Candidatus Methylomirabilis lanthanidiphila

Taxonomy: cellular organisms; Bacteria; Bacteria incertae sedis; Bacteria candidate phyla; candidate division NC10; Candidatus Methylomirabilis

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3012 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A564ZLL1|A0A564ZLL1_9BACT Uncharacterized protein OS=Candidatus Methylomirabilis lanthanidiphila OX=2211376 GN=MELA_01822 PE=4 SV=1
MM1 pKa = 7.68KK2 pKa = 9.15MQKK5 pKa = 9.92VLLTLCGAVLLVFLMTAPVLAAHH28 pKa = 7.14CSPEE32 pKa = 4.33FVGPPTSGCTVNKK45 pKa = 9.77VQDD48 pKa = 3.96VLCQGTDD55 pKa = 3.42GNDD58 pKa = 3.8TIVGTSGDD66 pKa = 3.92DD67 pKa = 3.67VIIGLGGNDD76 pKa = 3.81RR77 pKa = 11.84IYY79 pKa = 11.31GLGGDD84 pKa = 4.58DD85 pKa = 4.57VICGRR90 pKa = 11.84DD91 pKa = 3.3GDD93 pKa = 4.77DD94 pKa = 3.62ILVGGAGDD102 pKa = 4.79DD103 pKa = 4.78LIEE106 pKa = 5.78ADD108 pKa = 4.25GGDD111 pKa = 3.45DD112 pKa = 4.32RR113 pKa = 11.84IYY115 pKa = 11.06GGSGDD120 pKa = 4.62DD121 pKa = 4.93LLDD124 pKa = 4.23GGDD127 pKa = 4.84DD128 pKa = 3.68FDD130 pKa = 6.64IINGGPGFEE139 pKa = 4.37VACVDD144 pKa = 3.65GEE146 pKa = 4.34RR147 pKa = 11.84VNNCEE152 pKa = 3.73

Molecular weight: 15.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A564ZGH0|A0A564ZGH0_9BACT Ribonuclease VapC OS=Candidatus Methylomirabilis lanthanidiphila OX=2211376 GN=vapC_1 PE=3 SV=1
RR1 pKa = 7.62RR2 pKa = 11.84NALMKK7 pKa = 10.55KK8 pKa = 8.8GLKK11 pKa = 9.93RR12 pKa = 11.84GLANPLHH19 pKa = 5.19TVKK22 pKa = 11.09NNVRR26 pKa = 11.84RR27 pKa = 11.84SALTKK32 pKa = 10.19KK33 pKa = 10.16GLKK36 pKa = 9.38RR37 pKa = 11.84RR38 pKa = 11.84PGAGGTRR45 pKa = 11.84AGKK48 pKa = 10.01

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3012

0

3012

889585

21

2520

295.3

32.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.164 ± 0.05

1.059 ± 0.016

5.113 ± 0.026

6.352 ± 0.044

3.63 ± 0.029

8.082 ± 0.048

2.246 ± 0.02

5.572 ± 0.035

3.709 ± 0.037

10.847 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.2 ± 0.019

2.462 ± 0.025

5.057 ± 0.036

3.516 ± 0.021

7.691 ± 0.046

5.34 ± 0.03

5.421 ± 0.036

7.66 ± 0.036

1.277 ± 0.021

2.6 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski