Shewanella phage X14

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 39 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8NE39|A0A4P8NE39_9CAUD Coil containing protein OS=Shewanella phage X14 OX=2576871 PE=4 SV=1
MM1 pKa = 7.58QYY3 pKa = 9.98TKK5 pKa = 10.5TVTLDD10 pKa = 3.18INGSEE15 pKa = 3.65IHH17 pKa = 6.93AEE19 pKa = 4.16LKK21 pKa = 10.71FEE23 pKa = 4.23YY24 pKa = 9.7TPEE27 pKa = 3.94IPGVYY32 pKa = 8.64TALPEE37 pKa = 3.94NSYY40 pKa = 8.73PTEE43 pKa = 4.14PEE45 pKa = 3.92EE46 pKa = 4.92FDD48 pKa = 4.11LLSLEE53 pKa = 4.57TEE55 pKa = 5.01DD56 pKa = 5.9GDD58 pKa = 3.77NCDD61 pKa = 3.76WIIPYY66 pKa = 9.79IAEE69 pKa = 4.53DD70 pKa = 3.63LVSQLKK76 pKa = 10.41EE77 pKa = 4.38GEE79 pKa = 4.25DD80 pKa = 3.78SLL82 pKa = 5.27

Molecular weight: 9.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8NFH1|A0A4P8NFH1_9CAUD Coil containing protein OS=Shewanella phage X14 OX=2576871 PE=4 SV=1
MM1 pKa = 7.19ITQSQLKK8 pKa = 9.88KK9 pKa = 10.35LLHH12 pKa = 6.41YY13 pKa = 10.04DD14 pKa = 3.4PKK16 pKa = 11.14SGVFTWKK23 pKa = 9.84PRR25 pKa = 11.84ALKK28 pKa = 10.11QFKK31 pKa = 9.0TKK33 pKa = 10.3RR34 pKa = 11.84AFSTWTAKK42 pKa = 10.6HH43 pKa = 5.3QDD45 pKa = 3.78KK46 pKa = 10.85IAGSINYY53 pKa = 9.14FGTKK57 pKa = 9.51KK58 pKa = 10.61YY59 pKa = 10.92ISIKK63 pKa = 10.25PLGEE67 pKa = 3.46YY68 pKa = 10.43HH69 pKa = 6.99LAHH72 pKa = 6.85RR73 pKa = 11.84LAWLYY78 pKa = 7.39MTGSFPKK85 pKa = 10.15EE86 pKa = 4.09QIDD89 pKa = 3.88HH90 pKa = 6.36VDD92 pKa = 3.57GNGLNNAFANLRR104 pKa = 11.84SVTQKK109 pKa = 10.88EE110 pKa = 3.92NAKK113 pKa = 9.87NLRR116 pKa = 11.84MYY118 pKa = 10.88KK119 pKa = 10.45NNTSGFTGVAWRR131 pKa = 11.84KK132 pKa = 9.57KK133 pKa = 8.33IKK135 pKa = 9.73KK136 pKa = 6.72WQANIRR142 pKa = 11.84VNKK145 pKa = 9.46KK146 pKa = 10.13NIYY149 pKa = 10.33LGVFHH154 pKa = 7.71DD155 pKa = 4.56LADD158 pKa = 4.85AIKK161 pKa = 10.28ARR163 pKa = 11.84KK164 pKa = 8.01EE165 pKa = 3.85AEE167 pKa = 4.03FKK169 pKa = 11.18YY170 pKa = 10.45GFHH173 pKa = 7.01KK174 pKa = 10.98NHH176 pKa = 6.76GGSLL180 pKa = 3.54

Molecular weight: 20.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

39

0

39

10943

44

2740

280.6

31.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.955 ± 0.444

0.905 ± 0.295

6.004 ± 0.228

8.681 ± 0.586

3.701 ± 0.325

6.744 ± 0.431

1.554 ± 0.221

5.428 ± 0.218

8.224 ± 0.415

7.493 ± 0.312

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.787 ± 0.124

4.505 ± 0.312

3.829 ± 0.293

5.227 ± 0.338

4.606 ± 0.249

5.647 ± 0.277

5.227 ± 0.324

6.031 ± 0.272

1.206 ± 0.18

3.244 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski