Barley yellow striate mosaic cytorhabdovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Betarhabdovirinae; Cytorhabdovirus

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5KH28|A0A0C5KH28_9RHAB M protein OS=Barley yellow striate mosaic cytorhabdovirus OX=1985699 PE=4 SV=1
MM1 pKa = 7.43SSSNAAVTFDD11 pKa = 5.22DD12 pKa = 5.79DD13 pKa = 4.38VPDD16 pKa = 5.73DD17 pKa = 3.95IAPRR21 pKa = 11.84AYY23 pKa = 10.5DD24 pKa = 4.08LDD26 pKa = 4.88LDD28 pKa = 4.85GIFGEE33 pKa = 4.56QDD35 pKa = 3.06VADD38 pKa = 4.73ADD40 pKa = 4.11RR41 pKa = 11.84QAEE44 pKa = 4.08NDD46 pKa = 3.47VEE48 pKa = 4.21EE49 pKa = 4.41QEE51 pKa = 4.94EE52 pKa = 4.46EE53 pKa = 4.22EE54 pKa = 5.15KK55 pKa = 10.36MVPTIKK61 pKa = 10.53GEE63 pKa = 4.02RR64 pKa = 11.84YY65 pKa = 8.37ISGVEE70 pKa = 3.75EE71 pKa = 3.82LAALLKK77 pKa = 10.83DD78 pKa = 3.38HH79 pKa = 7.02ANRR82 pKa = 11.84RR83 pKa = 11.84GMEE86 pKa = 4.03MKK88 pKa = 10.49KK89 pKa = 9.94EE90 pKa = 3.86WVNIMKK96 pKa = 10.4RR97 pKa = 11.84KK98 pKa = 7.26YY99 pKa = 10.37HH100 pKa = 4.93EE101 pKa = 4.89MKK103 pKa = 10.82DD104 pKa = 3.53KK105 pKa = 10.13MLKK108 pKa = 9.62SHH110 pKa = 7.02VEE112 pKa = 3.84LFLLGIQVEE121 pKa = 4.78RR122 pKa = 11.84NTSVDD127 pKa = 3.35KK128 pKa = 11.05DD129 pKa = 4.2FKK131 pKa = 10.87DD132 pKa = 3.06TATRR136 pKa = 11.84LNDD139 pKa = 3.44EE140 pKa = 4.37VNKK143 pKa = 10.31VSGISKK149 pKa = 10.86KK150 pKa = 10.99LMDD153 pKa = 4.45SQAKK157 pKa = 8.53IAKK160 pKa = 10.02DD161 pKa = 3.19VDD163 pKa = 3.55QKK165 pKa = 10.92MKK167 pKa = 10.72EE168 pKa = 3.73LTAYY172 pKa = 8.5CRR174 pKa = 11.84KK175 pKa = 8.25MEE177 pKa = 4.34SMVSEE182 pKa = 4.23VKK184 pKa = 9.99TVVEE188 pKa = 4.35SASRR192 pKa = 11.84PSSIASWAQGEE203 pKa = 4.37EE204 pKa = 4.53KK205 pKa = 9.82NTSEE209 pKa = 5.13DD210 pKa = 3.41YY211 pKa = 11.25NYY213 pKa = 11.32DD214 pKa = 3.38KK215 pKa = 10.44FLSMIGFSANHH226 pKa = 5.95IRR228 pKa = 11.84SSVMKK233 pKa = 8.99KK234 pKa = 9.28CHH236 pKa = 5.38VAITDD241 pKa = 3.15EE242 pKa = 4.73MYY244 pKa = 9.12THH246 pKa = 6.98VISGEE251 pKa = 3.89ADD253 pKa = 3.3SDD255 pKa = 4.3DD256 pKa = 3.49MADD259 pKa = 3.53YY260 pKa = 10.89YY261 pKa = 11.73SRR263 pKa = 11.84ILNYY267 pKa = 10.05ADD269 pKa = 3.79RR270 pKa = 11.84VVKK273 pKa = 10.71GSSKK277 pKa = 10.02EE278 pKa = 3.85EE279 pKa = 3.79RR280 pKa = 11.84TRR282 pKa = 11.84PASSSRR288 pKa = 11.84HH289 pKa = 5.77DD290 pKa = 3.85PYY292 pKa = 11.92GDD294 pKa = 3.36LL295 pKa = 4.72

Molecular weight: 33.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5KQX4|A0A0C5KQX4_9RHAB RNA-directed RNA polymerase L OS=Barley yellow striate mosaic cytorhabdovirus OX=1985699 GN=L PE=3 SV=1
MM1 pKa = 7.55WNSLWLYY8 pKa = 10.65LVDD11 pKa = 4.61GLSQEE16 pKa = 4.31RR17 pKa = 11.84TSSFWATLPFSVQLILAIMILTSLCRR43 pKa = 11.84LKK45 pKa = 10.85VYY47 pKa = 8.02QLKK50 pKa = 10.26YY51 pKa = 10.06FAIISAFQLLAALTSLFRR69 pKa = 11.84EE70 pKa = 4.23LMYY73 pKa = 10.3ATGHH77 pKa = 4.94STT79 pKa = 3.4

Molecular weight: 9.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

3947

51

2056

394.7

44.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.27 ± 0.637

1.596 ± 0.466

5.65 ± 0.575

6.41 ± 0.606

3.851 ± 0.32

5.599 ± 0.402

2.28 ± 0.2

7.17 ± 0.427

6.866 ± 0.403

9.602 ± 0.785

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.344 ± 0.359

4.104 ± 0.341

3.902 ± 0.578

2.711 ± 0.227

5.447 ± 0.26

8.766 ± 0.403

5.726 ± 0.417

6.258 ± 0.277

1.571 ± 0.253

3.876 ± 0.263
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski