Candidatus Gracilibacteria bacterium

Taxonomy: cellular organisms; Bacteria; Bacteria incertae sedis; Bacteria candidate phyla; Patescibacteria group; Candidatus Gracilibacteria; unclassified Candidatus Gracilibacteria

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1051 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2W4K1E4|A0A2W4K1E4_9BACT Uncharacterized protein OS=Candidatus Gracilibacteria bacterium OX=2044595 GN=DLH72_04315 PE=4 SV=1
MM1 pKa = 7.53LFYY4 pKa = 10.79RR5 pKa = 11.84GGNYY9 pKa = 9.36YY10 pKa = 10.29RR11 pKa = 11.84GSDD14 pKa = 3.87CVVLVYY20 pKa = 9.37FLNFQTMGMYY30 pKa = 10.31VSYY33 pKa = 9.45YY34 pKa = 10.29TLDD37 pKa = 3.89DD38 pKa = 3.79EE39 pKa = 4.65QFAEE43 pKa = 4.26IGSMNDD49 pKa = 3.0DD50 pKa = 4.56ALYY53 pKa = 10.85DD54 pKa = 3.82WIEE57 pKa = 4.15EE58 pKa = 3.96QDD60 pKa = 3.8EE61 pKa = 4.5DD62 pKa = 5.22DD63 pKa = 5.65DD64 pKa = 7.04AITTDD69 pKa = 5.22IDD71 pKa = 3.87KK72 pKa = 10.79AWQGLSYY79 pKa = 10.77ILTGSDD85 pKa = 3.6FSDD88 pKa = 3.84GSLIGKK94 pKa = 9.34ALFGEE99 pKa = 4.71TVVNEE104 pKa = 4.26DD105 pKa = 3.9EE106 pKa = 4.49YY107 pKa = 11.35MAITPKK113 pKa = 10.6EE114 pKa = 4.14SIAAIVEE121 pKa = 3.99ALDD124 pKa = 4.1AVDD127 pKa = 3.88IKK129 pKa = 11.46SKK131 pKa = 10.4IAEE134 pKa = 3.93ADD136 pKa = 3.39FAEE139 pKa = 4.49MNRR142 pKa = 11.84RR143 pKa = 11.84DD144 pKa = 3.77IYY146 pKa = 9.79PAIWEE151 pKa = 4.26EE152 pKa = 3.72NDD154 pKa = 3.61RR155 pKa = 11.84EE156 pKa = 4.35DD157 pKa = 3.44VTEE160 pKa = 3.89YY161 pKa = 11.42LLDD164 pKa = 3.83NFSSLKK170 pKa = 9.81EE171 pKa = 4.08FYY173 pKa = 10.6RR174 pKa = 11.84EE175 pKa = 4.0AQEE178 pKa = 3.71KK179 pKa = 9.18HH180 pKa = 6.54LNVLVLLGG188 pKa = 3.76

Molecular weight: 21.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2W4IS11|A0A2W4IS11_9BACT Uncharacterized protein OS=Candidatus Gracilibacteria bacterium OX=2044595 GN=DLH72_03545 PE=4 SV=1
MM1 pKa = 7.36ARR3 pKa = 11.84KK4 pKa = 9.69AKK6 pKa = 9.6IAKK9 pKa = 9.57ANKK12 pKa = 8.53LKK14 pKa = 10.8KK15 pKa = 10.13RR16 pKa = 11.84FLQSITNGKK25 pKa = 8.87KK26 pKa = 9.68PKK28 pKa = 10.12FATKK32 pKa = 10.31VFNCCSVCGRR42 pKa = 11.84TRR44 pKa = 11.84GYY46 pKa = 9.85LGKK49 pKa = 10.81YY50 pKa = 7.95GICRR54 pKa = 11.84ICFRR58 pKa = 11.84EE59 pKa = 3.96KK60 pKa = 11.02ANAGEE65 pKa = 4.1LPGVRR70 pKa = 11.84KK71 pKa = 10.36SSWW74 pKa = 2.83

Molecular weight: 8.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1051

0

1051

298787

25

4219

284.3

32.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.851 ± 0.069

0.688 ± 0.025

5.498 ± 0.074

8.23 ± 0.094

6.266 ± 0.094

6.161 ± 0.08

1.069 ± 0.024

10.351 ± 0.11

11.18 ± 0.11

9.052 ± 0.08

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.814 ± 0.04

7.258 ± 0.102

2.316 ± 0.049

2.392 ± 0.041

2.863 ± 0.053

6.688 ± 0.079

4.499 ± 0.069

4.969 ± 0.069

0.582 ± 0.018

4.274 ± 0.054
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski