Escherichia coli R178

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia; Escherichia coli

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 270 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M5DRK1|A0A0M5DRK1_ECOLX Uncharacterized protein OS=Escherichia coli R178 OX=1408252 PE=4 SV=1
MM1 pKa = 8.15DD2 pKa = 6.05DD3 pKa = 5.04KK4 pKa = 11.57EE5 pKa = 4.28QFTNLVAKK13 pKa = 9.73HH14 pKa = 5.96ASGLTEE20 pKa = 4.05EE21 pKa = 4.45QLAGYY26 pKa = 7.55DD27 pKa = 3.41ACSLDD32 pKa = 4.18GEE34 pKa = 5.0CVTPSYY40 pKa = 11.0EE41 pKa = 3.81VFRR44 pKa = 11.84GYY46 pKa = 8.63RR47 pKa = 11.84TRR49 pKa = 11.84HH50 pKa = 4.99TLDD53 pKa = 3.52EE54 pKa = 4.44FLEE57 pKa = 4.34MAISLNAIHH66 pKa = 7.31PDD68 pKa = 3.29EE69 pKa = 4.95YY70 pKa = 10.71LTDD73 pKa = 3.71MLLKK77 pKa = 8.31PHH79 pKa = 6.09EE80 pKa = 4.8VIGALADD87 pKa = 4.65EE88 pKa = 5.09GDD90 pKa = 3.98QLNNATPVYY99 pKa = 9.0FFPDD103 pKa = 3.14TGVYY107 pKa = 9.56AAAVSEE113 pKa = 4.13TRR115 pKa = 11.84VLDD118 pKa = 4.6AWLCWPCYY126 pKa = 7.48PANWW130 pKa = 3.63

Molecular weight: 14.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M5X0M8|A0A0M5X0M8_ECOLX Uncharacterized protein OS=Escherichia coli R178 OX=1408252 PE=4 SV=1
MM1 pKa = 7.57ASVSISCPSCSATDD15 pKa = 3.7GVVRR19 pKa = 11.84NGKK22 pKa = 8.27STAGHH27 pKa = 4.88QRR29 pKa = 11.84YY30 pKa = 8.94LCSHH34 pKa = 6.46CRR36 pKa = 11.84KK37 pKa = 7.49TWQLQFTYY45 pKa = 9.73TASQPGTHH53 pKa = 5.79QKK55 pKa = 10.33IIDD58 pKa = 3.52MAMNGVGCRR67 pKa = 11.84ATARR71 pKa = 11.84IMGVGLNTIFRR82 pKa = 11.84HH83 pKa = 5.51LKK85 pKa = 8.99NSGRR89 pKa = 11.84SRR91 pKa = 3.37

Molecular weight: 9.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

270

0

270

61464

15

1434

227.6

25.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.289 ± 0.214

1.25 ± 0.07

5.491 ± 0.121

6.226 ± 0.13

3.901 ± 0.108

6.4 ± 0.141

2.294 ± 0.09

5.685 ± 0.129

5.48 ± 0.154

9.97 ± 0.2

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.699 ± 0.091

4.3 ± 0.135

4.297 ± 0.117

3.763 ± 0.11

5.947 ± 0.2

7.343 ± 0.147

5.584 ± 0.145

6.828 ± 0.127

1.305 ± 0.054

2.948 ± 0.097
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski