Microbacterium phage Ixel

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Neferthenavirus; unclassified Neferthenavirus

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M9Z421|A0A6M9Z421_9CAUD Minor tail protein OS=Microbacterium phage Ixel OX=2736269 GN=19 PE=4 SV=1
MM1 pKa = 7.75AAIDD5 pKa = 4.61DD6 pKa = 4.58LNGLLSPLPGYY17 pKa = 10.68GILTDD22 pKa = 3.74VMKK25 pKa = 9.77QTALDD30 pKa = 3.78GARR33 pKa = 11.84IPDD36 pKa = 4.04GAGVWPGQPGYY47 pKa = 8.84QTTYY51 pKa = 10.51DD52 pKa = 3.95IYY54 pKa = 10.24WAALSLIGFLQAQPVVRR71 pKa = 11.84QTSSEE76 pKa = 4.15GTSVAVDD83 pKa = 3.45APNWTGIAAYY93 pKa = 10.16YY94 pKa = 10.19RR95 pKa = 11.84GASVIAGAASASPILNVIPIPGGPHH120 pKa = 5.11VVPVDD125 pKa = 3.18MSGRR129 pKa = 11.84GGEE132 pKa = 4.47PYY134 pKa = 10.9DD135 pKa = 5.73DD136 pKa = 4.59VDD138 pKa = 3.5TDD140 pKa = 3.81TAA142 pKa = 4.01

Molecular weight: 14.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M9Z4S1|A0A6M9Z4S1_9CAUD Portal protein OS=Microbacterium phage Ixel OX=2736269 GN=5 PE=4 SV=1
MM1 pKa = 7.12QNSSPVALEE10 pKa = 3.8LQRR13 pKa = 11.84LLRR16 pKa = 11.84PTACSFRR23 pKa = 11.84EE24 pKa = 3.73KK25 pKa = 9.69HH26 pKa = 5.73TYY28 pKa = 9.68QIDD31 pKa = 3.47RR32 pKa = 11.84RR33 pKa = 11.84IHH35 pKa = 5.82GGVILICKK43 pKa = 7.41WTLCQQRR50 pKa = 11.84KK51 pKa = 6.95VVSNAVLRR59 pKa = 11.84MMVARR64 pKa = 5.03

Molecular weight: 7.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

12932

48

812

215.5

23.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.254 ± 0.447

0.619 ± 0.09

5.885 ± 0.401

6.766 ± 0.564

2.768 ± 0.191

7.996 ± 0.346

1.987 ± 0.169

5.189 ± 0.391

4.686 ± 0.436

8.552 ± 0.343

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.366 ± 0.193

3.17 ± 0.227

5.266 ± 0.29

4.384 ± 0.243

5.56 ± 0.364

5.908 ± 0.332

6.627 ± 0.439

7.439 ± 0.294

1.918 ± 0.173

2.66 ± 0.214
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski