Amino acid dipepetide frequency for Salmonella phage SSU5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.091AlaAla: 10.091 ± 1.469
1.052AlaCys: 1.052 ± 0.176
5.818AlaAsp: 5.818 ± 0.45
6.344AlaGlu: 6.344 ± 0.438
3.057AlaPhe: 3.057 ± 0.306
7.297AlaGly: 7.297 ± 0.664
1.446AlaHis: 1.446 ± 0.182
4.766AlaIle: 4.766 ± 0.386
6.147AlaLys: 6.147 ± 0.418
7.922AlaLeu: 7.922 ± 0.543
3.188AlaMet: 3.188 ± 0.325
3.747AlaAsn: 3.747 ± 0.383
2.597AlaPro: 2.597 ± 0.365
3.09AlaGln: 3.09 ± 0.435
5.161AlaArg: 5.161 ± 0.456
5.982AlaSer: 5.982 ± 0.932
5.588AlaThr: 5.588 ± 0.659
5.358AlaVal: 5.358 ± 0.387
0.986AlaTrp: 0.986 ± 0.192
2.695AlaTyr: 2.695 ± 0.285
0.0AlaXaa: 0.0 ± 0.0
Cys
0.657CysAla: 0.657 ± 0.124
0.197CysCys: 0.197 ± 0.095
0.986CysAsp: 0.986 ± 0.181
1.019CysGlu: 1.019 ± 0.179
0.46CysPhe: 0.46 ± 0.108
0.855CysGly: 0.855 ± 0.165
0.329CysHis: 0.329 ± 0.095
0.625CysIle: 0.625 ± 0.152
0.592CysLys: 0.592 ± 0.144
0.887CysLeu: 0.887 ± 0.166
0.329CysMet: 0.329 ± 0.097
0.329CysAsn: 0.329 ± 0.092
0.559CysPro: 0.559 ± 0.12
0.657CysGln: 0.657 ± 0.165
0.69CysArg: 0.69 ± 0.187
0.427CysSer: 0.427 ± 0.108
0.329CysThr: 0.329 ± 0.086
0.526CysVal: 0.526 ± 0.147
0.131CysTrp: 0.131 ± 0.069
0.197CysTyr: 0.197 ± 0.09
0.0CysXaa: 0.0 ± 0.0
Asp
5.456AspAla: 5.456 ± 0.329
0.592AspCys: 0.592 ± 0.134
4.963AspAsp: 4.963 ± 0.502
4.569AspGlu: 4.569 ± 0.473
2.432AspPhe: 2.432 ± 0.27
4.668AspGly: 4.668 ± 0.406
0.789AspHis: 0.789 ± 0.162
3.386AspIle: 3.386 ± 0.356
3.912AspLys: 3.912 ± 0.292
5.325AspLeu: 5.325 ± 0.443
1.939AspMet: 1.939 ± 0.217
2.334AspAsn: 2.334 ± 0.259
2.465AspPro: 2.465 ± 0.268
2.005AspGln: 2.005 ± 0.196
2.728AspArg: 2.728 ± 0.308
3.879AspSer: 3.879 ± 0.392
3.09AspThr: 3.09 ± 0.311
3.912AspVal: 3.912 ± 0.375
0.723AspTrp: 0.723 ± 0.172
1.939AspTyr: 1.939 ± 0.226
0.0AspXaa: 0.0 ± 0.0
Glu
7.034GluAla: 7.034 ± 0.529
0.559GluCys: 0.559 ± 0.155
4.24GluAsp: 4.24 ± 0.466
5.686GluGlu: 5.686 ± 0.507
2.432GluPhe: 2.432 ± 0.341
4.273GluGly: 4.273 ± 0.351
1.249GluHis: 1.249 ± 0.207
4.7GluIle: 4.7 ± 0.354
4.832GluLys: 4.832 ± 0.442
7.231GluLeu: 7.231 ± 0.498
2.761GluMet: 2.761 ± 0.293
2.465GluAsn: 2.465 ± 0.297
2.104GluPro: 2.104 ± 0.298
3.024GluGln: 3.024 ± 0.289
5.193GluArg: 5.193 ± 0.523
4.766GluSer: 4.766 ± 0.442
4.076GluThr: 4.076 ± 0.317
4.799GluVal: 4.799 ± 0.439
0.756GluTrp: 0.756 ± 0.172
1.939GluTyr: 1.939 ± 0.18
0.0GluXaa: 0.0 ± 0.0
Phe
3.09PheAla: 3.09 ± 0.359
0.625PheCys: 0.625 ± 0.144
3.057PheAsp: 3.057 ± 0.316
2.4PheGlu: 2.4 ± 0.277
1.315PhePhe: 1.315 ± 0.235
2.728PheGly: 2.728 ± 0.269
0.756PheHis: 0.756 ± 0.151
2.531PheIle: 2.531 ± 0.236
2.301PheLys: 2.301 ± 0.283
2.4PheLeu: 2.4 ± 0.266
0.953PheMet: 0.953 ± 0.169
1.874PheAsn: 1.874 ± 0.253
1.611PhePro: 1.611 ± 0.241
1.183PheGln: 1.183 ± 0.206
1.512PheArg: 1.512 ± 0.192
2.4PheSer: 2.4 ± 0.305
2.564PheThr: 2.564 ± 0.274
2.268PheVal: 2.268 ± 0.3
0.427PheTrp: 0.427 ± 0.102
1.315PheTyr: 1.315 ± 0.219
0.0PheXaa: 0.0 ± 0.0
Gly
5.555GlyAla: 5.555 ± 0.472
0.789GlyCys: 0.789 ± 0.155
4.076GlyAsp: 4.076 ± 0.376
5.424GlyGlu: 5.424 ± 0.431
3.057GlyPhe: 3.057 ± 0.323
4.306GlyGly: 4.306 ± 0.556
1.282GlyHis: 1.282 ± 0.236
4.109GlyIle: 4.109 ± 0.349
4.898GlyLys: 4.898 ± 0.401
5.686GlyLeu: 5.686 ± 0.446
1.906GlyMet: 1.906 ± 0.194
2.794GlyAsn: 2.794 ± 0.427
1.085GlyPro: 1.085 ± 0.175
2.4GlyGln: 2.4 ± 0.342
3.747GlyArg: 3.747 ± 0.353
5.424GlySer: 5.424 ± 0.45
3.386GlyThr: 3.386 ± 0.413
6.048GlyVal: 6.048 ± 0.536
0.887GlyTrp: 0.887 ± 0.171
2.367GlyTyr: 2.367 ± 0.275
0.0GlyXaa: 0.0 ± 0.0
His
1.348HisAla: 1.348 ± 0.221
0.197HisCys: 0.197 ± 0.073
0.986HisAsp: 0.986 ± 0.226
1.348HisGlu: 1.348 ± 0.212
1.019HisPhe: 1.019 ± 0.211
0.953HisGly: 0.953 ± 0.186
0.296HisHis: 0.296 ± 0.119
1.282HisIle: 1.282 ± 0.246
1.183HisLys: 1.183 ± 0.189
1.906HisLeu: 1.906 ± 0.243
0.526HisMet: 0.526 ± 0.109
0.822HisAsn: 0.822 ± 0.143
0.657HisPro: 0.657 ± 0.151
0.69HisGln: 0.69 ± 0.142
1.019HisArg: 1.019 ± 0.198
1.085HisSer: 1.085 ± 0.185
1.052HisThr: 1.052 ± 0.228
1.019HisVal: 1.019 ± 0.178
0.197HisTrp: 0.197 ± 0.076
0.756HisTyr: 0.756 ± 0.155
0.0HisXaa: 0.0 ± 0.0
Ile
4.996IleAla: 4.996 ± 0.399
0.855IleCys: 0.855 ± 0.166
4.372IleAsp: 4.372 ± 0.379
4.339IleGlu: 4.339 ± 0.34
1.643IlePhe: 1.643 ± 0.241
3.977IleGly: 3.977 ± 0.285
0.789IleHis: 0.789 ± 0.191
3.123IleIle: 3.123 ± 0.36
3.254IleLys: 3.254 ± 0.347
3.517IleLeu: 3.517 ± 0.38
1.15IleMet: 1.15 ± 0.174
2.925IleAsn: 2.925 ± 0.359
2.498IlePro: 2.498 ± 0.272
2.071IleGln: 2.071 ± 0.201
3.418IleArg: 3.418 ± 0.285
3.813IleSer: 3.813 ± 0.301
3.879IleThr: 3.879 ± 0.458
4.043IleVal: 4.043 ± 0.39
0.592IleTrp: 0.592 ± 0.134
1.742IleTyr: 1.742 ± 0.23
0.0IleXaa: 0.0 ± 0.0
Lys
6.837LysAla: 6.837 ± 0.56
0.789LysCys: 0.789 ± 0.159
2.728LysAsp: 2.728 ± 0.289
5.325LysGlu: 5.325 ± 0.512
1.611LysPhe: 1.611 ± 0.19
3.714LysGly: 3.714 ± 0.365
1.446LysHis: 1.446 ± 0.239
3.649LysIle: 3.649 ± 0.273
3.517LysLys: 3.517 ± 0.376
5.555LysLeu: 5.555 ± 0.447
1.874LysMet: 1.874 ± 0.203
2.597LysAsn: 2.597 ± 0.316
2.761LysPro: 2.761 ± 0.31
2.005LysGln: 2.005 ± 0.268
4.569LysArg: 4.569 ± 0.396
4.076LysSer: 4.076 ± 0.356
3.879LysThr: 3.879 ± 0.32
3.846LysVal: 3.846 ± 0.369
0.822LysTrp: 0.822 ± 0.168
1.742LysTyr: 1.742 ± 0.251
0.0LysXaa: 0.0 ± 0.0
Leu
7.724LeuAla: 7.724 ± 0.489
0.986LeuCys: 0.986 ± 0.195
5.522LeuAsp: 5.522 ± 0.478
6.41LeuGlu: 6.41 ± 0.467
2.695LeuPhe: 2.695 ± 0.326
5.128LeuGly: 5.128 ± 0.343
1.118LeuHis: 1.118 ± 0.21
4.963LeuIle: 4.963 ± 0.418
5.818LeuLys: 5.818 ± 0.456
6.574LeuLeu: 6.574 ± 0.54
2.991LeuMet: 2.991 ± 0.302
3.681LeuAsn: 3.681 ± 0.353
3.879LeuPro: 3.879 ± 0.358
2.63LeuGln: 2.63 ± 0.335
5.029LeuArg: 5.029 ± 0.464
5.588LeuSer: 5.588 ± 0.399
4.372LeuThr: 4.372 ± 0.438
6.147LeuVal: 6.147 ± 0.412
1.052LeuTrp: 1.052 ± 0.218
1.939LeuTyr: 1.939 ± 0.225
0.0LeuXaa: 0.0 ± 0.0
Met
2.301MetAla: 2.301 ± 0.293
0.329MetCys: 0.329 ± 0.103
1.216MetAsp: 1.216 ± 0.203
1.906MetGlu: 1.906 ± 0.219
0.887MetPhe: 0.887 ± 0.197
1.775MetGly: 1.775 ± 0.269
0.394MetHis: 0.394 ± 0.109
1.841MetIle: 1.841 ± 0.242
2.564MetLys: 2.564 ± 0.221
2.531MetLeu: 2.531 ± 0.311
1.183MetMet: 1.183 ± 0.21
1.446MetAsn: 1.446 ± 0.2
1.085MetPro: 1.085 ± 0.192
0.953MetGln: 0.953 ± 0.18
1.643MetArg: 1.643 ± 0.232
2.958MetSer: 2.958 ± 0.285
2.202MetThr: 2.202 ± 0.239
2.202MetVal: 2.202 ± 0.291
0.362MetTrp: 0.362 ± 0.112
0.625MetTyr: 0.625 ± 0.142
0.0MetXaa: 0.0 ± 0.0
Asn
4.142AsnAla: 4.142 ± 0.483
0.23AsnCys: 0.23 ± 0.078
2.432AsnAsp: 2.432 ± 0.236
3.123AsnGlu: 3.123 ± 0.299
1.545AsnPhe: 1.545 ± 0.244
3.714AsnGly: 3.714 ± 0.437
0.657AsnHis: 0.657 ± 0.173
2.4AsnIle: 2.4 ± 0.285
2.498AsnLys: 2.498 ± 0.233
3.057AsnLeu: 3.057 ± 0.294
1.052AsnMet: 1.052 ± 0.153
1.578AsnAsn: 1.578 ± 0.269
1.939AsnPro: 1.939 ± 0.258
1.446AsnGln: 1.446 ± 0.236
1.808AsnArg: 1.808 ± 0.287
2.794AsnSer: 2.794 ± 0.36
2.564AsnThr: 2.564 ± 0.308
2.991AsnVal: 2.991 ± 0.346
0.69AsnTrp: 0.69 ± 0.142
1.118AsnTyr: 1.118 ± 0.194
0.0AsnXaa: 0.0 ± 0.0
Pro
2.695ProAla: 2.695 ± 0.372
0.362ProCys: 0.362 ± 0.089
2.465ProAsp: 2.465 ± 0.25
3.714ProGlu: 3.714 ± 0.47
1.808ProPhe: 1.808 ± 0.232
2.268ProGly: 2.268 ± 0.239
0.625ProHis: 0.625 ± 0.153
1.676ProIle: 1.676 ± 0.207
2.268ProLys: 2.268 ± 0.276
2.695ProLeu: 2.695 ± 0.314
1.085ProMet: 1.085 ± 0.2
1.643ProAsn: 1.643 ± 0.181
1.052ProPro: 1.052 ± 0.192
1.085ProGln: 1.085 ± 0.183
1.413ProArg: 1.413 ± 0.209
2.104ProSer: 2.104 ± 0.299
2.104ProThr: 2.104 ± 0.278
3.221ProVal: 3.221 ± 0.337
0.46ProTrp: 0.46 ± 0.129
1.249ProTyr: 1.249 ± 0.221
0.0ProXaa: 0.0 ± 0.0
Gln
2.958GlnAla: 2.958 ± 0.343
0.131GlnCys: 0.131 ± 0.064
1.282GlnAsp: 1.282 ± 0.205
2.367GlnGlu: 2.367 ± 0.254
1.643GlnPhe: 1.643 ± 0.238
1.775GlnGly: 1.775 ± 0.213
0.855GlnHis: 0.855 ± 0.167
2.235GlnIle: 2.235 ± 0.324
2.498GlnLys: 2.498 ± 0.367
3.287GlnLeu: 3.287 ± 0.366
1.512GlnMet: 1.512 ± 0.26
1.709GlnAsn: 1.709 ± 0.232
1.019GlnPro: 1.019 ± 0.174
1.183GlnGln: 1.183 ± 0.193
1.972GlnArg: 1.972 ± 0.28
2.137GlnSer: 2.137 ± 0.251
1.742GlnThr: 1.742 ± 0.23
2.104GlnVal: 2.104 ± 0.264
0.559GlnTrp: 0.559 ± 0.116
1.052GlnTyr: 1.052 ± 0.23
0.0GlnXaa: 0.0 ± 0.0
Arg
5.555ArgAla: 5.555 ± 0.397
0.329ArgCys: 0.329 ± 0.096
3.55ArgAsp: 3.55 ± 0.329
4.536ArgGlu: 4.536 ± 0.389
2.235ArgPhe: 2.235 ± 0.261
3.977ArgGly: 3.977 ± 0.325
1.676ArgHis: 1.676 ± 0.275
3.123ArgIle: 3.123 ± 0.304
3.386ArgLys: 3.386 ± 0.338
5.489ArgLeu: 5.489 ± 0.47
1.381ArgMet: 1.381 ± 0.248
2.301ArgAsn: 2.301 ± 0.231
1.906ArgPro: 1.906 ± 0.252
2.268ArgGln: 2.268 ± 0.285
2.991ArgArg: 2.991 ± 0.335
2.597ArgSer: 2.597 ± 0.298
2.925ArgThr: 2.925 ± 0.3
3.977ArgVal: 3.977 ± 0.372
0.592ArgTrp: 0.592 ± 0.126
1.643ArgTyr: 1.643 ± 0.228
0.0ArgXaa: 0.0 ± 0.0
Ser
6.41SerAla: 6.41 ± 1.177
0.657SerCys: 0.657 ± 0.147
3.353SerAsp: 3.353 ± 0.308
4.109SerGlu: 4.109 ± 0.407
2.761SerPhe: 2.761 ± 0.252
5.917SerGly: 5.917 ± 0.473
1.019SerHis: 1.019 ± 0.211
3.912SerIle: 3.912 ± 0.384
3.616SerLys: 3.616 ± 0.329
6.442SerLeu: 6.442 ± 0.573
1.611SerMet: 1.611 ± 0.196
2.597SerAsn: 2.597 ± 0.245
2.334SerPro: 2.334 ± 0.279
1.972SerGln: 1.972 ± 0.261
2.958SerArg: 2.958 ± 0.252
3.846SerSer: 3.846 ± 0.436
3.616SerThr: 3.616 ± 0.379
5.325SerVal: 5.325 ± 0.457
0.657SerTrp: 0.657 ± 0.126
1.676SerTyr: 1.676 ± 0.233
0.0SerXaa: 0.0 ± 0.0
Thr
5.917ThrAla: 5.917 ± 0.628
0.493ThrCys: 0.493 ± 0.112
2.728ThrAsp: 2.728 ± 0.333
3.254ThrGlu: 3.254 ± 0.349
2.4ThrPhe: 2.4 ± 0.258
4.799ThrGly: 4.799 ± 0.384
1.249ThrHis: 1.249 ± 0.228
3.156ThrIle: 3.156 ± 0.314
3.353ThrLys: 3.353 ± 0.295
4.832ThrLeu: 4.832 ± 0.331
1.512ThrMet: 1.512 ± 0.183
2.728ThrAsn: 2.728 ± 0.385
2.662ThrPro: 2.662 ± 0.34
1.512ThrGln: 1.512 ± 0.223
2.662ThrArg: 2.662 ± 0.321
3.616ThrSer: 3.616 ± 0.5
3.353ThrThr: 3.353 ± 0.418
4.7ThrVal: 4.7 ± 0.451
0.756ThrTrp: 0.756 ± 0.196
1.545ThrTyr: 1.545 ± 0.23
0.0ThrXaa: 0.0 ± 0.0
Val
6.804ValAla: 6.804 ± 0.424
0.986ValCys: 0.986 ± 0.174
4.569ValAsp: 4.569 ± 0.371
5.884ValGlu: 5.884 ± 0.469
2.564ValPhe: 2.564 ± 0.278
4.405ValGly: 4.405 ± 0.46
1.315ValHis: 1.315 ± 0.234
3.616ValIle: 3.616 ± 0.307
4.635ValLys: 4.635 ± 0.407
5.128ValLeu: 5.128 ± 0.433
2.235ValMet: 2.235 ± 0.297
2.893ValAsn: 2.893 ± 0.32
2.137ValPro: 2.137 ± 0.262
2.071ValGln: 2.071 ± 0.244
4.47ValArg: 4.47 ± 0.461
5.029ValSer: 5.029 ± 0.451
4.174ValThr: 4.174 ± 0.545
4.635ValVal: 4.635 ± 0.392
0.625ValTrp: 0.625 ± 0.144
2.432ValTyr: 2.432 ± 0.34
0.0ValXaa: 0.0 ± 0.0
Trp
0.855TrpAla: 0.855 ± 0.173
0.23TrpCys: 0.23 ± 0.087
0.756TrpAsp: 0.756 ± 0.134
0.329TrpGlu: 0.329 ± 0.112
0.46TrpPhe: 0.46 ± 0.117
0.559TrpGly: 0.559 ± 0.122
0.46TrpHis: 0.46 ± 0.113
0.559TrpIle: 0.559 ± 0.118
0.822TrpLys: 0.822 ± 0.18
0.953TrpLeu: 0.953 ± 0.154
0.592TrpMet: 0.592 ± 0.145
0.394TrpAsn: 0.394 ± 0.1
0.296TrpPro: 0.296 ± 0.12
0.394TrpGln: 0.394 ± 0.106
0.986TrpArg: 0.986 ± 0.173
0.855TrpSer: 0.855 ± 0.183
0.427TrpThr: 0.427 ± 0.126
1.15TrpVal: 1.15 ± 0.207
0.066TrpTrp: 0.066 ± 0.044
0.526TrpTyr: 0.526 ± 0.12
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.939TyrAla: 1.939 ± 0.228
0.394TyrCys: 0.394 ± 0.111
2.005TyrAsp: 2.005 ± 0.248
2.038TyrGlu: 2.038 ± 0.28
1.282TyrPhe: 1.282 ± 0.208
2.137TyrGly: 2.137 ± 0.277
0.592TyrHis: 0.592 ± 0.137
1.348TyrIle: 1.348 ± 0.229
1.249TyrLys: 1.249 ± 0.231
2.827TyrLeu: 2.827 ± 0.319
0.592TyrMet: 0.592 ± 0.152
0.92TyrAsn: 0.92 ± 0.16
1.348TyrPro: 1.348 ± 0.208
1.381TyrGln: 1.381 ± 0.227
2.465TyrArg: 2.465 ± 0.328
1.413TyrSer: 1.413 ± 0.251
1.906TyrThr: 1.906 ± 0.217
2.334TyrVal: 2.334 ± 0.313
0.329TyrTrp: 0.329 ± 0.102
0.789TyrTyr: 0.789 ± 0.169
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 130 proteins (30424 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski