Flaviflexus sp. H23T48

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Flaviflexus; unclassified Flaviflexus

Average proteome isoelectric point is 5.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2334 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q9G680|A0A3Q9G680_9ACTO ABC transporter ATP-binding protein OS=Flaviflexus sp. H23T48 OX=2496867 GN=EJ997_01705 PE=4 SV=1
MM1 pKa = 7.26TSHH4 pKa = 6.47VPGSRR9 pKa = 11.84RR10 pKa = 11.84TLRR13 pKa = 11.84ACIATLGAGALGLGAFIVPAAATPSADD40 pKa = 4.13PIYY43 pKa = 10.6EE44 pKa = 4.15SDD46 pKa = 3.54PSVLVGSSANQASVAGSAYY65 pKa = 10.01GSRR68 pKa = 11.84ATGEE72 pKa = 4.08WFVSFSSAPLVNGGTQARR90 pKa = 11.84VDD92 pKa = 3.36ADD94 pKa = 3.99LNEE97 pKa = 4.52LEE99 pKa = 4.87SAIADD104 pKa = 3.57LDD106 pKa = 4.17VEE108 pKa = 5.0VKK110 pKa = 10.61ASHH113 pKa = 6.01SEE115 pKa = 3.69LWAGAVVTADD125 pKa = 3.6DD126 pKa = 4.24AEE128 pKa = 4.33LAKK131 pKa = 11.03VLDD134 pKa = 4.21RR135 pKa = 11.84VDD137 pKa = 3.39VAGVFPVLLIDD148 pKa = 4.75APEE151 pKa = 3.79QRR153 pKa = 11.84DD154 pKa = 3.61LGAEE158 pKa = 3.88PEE160 pKa = 4.08MRR162 pKa = 11.84FAAGMTGADD171 pKa = 3.75FAQDD175 pKa = 3.97LGVSGYY181 pKa = 10.18GQTISIIDD189 pKa = 3.46TGVDD193 pKa = 2.93IDD195 pKa = 4.0HH196 pKa = 6.49VAFGGTGVADD206 pKa = 3.62TTPWSEE212 pKa = 3.82INGNGSRR219 pKa = 11.84IIGGYY224 pKa = 10.58DD225 pKa = 3.71FVGDD229 pKa = 4.72DD230 pKa = 3.86YY231 pKa = 11.65NANPAADD238 pKa = 3.97NYY240 pKa = 10.91NPVPAPDD247 pKa = 4.75SNPDD251 pKa = 3.3DD252 pKa = 4.22CQGHH256 pKa = 5.16GTHH259 pKa = 6.15VAGIAAGSDD268 pKa = 3.64DD269 pKa = 3.63VTGVYY274 pKa = 10.32GVAPDD279 pKa = 4.24ANILAYY285 pKa = 10.02RR286 pKa = 11.84VFGCEE291 pKa = 3.62GSTDD295 pKa = 3.66SAIMLQAMEE304 pKa = 4.23ASARR308 pKa = 11.84DD309 pKa = 3.35GADD312 pKa = 2.78IVNMSIGAGFMTWPNYY328 pKa = 5.66PTAVAADD335 pKa = 3.82NLVDD339 pKa = 3.76AGVVVTVSQGNEE351 pKa = 3.9GEE353 pKa = 4.37SGVFSGGAPAVASKK367 pKa = 10.4VISVGSVDD375 pKa = 3.76NVASEE380 pKa = 4.43APVFVVNDD388 pKa = 3.23QNIAYY393 pKa = 9.52SSSTGAPNPPTDD405 pKa = 3.32NSEE408 pKa = 4.29YY409 pKa = 10.85EE410 pKa = 4.3IIAYY414 pKa = 7.91PAGSEE419 pKa = 4.01TGAVPIEE426 pKa = 4.23DD427 pKa = 4.1AEE429 pKa = 4.49GKK431 pKa = 9.85VVIVRR436 pKa = 11.84RR437 pKa = 11.84GNSTFHH443 pKa = 6.6EE444 pKa = 4.49KK445 pKa = 9.97AAAAQEE451 pKa = 4.21SGAAGVIIDD460 pKa = 4.35NNVAGAINATVEE472 pKa = 4.05GDD474 pKa = 3.58PAITIPTVTISMADD488 pKa = 3.21GDD490 pKa = 5.16AIRR493 pKa = 11.84ADD495 pKa = 3.43LAAAEE500 pKa = 4.43EE501 pKa = 4.76PVTLTWSDD509 pKa = 3.31QTEE512 pKa = 4.1VSEE515 pKa = 4.25IATGGLVSDD524 pKa = 4.72FSSYY528 pKa = 11.09GLSADD533 pKa = 3.82LTVKK537 pKa = 9.97PQVVAPGGFINSAYY551 pKa = 9.16PLEE554 pKa = 4.9AGPSGYY560 pKa = 8.93ATLSGTSMAAPHH572 pKa = 5.79VAGAAALILEE582 pKa = 4.83VNPALNPSQVLTSLQNTADD601 pKa = 3.86PLLWSGNPDD610 pKa = 3.2LGFPEE615 pKa = 4.48PVHH618 pKa = 6.07RR619 pKa = 11.84QGAGMINIPNAIFANLWGTQVTPSQINLYY648 pKa = 10.96DD649 pKa = 3.83NDD651 pKa = 3.88TGASEE656 pKa = 4.21THH658 pKa = 6.3TLTIHH663 pKa = 6.26NRR665 pKa = 11.84DD666 pKa = 3.44EE667 pKa = 4.56VEE669 pKa = 4.06FTYY672 pKa = 11.1SFVNLAGIATAGINQLPNYY691 pKa = 8.87YY692 pKa = 9.94GAAATTEE699 pKa = 4.66FSAEE703 pKa = 4.25TVTVPAGGTATVDD716 pKa = 3.36VTITEE721 pKa = 4.34PEE723 pKa = 4.25GFAGGIYY730 pKa = 10.16GGQIVVLGEE739 pKa = 4.08DD740 pKa = 3.12ASGYY744 pKa = 4.54TTQSIVSYY752 pKa = 10.67GGLAGDD758 pKa = 4.59YY759 pKa = 7.32EE760 pKa = 4.44TDD762 pKa = 3.52LAFMEE767 pKa = 4.02QWTYY771 pKa = 11.14RR772 pKa = 11.84DD773 pKa = 3.22YY774 pKa = 11.41GYY776 pKa = 9.13TEE778 pKa = 4.29EE779 pKa = 4.81EE780 pKa = 3.97VGEE783 pKa = 4.84LIDD786 pKa = 3.82EE787 pKa = 5.62PIYY790 pKa = 9.68STPGLGVLEE799 pKa = 5.16DD800 pKa = 3.75CTKK803 pKa = 10.83LVDD806 pKa = 4.09GEE808 pKa = 4.64CVSEE812 pKa = 5.13GNYY815 pKa = 10.29SWGHH819 pKa = 5.93DD820 pKa = 3.34EE821 pKa = 4.44YY822 pKa = 11.27VYY824 pKa = 11.52SMEE827 pKa = 6.48DD828 pKa = 3.09GDD830 pKa = 3.8TPYY833 pKa = 11.39AVLHH837 pKa = 6.36IEE839 pKa = 4.25NPVSYY844 pKa = 9.78MKK846 pKa = 10.41IEE848 pKa = 4.28AFHH851 pKa = 6.9ANADD855 pKa = 3.86GTKK858 pKa = 9.77GDD860 pKa = 4.54PVSPDD865 pKa = 3.14YY866 pKa = 11.38NVVYY870 pKa = 10.57EE871 pKa = 4.29SDD873 pKa = 3.87GEE875 pKa = 4.45GAVPGIQVYY884 pKa = 9.22QWDD887 pKa = 3.88GTYY890 pKa = 10.76VKK892 pKa = 10.58SADD895 pKa = 3.34SEE897 pKa = 4.61QYY899 pKa = 11.06LKK901 pKa = 11.06ALDD904 pKa = 3.79GDD906 pKa = 4.31YY907 pKa = 10.83VIEE910 pKa = 4.18VTAVKK915 pKa = 10.67GLGSIDD921 pKa = 3.9NPDD924 pKa = 3.59HH925 pKa = 7.3VEE927 pKa = 3.88VWTSPAFTIDD937 pKa = 3.33RR938 pKa = 11.84DD939 pKa = 4.11GVPGEE944 pKa = 4.32GPGDD948 pKa = 3.64GSIDD952 pKa = 3.54EE953 pKa = 4.53EE954 pKa = 4.24ADD956 pKa = 3.4KK957 pKa = 11.61GFFLYY962 pKa = 11.01NDD964 pKa = 3.92FEE966 pKa = 4.73SAADD970 pKa = 3.51HH971 pKa = 5.94YY972 pKa = 11.47FMYY975 pKa = 10.54GRR977 pKa = 11.84DD978 pKa = 3.52GDD980 pKa = 4.19EE981 pKa = 4.29VFVGDD986 pKa = 4.12WDD988 pKa = 4.13ADD990 pKa = 3.84GVDD993 pKa = 3.94TIGVRR998 pKa = 11.84RR999 pKa = 11.84GHH1001 pKa = 5.62TFYY1004 pKa = 11.25VQNQLIGGNAEE1015 pKa = 3.88VEE1017 pKa = 4.81FKK1019 pKa = 10.73FGRR1022 pKa = 11.84DD1023 pKa = 2.91GDD1025 pKa = 4.34EE1026 pKa = 4.09ILVGDD1031 pKa = 4.83WNADD1035 pKa = 3.32GYY1037 pKa = 10.48DD1038 pKa = 3.39TFAVRR1043 pKa = 11.84RR1044 pKa = 11.84GVTIYY1049 pKa = 10.7AQNEE1053 pKa = 4.58LIGGNAEE1060 pKa = 3.95VEE1062 pKa = 4.56FKK1064 pKa = 10.65YY1065 pKa = 11.07GRR1067 pKa = 11.84VGDD1070 pKa = 4.54DD1071 pKa = 4.11LFAGDD1076 pKa = 4.53WDD1078 pKa = 3.85GDD1080 pKa = 4.09GYY1082 pKa = 10.67DD1083 pKa = 3.35TFAVRR1088 pKa = 11.84RR1089 pKa = 11.84GNSFYY1094 pKa = 11.13VQNSLVGGNAEE1105 pKa = 4.05DD1106 pKa = 3.9TFHH1109 pKa = 7.28FGHH1112 pKa = 6.72EE1113 pKa = 4.34SDD1115 pKa = 3.81TVLVGDD1121 pKa = 4.15WDD1123 pKa = 4.29GDD1125 pKa = 4.01LKK1127 pKa = 11.08DD1128 pKa = 3.74TVALRR1133 pKa = 11.84RR1134 pKa = 11.84GDD1136 pKa = 3.42TFYY1139 pKa = 11.56VSNSLSSGSADD1150 pKa = 3.03RR1151 pKa = 11.84SFVLGRR1157 pKa = 11.84DD1158 pKa = 3.18SDD1160 pKa = 4.07EE1161 pKa = 4.35VLVGDD1166 pKa = 4.31WNGDD1170 pKa = 3.76GIDD1173 pKa = 3.68SVGLRR1178 pKa = 11.84RR1179 pKa = 4.23

Molecular weight: 123.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q9G536|A0A3Q9G536_9ACTO Riboflavin synthase OS=Flaviflexus sp. H23T48 OX=2496867 GN=EJ997_09760 PE=4 SV=1
MM1 pKa = 7.41VKK3 pKa = 9.03RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84KK12 pKa = 8.78RR13 pKa = 11.84AKK15 pKa = 9.18VHH17 pKa = 5.49GFRR20 pKa = 11.84KK21 pKa = 9.95RR22 pKa = 11.84MSTRR26 pKa = 11.84AGRR29 pKa = 11.84AILSGRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.9GRR40 pKa = 11.84AKK42 pKa = 10.68LSAA45 pKa = 3.92

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2334

0

2334

747752

32

2932

320.4

34.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.771 ± 0.054

0.647 ± 0.015

6.36 ± 0.047

6.808 ± 0.05

3.135 ± 0.031

8.593 ± 0.05

1.964 ± 0.022

5.619 ± 0.036

3.143 ± 0.038

9.424 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.241 ± 0.024

2.853 ± 0.026

4.989 ± 0.034

2.976 ± 0.027

6.03 ± 0.053

6.282 ± 0.039

6.26 ± 0.048

8.132 ± 0.047

1.434 ± 0.02

2.34 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski