Caulobacter phage phiCb5

Taxonomy: Viruses; Riboviria; Orthornavirae; Lenarviricota; Leviviricetes; Timlovirales; Steitzviridae; Cebevirus; Cebevirus halophobicum

Average proteome isoelectric point is 8.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D7RIC3|D7RIC3_9VIRU RNA replicase beta chain OS=Caulobacter phage phiCb5 OX=767473 PE=4 SV=1
MM1 pKa = 7.68ALGDD5 pKa = 4.09TLTITLGGSGGTAKK19 pKa = 10.51VLRR22 pKa = 11.84KK23 pKa = 9.56INQDD27 pKa = 3.2GYY29 pKa = 8.25TSEE32 pKa = 4.79YY33 pKa = 9.95YY34 pKa = 10.77LPEE37 pKa = 4.16TSSSFRR43 pKa = 11.84AKK45 pKa = 9.92VRR47 pKa = 11.84HH48 pKa = 5.31TKK50 pKa = 10.66EE51 pKa = 4.03SVKK54 pKa = 10.26PNQVQYY60 pKa = 10.52EE61 pKa = 4.09RR62 pKa = 11.84HH63 pKa = 5.04NVEE66 pKa = 3.87FTEE69 pKa = 4.41TVYY72 pKa = 11.39ASGSTPEE79 pKa = 4.4FVRR82 pKa = 11.84QAYY85 pKa = 8.94VVIRR89 pKa = 11.84HH90 pKa = 5.51KK91 pKa = 11.22VGDD94 pKa = 3.65VSATVSDD101 pKa = 3.86LGEE104 pKa = 4.09ALSFYY109 pKa = 10.82LNEE112 pKa = 4.02ALYY115 pKa = 10.64GKK117 pKa = 10.57LIGWEE122 pKa = 4.15SS123 pKa = 3.11

Molecular weight: 13.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D7RIC4|D7RIC4_9VIRU Putative lysis protein OS=Caulobacter phage phiCb5 OX=767473 PE=4 SV=1
MM1 pKa = 7.9KK2 pKa = 10.17IPQEE6 pKa = 4.11VLTLCLIVFSGLLTLFLASLGLLILEE32 pKa = 4.46KK33 pKa = 10.6ALRR36 pKa = 11.84STDD39 pKa = 3.56LVPFQTSVGIKK50 pKa = 9.34IVSISSRR57 pKa = 11.84PGLKK61 pKa = 9.72SSRR64 pKa = 11.84VSFQLMSGVITPTRR78 pKa = 11.84HH79 pKa = 6.33GSTTCSGFRR88 pKa = 11.84GSICLLGMSLQVSSLLYY105 pKa = 9.69RR106 pKa = 11.84RR107 pKa = 11.84RR108 pKa = 11.84RR109 pKa = 11.84SLRR112 pKa = 11.84DD113 pKa = 3.22SSPPSLPPISTVNKK127 pKa = 10.14ISDD130 pKa = 3.64HH131 pKa = 5.77GSKK134 pKa = 9.38TEE136 pKa = 3.67

Molecular weight: 14.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1286

123

655

321.5

35.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.599 ± 0.847

1.4 ± 0.266

5.288 ± 1.253

3.733 ± 0.651

4.121 ± 0.405

7.543 ± 0.861

2.255 ± 0.181

5.132 ± 0.898

4.821 ± 0.319

10.653 ± 1.093

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.555 ± 0.187

3.344 ± 0.713

5.288 ± 0.441

2.955 ± 0.137

7.465 ± 0.582

11.12 ± 1.377

6.454 ± 0.78

6.454 ± 0.659

2.022 ± 0.598

2.799 ± 0.742
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski