKa = 4.36GVRR463 pKa = 11.84LGRR466 pKa = 11.84GFDD469 pKa = 3.6RR470 pKa = 11.84VWQSVCGCPASLAGWRR486 pKa = 11.84GRR488 pKa = 11.84GDD490 pKa = 4.0ALWAAGLASVNCLYY504 pKa = 10.88GSSGAGKK511 pKa = 8.97PLGVFFTSDD520 pKa = 3.55APDD523 pKa = 5.42GIASAPDD530 pKa = 3.17IGPVTVYY537 pKa = 10.66ARR539 pKa = 11.84QRR541 pKa = 11.84WGSYY545 pKa = 7.96GWSANVPSEE554 pKa = 3.92WGSLDD559 pKa = 3.44AQGSKK564 pKa = 9.18TFTLDD569 pKa = 4.12HH570 pKa = 6.31GNFWEE575 pKa = 5.17GGSLDD580 pKa = 3.82SRR582 pKa = 11.84SLSWAMLLTHH592 pKa = 6.75DD593 pKa = 3.8EE594 pKa = 4.35RR595 pKa = 11.84GTVRR599 pKa = 11.84MDD601 pKa = 2.7ARR603 pKa = 11.84MVGVQMTRR611 pKa = 11.84VDD613 pKa = 3.41LRR615 pKa = 11.84LRR617 pKa = 11.84GPQAHH622 pKa = 6.61RR623 pKa = 11.84RR624 pKa = 11.84GAHH627 pKa = 6.78DD628 pKa = 3.6SFEE631 pKa = 4.49VPVFWVKK638 pKa = 10.94NSFRR642 pKa = 11.84CWASTAWLNVADD654 pKa = 5.83GIRR657 pKa = 11.84SFAGVYY663 pKa = 6.07TTEE666 pKa = 3.84GLGAVRR672 pKa = 11.84GPGASCVSGGLAFF685 pKa = 5.02

Molecular weight: 73.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z6C4F3|A0A2Z6C4F3_9VIRU RNA-directed RNA polymerase OS=Pythium polare RNA virus 1 OX=2137353 GN=RdRp PE=4 SV=1
MM1 pKa = 7.2KK2 pKa = 10.2RR3 pKa = 11.84CGASGMLRR11 pKa = 11.84RR12 pKa = 11.84LGMVSGKK19 pKa = 9.8DD20 pKa = 3.45HH21 pKa = 6.78SCDD24 pKa = 3.15GSGAPEE30 pKa = 4.34FSDD33 pKa = 4.96KK34 pKa = 11.12DD35 pKa = 3.61LSLWKK40 pKa = 10.48AVGFRR45 pKa = 11.84GRR47 pKa = 11.84KK48 pKa = 5.89TAGTQRR54 pKa = 11.84LGGKK58 pKa = 8.17WRR60 pKa = 11.84TILNRR65 pKa = 11.84YY66 pKa = 9.0RR67 pKa = 11.84GVIPASALSYY77 pKa = 10.66FFTIVNKK84 pKa = 10.54CSVCCEE90 pKa = 3.65AGIIEE95 pKa = 4.16WLKK98 pKa = 10.68EE99 pKa = 3.95FGRR102 pKa = 11.84VCTAATVHH110 pKa = 6.13VGHH113 pKa = 6.75GPWTYY118 pKa = 10.16FCDD121 pKa = 3.58VHH123 pKa = 8.12VLGGYY128 pKa = 7.7DD129 pKa = 3.24TVLNRR134 pKa = 11.84VDD136 pKa = 3.25VMANIRR142 pKa = 11.84DD143 pKa = 4.18SVGLPLVSGLSDD155 pKa = 3.64YY156 pKa = 11.63EE157 pKa = 4.12DD158 pKa = 3.56VLRR161 pKa = 11.84GHH163 pKa = 6.54VRR165 pKa = 11.84DD166 pKa = 3.28ALRR169 pKa = 11.84NVRR172 pKa = 11.84VDD174 pKa = 2.71SGMDD178 pKa = 3.0VAFADD183 pKa = 3.64WVRR186 pKa = 11.84FRR188 pKa = 11.84DD189 pKa = 3.35NWTVPGACNIGKK201 pKa = 9.06HH202 pKa = 6.12AILEE206 pKa = 4.03VRR208 pKa = 11.84RR209 pKa = 11.84PGRR212 pKa = 11.84GVKK215 pKa = 9.5RR216 pKa = 11.84AKK218 pKa = 10.67AGGKK222 pKa = 9.21LGKK225 pKa = 7.73TLCLSDD231 pKa = 3.73QEE233 pKa = 4.49LVRR236 pKa = 11.84LATEE240 pKa = 3.87RR241 pKa = 11.84CGAQILPFRR250 pKa = 11.84KK251 pKa = 9.05EE252 pKa = 3.93DD253 pKa = 3.66EE254 pKa = 4.25PVKK257 pKa = 9.67TRR259 pKa = 11.84VVFGYY264 pKa = 7.57DD265 pKa = 2.67TRR267 pKa = 11.84SYY269 pKa = 10.79LRR271 pKa = 11.84CSYY274 pKa = 11.59ADD276 pKa = 3.55AFLGDD281 pKa = 4.24LNANGSWTPLGCSVADD297 pKa = 4.07RR298 pKa = 11.84AQQRR302 pKa = 11.84IDD304 pKa = 2.11IWTRR308 pKa = 11.84LGGSAEE314 pKa = 4.08RR315 pKa = 11.84AVSLDD320 pKa = 3.22QSSFDD325 pKa = 4.03LNQPKK330 pKa = 9.2WAVRR334 pKa = 11.84MAIEE338 pKa = 4.14EE339 pKa = 4.24VFNRR343 pKa = 11.84IIDD346 pKa = 3.95CCHH349 pKa = 6.64PEE351 pKa = 4.07LVEE354 pKa = 3.96EE355 pKa = 4.22VTKK358 pKa = 10.91FKK360 pKa = 10.68EE361 pKa = 4.07LEE363 pKa = 3.92LFAFDD368 pKa = 3.61EE369 pKa = 4.36ARR371 pKa = 11.84VGDD374 pKa = 3.59VCMWGRR380 pKa = 11.84GVPSGHH386 pKa = 6.81KK387 pKa = 7.89WTALVDD393 pKa = 3.58TLLNRR398 pKa = 11.84GEE400 pKa = 4.24CLIAAEE406 pKa = 4.06LRR408 pKa = 11.84GVEE411 pKa = 4.38VVRR414 pKa = 11.84GLWQGDD420 pKa = 3.54DD421 pKa = 3.44GLVFEE426 pKa = 5.19KK427 pKa = 11.05GKK429 pKa = 9.34ATMSWADD436 pKa = 3.55AYY438 pKa = 10.97DD439 pKa = 3.56KK440 pKa = 11.41LGLVVNAAKK449 pKa = 9.21TWVDD453 pKa = 3.29STSCEE458 pKa = 4.1FLHH461 pKa = 6.35EE462 pKa = 4.92FYY464 pKa = 10.77SASGVRR470 pKa = 11.84AFPARR475 pKa = 11.84AFRR478 pKa = 11.84SVCWDD483 pKa = 3.54KK484 pKa = 11.32PVMGASSFLSGTEE497 pKa = 3.89RR498 pKa = 11.84LNSRR502 pKa = 11.84LDD504 pKa = 3.52VLLKK508 pKa = 9.63CARR511 pKa = 11.84RR512 pKa = 11.84GLYY515 pKa = 10.26RR516 pKa = 11.84MADD519 pKa = 3.34EE520 pKa = 4.7AVRR523 pKa = 11.84MLTKK527 pKa = 10.37RR528 pKa = 11.84GLSAVHH534 pKa = 6.35AKK536 pKa = 9.84EE537 pKa = 4.05VLCTSRR543 pKa = 11.84NLGGLGWSDD552 pKa = 3.08SMRR555 pKa = 11.84RR556 pKa = 11.84ALVVKK561 pKa = 10.23GSEE564 pKa = 3.68VEE566 pKa = 4.03YY567 pKa = 9.77RR568 pKa = 11.84HH569 pKa = 5.62VSIVSPVFGSGSSGCFSFGALRR591 pKa = 11.84RR592 pKa = 11.84LGAHH596 pKa = 5.86MPLPVSEE603 pKa = 4.92LVVSSRR609 pKa = 11.84WVSPVAEE616 pKa = 4.4VPVHH620 pKa = 6.85DD621 pKa = 4.8RR622 pKa = 11.84LGRR625 pKa = 11.84DD626 pKa = 2.93TKK628 pKa = 11.16LRR630 pKa = 11.84LNWNFRR636 pKa = 11.84DD637 pKa = 3.89PVSDD641 pKa = 3.22PWRR644 pKa = 11.84RR645 pKa = 11.84RR646 pKa = 11.84LEE648 pKa = 4.17MEE650 pKa = 3.5WMLARR655 pKa = 11.84GRR657 pKa = 11.84DD658 pKa = 3.21WCDD661 pKa = 2.86ALVPDD666 pKa = 4.7EE667 pKa = 4.6CCRR670 pKa = 11.84TSPLGAEE677 pKa = 3.89RR678 pKa = 11.84AFRR681 pKa = 11.84FASRR685 pKa = 11.84WASEE689 pKa = 3.97RR690 pKa = 11.84LNLDD694 pKa = 3.41CEE696 pKa = 4.54LTTGEE701 pKa = 4.44SWCVLADD708 pKa = 3.98LGNRR712 pKa = 11.84MWMGLVACMVGSGNVMGMRR731 pKa = 11.84KK732 pKa = 8.7IDD734 pKa = 4.08GYY736 pKa = 10.52WLGLYY741 pKa = 9.68RR742 pKa = 11.84RR743 pKa = 11.84IWRR746 pKa = 11.84YY747 pKa = 8.81QVLSKK752 pKa = 10.57PLFKK756 pKa = 10.48IRR758 pKa = 11.84VV759 pKa = 3.58

Molecular weight: 84.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1444

685

759

722.0

78.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.042 ± 1.573

2.493 ± 0.784

5.609 ± 0.429

4.709 ± 0.316

4.086 ± 0.195

10.111 ± 0.46

1.87 ± 0.116

2.632 ± 0.094

2.978 ± 0.912

9.141 ± 0.545

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.285 ± 0.258

2.493 ± 0.105

4.155 ± 0.731

2.285 ± 0.81

8.657 ± 0.999

8.587 ± 0.987

3.878 ± 0.139

9.003 ± 0.453

3.393 ± 0.024

1.593 ± 0.282
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski