Amino acid dipepetide frequency for Arthrobacter phage Richie

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.773AlaAla: 16.773 ± 1.601
1.004AlaCys: 1.004 ± 0.264
8.268AlaAsp: 8.268 ± 0.831
8.209AlaGlu: 8.209 ± 0.789
2.658AlaPhe: 2.658 ± 0.341
11.044AlaGly: 11.044 ± 1.202
1.831AlaHis: 1.831 ± 0.35
4.784AlaIle: 4.784 ± 0.556
5.965AlaLys: 5.965 ± 0.683
9.154AlaLeu: 9.154 ± 0.821
3.307AlaMet: 3.307 ± 0.501
4.075AlaAsn: 4.075 ± 0.518
4.311AlaPro: 4.311 ± 0.58
5.374AlaGln: 5.374 ± 0.622
7.501AlaArg: 7.501 ± 0.68
5.256AlaSer: 5.256 ± 0.78
6.556AlaThr: 6.556 ± 0.532
7.973AlaVal: 7.973 ± 0.665
2.303AlaTrp: 2.303 ± 0.366
2.54AlaTyr: 2.54 ± 0.37
0.0AlaXaa: 0.0 ± 0.0
Cys
0.709CysAla: 0.709 ± 0.246
0.236CysCys: 0.236 ± 0.111
0.768CysAsp: 0.768 ± 0.225
0.709CysGlu: 0.709 ± 0.21
0.118CysPhe: 0.118 ± 0.131
1.24CysGly: 1.24 ± 0.369
0.413CysHis: 0.413 ± 0.162
0.118CysIle: 0.118 ± 0.096
0.945CysLys: 0.945 ± 0.265
0.532CysLeu: 0.532 ± 0.186
0.118CysMet: 0.118 ± 0.103
0.236CysAsn: 0.236 ± 0.11
1.417CysPro: 1.417 ± 0.318
0.118CysGln: 0.118 ± 0.092
0.413CysArg: 0.413 ± 0.221
0.65CysSer: 0.65 ± 0.212
1.063CysThr: 1.063 ± 0.276
0.532CysVal: 0.532 ± 0.167
0.177CysTrp: 0.177 ± 0.105
0.177CysTyr: 0.177 ± 0.104
0.0CysXaa: 0.0 ± 0.0
Asp
7.264AspAla: 7.264 ± 0.669
0.532AspCys: 0.532 ± 0.16
3.721AspAsp: 3.721 ± 0.578
3.957AspGlu: 3.957 ± 0.482
2.008AspPhe: 2.008 ± 0.325
6.556AspGly: 6.556 ± 0.791
1.063AspHis: 1.063 ± 0.28
2.953AspIle: 2.953 ± 0.395
2.54AspLys: 2.54 ± 0.393
5.374AspLeu: 5.374 ± 0.548
1.476AspMet: 1.476 ± 0.326
1.299AspAsn: 1.299 ± 0.292
4.193AspPro: 4.193 ± 0.679
1.772AspGln: 1.772 ± 0.318
3.898AspArg: 3.898 ± 0.424
2.658AspSer: 2.658 ± 0.394
2.894AspThr: 2.894 ± 0.474
5.079AspVal: 5.079 ± 0.586
1.24AspTrp: 1.24 ± 0.244
1.654AspTyr: 1.654 ± 0.346
0.0AspXaa: 0.0 ± 0.0
Glu
8.091GluAla: 8.091 ± 0.802
0.827GluCys: 0.827 ± 0.276
3.012GluAsp: 3.012 ± 0.453
2.894GluGlu: 2.894 ± 0.404
1.595GluPhe: 1.595 ± 0.238
4.666GluGly: 4.666 ± 0.558
1.536GluHis: 1.536 ± 0.311
2.658GluIle: 2.658 ± 0.419
3.071GluLys: 3.071 ± 0.488
4.311GluLeu: 4.311 ± 0.5
1.536GluMet: 1.536 ± 0.331
2.244GluAsn: 2.244 ± 0.301
3.957GluPro: 3.957 ± 0.605
2.953GluGln: 2.953 ± 0.474
4.489GluArg: 4.489 ± 0.509
3.425GluSer: 3.425 ± 0.479
3.78GluThr: 3.78 ± 0.457
4.193GluVal: 4.193 ± 0.491
1.949GluTrp: 1.949 ± 0.35
1.949GluTyr: 1.949 ± 0.367
0.0GluXaa: 0.0 ± 0.0
Phe
2.953PheAla: 2.953 ± 0.429
0.532PheCys: 0.532 ± 0.213
2.362PheAsp: 2.362 ± 0.352
1.476PheGlu: 1.476 ± 0.338
0.945PhePhe: 0.945 ± 0.242
2.717PheGly: 2.717 ± 0.395
1.181PheHis: 1.181 ± 0.291
0.886PheIle: 0.886 ± 0.25
1.181PheLys: 1.181 ± 0.315
1.595PheLeu: 1.595 ± 0.378
0.768PheMet: 0.768 ± 0.262
1.004PheAsn: 1.004 ± 0.254
1.122PhePro: 1.122 ± 0.288
0.886PheGln: 0.886 ± 0.188
1.476PheArg: 1.476 ± 0.272
1.654PheSer: 1.654 ± 0.266
2.481PheThr: 2.481 ± 0.39
1.654PheVal: 1.654 ± 0.32
0.591PheTrp: 0.591 ± 0.174
0.827PheTyr: 0.827 ± 0.239
0.0PheXaa: 0.0 ± 0.0
Gly
8.386GlyAla: 8.386 ± 1.221
0.827GlyCys: 0.827 ± 0.235
4.489GlyAsp: 4.489 ± 0.437
4.607GlyGlu: 4.607 ± 0.462
2.776GlyPhe: 2.776 ± 0.364
7.146GlyGly: 7.146 ± 0.746
1.595GlyHis: 1.595 ± 0.351
3.248GlyIle: 3.248 ± 0.482
4.193GlyLys: 4.193 ± 0.499
6.201GlyLeu: 6.201 ± 0.86
2.008GlyMet: 2.008 ± 0.367
3.485GlyAsn: 3.485 ± 0.464
3.485GlyPro: 3.485 ± 0.604
2.717GlyGln: 2.717 ± 0.534
5.138GlyArg: 5.138 ± 0.543
4.489GlySer: 4.489 ± 0.546
5.552GlyThr: 5.552 ± 0.727
5.433GlyVal: 5.433 ± 0.565
2.067GlyTrp: 2.067 ± 0.38
2.717GlyTyr: 2.717 ± 0.367
0.0GlyXaa: 0.0 ± 0.0
His
2.362HisAla: 2.362 ± 0.42
0.472HisCys: 0.472 ± 0.168
1.122HisAsp: 1.122 ± 0.242
1.89HisGlu: 1.89 ± 0.415
0.709HisPhe: 0.709 ± 0.214
1.358HisGly: 1.358 ± 0.342
0.886HisHis: 0.886 ± 0.294
1.299HisIle: 1.299 ± 0.26
0.827HisLys: 0.827 ± 0.23
1.536HisLeu: 1.536 ± 0.304
0.65HisMet: 0.65 ± 0.177
0.945HisAsn: 0.945 ± 0.277
1.063HisPro: 1.063 ± 0.26
0.65HisGln: 0.65 ± 0.178
1.181HisArg: 1.181 ± 0.278
1.004HisSer: 1.004 ± 0.308
1.358HisThr: 1.358 ± 0.298
1.654HisVal: 1.654 ± 0.281
0.354HisTrp: 0.354 ± 0.153
0.768HisTyr: 0.768 ± 0.244
0.0HisXaa: 0.0 ± 0.0
Ile
5.138IleAla: 5.138 ± 0.625
0.236IleCys: 0.236 ± 0.12
2.835IleAsp: 2.835 ± 0.444
3.189IleGlu: 3.189 ± 0.468
1.358IlePhe: 1.358 ± 0.268
3.071IleGly: 3.071 ± 0.407
0.827IleHis: 0.827 ± 0.229
1.831IleIle: 1.831 ± 0.402
1.949IleLys: 1.949 ± 0.327
2.894IleLeu: 2.894 ± 0.405
0.65IleMet: 0.65 ± 0.189
1.24IleAsn: 1.24 ± 0.262
2.599IlePro: 2.599 ± 0.387
1.654IleGln: 1.654 ± 0.3
3.425IleArg: 3.425 ± 0.486
2.717IleSer: 2.717 ± 0.428
4.252IleThr: 4.252 ± 0.502
3.248IleVal: 3.248 ± 0.433
0.118IleTrp: 0.118 ± 0.085
0.827IleTyr: 0.827 ± 0.226
0.0IleXaa: 0.0 ± 0.0
Lys
6.142LysAla: 6.142 ± 0.972
0.472LysCys: 0.472 ± 0.215
2.658LysAsp: 2.658 ± 0.447
2.185LysGlu: 2.185 ± 0.454
1.595LysPhe: 1.595 ± 0.274
2.717LysGly: 2.717 ± 0.379
1.063LysHis: 1.063 ± 0.276
1.89LysIle: 1.89 ± 0.332
1.772LysLys: 1.772 ± 0.306
2.599LysLeu: 2.599 ± 0.377
1.181LysMet: 1.181 ± 0.205
1.713LysAsn: 1.713 ± 0.361
3.78LysPro: 3.78 ± 0.549
1.831LysGln: 1.831 ± 0.338
3.189LysArg: 3.189 ± 0.454
2.421LysSer: 2.421 ± 0.352
3.071LysThr: 3.071 ± 0.336
3.071LysVal: 3.071 ± 0.428
0.472LysTrp: 0.472 ± 0.154
1.181LysTyr: 1.181 ± 0.278
0.0LysXaa: 0.0 ± 0.0
Leu
10.217LeuAla: 10.217 ± 1.112
0.709LeuCys: 0.709 ± 0.226
4.607LeuAsp: 4.607 ± 0.604
4.252LeuGlu: 4.252 ± 0.467
1.595LeuPhe: 1.595 ± 0.47
5.729LeuGly: 5.729 ± 0.607
1.536LeuHis: 1.536 ± 0.322
4.252LeuIle: 4.252 ± 0.502
4.016LeuLys: 4.016 ± 0.564
5.729LeuLeu: 5.729 ± 0.538
1.122LeuMet: 1.122 ± 0.252
2.481LeuAsn: 2.481 ± 0.485
5.02LeuPro: 5.02 ± 0.71
2.244LeuGln: 2.244 ± 0.37
4.725LeuArg: 4.725 ± 0.539
4.134LeuSer: 4.134 ± 0.553
6.438LeuThr: 6.438 ± 0.796
3.898LeuVal: 3.898 ± 0.496
1.063LeuTrp: 1.063 ± 0.245
1.831LeuTyr: 1.831 ± 0.303
0.0LeuXaa: 0.0 ± 0.0
Met
2.953MetAla: 2.953 ± 0.469
0.059MetCys: 0.059 ± 0.064
1.24MetAsp: 1.24 ± 0.251
1.004MetGlu: 1.004 ± 0.199
0.827MetPhe: 0.827 ± 0.201
1.536MetGly: 1.536 ± 0.453
0.591MetHis: 0.591 ± 0.187
0.768MetIle: 0.768 ± 0.201
0.827MetLys: 0.827 ± 0.191
1.89MetLeu: 1.89 ± 0.293
0.236MetMet: 0.236 ± 0.135
0.827MetAsn: 0.827 ± 0.195
1.713MetPro: 1.713 ± 0.341
0.591MetGln: 0.591 ± 0.159
1.122MetArg: 1.122 ± 0.29
2.362MetSer: 2.362 ± 0.312
2.717MetThr: 2.717 ± 0.442
1.536MetVal: 1.536 ± 0.272
0.295MetTrp: 0.295 ± 0.151
0.413MetTyr: 0.413 ± 0.135
0.0MetXaa: 0.0 ± 0.0
Asn
4.252AsnAla: 4.252 ± 0.58
0.177AsnCys: 0.177 ± 0.108
1.89AsnAsp: 1.89 ± 0.391
1.831AsnGlu: 1.831 ± 0.409
0.709AsnPhe: 0.709 ± 0.229
3.071AsnGly: 3.071 ± 0.583
1.299AsnHis: 1.299 ± 0.315
1.181AsnIle: 1.181 ± 0.275
1.24AsnLys: 1.24 ± 0.199
2.658AsnLeu: 2.658 ± 0.381
0.65AsnMet: 0.65 ± 0.208
0.827AsnAsn: 0.827 ± 0.237
2.658AsnPro: 2.658 ± 0.487
1.417AsnGln: 1.417 ± 0.268
2.303AsnArg: 2.303 ± 0.392
1.772AsnSer: 1.772 ± 0.352
2.067AsnThr: 2.067 ± 0.369
2.185AsnVal: 2.185 ± 0.362
0.472AsnTrp: 0.472 ± 0.187
0.709AsnTyr: 0.709 ± 0.211
0.0AsnXaa: 0.0 ± 0.0
Pro
6.26ProAla: 6.26 ± 0.579
0.827ProCys: 0.827 ± 0.236
3.957ProAsp: 3.957 ± 0.545
4.725ProGlu: 4.725 ± 0.748
2.008ProPhe: 2.008 ± 0.282
4.784ProGly: 4.784 ± 0.554
0.945ProHis: 0.945 ± 0.219
1.949ProIle: 1.949 ± 0.361
2.185ProLys: 2.185 ± 0.369
4.311ProLeu: 4.311 ± 0.629
1.063ProMet: 1.063 ± 0.264
1.654ProAsn: 1.654 ± 0.349
3.307ProPro: 3.307 ± 0.585
1.595ProGln: 1.595 ± 0.358
3.839ProArg: 3.839 ± 0.655
3.012ProSer: 3.012 ± 0.457
3.13ProThr: 3.13 ± 0.433
4.311ProVal: 4.311 ± 0.592
0.945ProTrp: 0.945 ± 0.201
1.122ProTyr: 1.122 ± 0.252
0.0ProXaa: 0.0 ± 0.0
Gln
4.37GlnAla: 4.37 ± 0.635
0.591GlnCys: 0.591 ± 0.221
2.54GlnAsp: 2.54 ± 0.463
1.772GlnGlu: 1.772 ± 0.382
1.004GlnPhe: 1.004 ± 0.201
3.071GlnGly: 3.071 ± 0.631
0.709GlnHis: 0.709 ± 0.164
1.772GlnIle: 1.772 ± 0.269
1.417GlnLys: 1.417 ± 0.295
2.599GlnLeu: 2.599 ± 0.398
1.004GlnMet: 1.004 ± 0.21
0.827GlnAsn: 0.827 ± 0.211
2.244GlnPro: 2.244 ± 0.389
1.654GlnGln: 1.654 ± 0.285
2.185GlnArg: 2.185 ± 0.325
2.067GlnSer: 2.067 ± 0.342
2.481GlnThr: 2.481 ± 0.461
2.244GlnVal: 2.244 ± 0.357
0.65GlnTrp: 0.65 ± 0.226
0.827GlnTyr: 0.827 ± 0.229
0.0GlnXaa: 0.0 ± 0.0
Arg
6.083ArgAla: 6.083 ± 0.65
1.063ArgCys: 1.063 ± 0.252
3.603ArgAsp: 3.603 ± 0.497
4.252ArgGlu: 4.252 ± 0.419
1.89ArgPhe: 1.89 ± 0.353
4.607ArgGly: 4.607 ± 0.546
1.772ArgHis: 1.772 ± 0.489
4.134ArgIle: 4.134 ± 0.519
2.599ArgLys: 2.599 ± 0.306
5.67ArgLeu: 5.67 ± 0.581
1.831ArgMet: 1.831 ± 0.325
2.244ArgAsn: 2.244 ± 0.357
2.835ArgPro: 2.835 ± 0.461
1.949ArgGln: 1.949 ± 0.411
5.433ArgArg: 5.433 ± 0.731
3.13ArgSer: 3.13 ± 0.355
3.898ArgThr: 3.898 ± 0.433
4.311ArgVal: 4.311 ± 0.598
2.185ArgTrp: 2.185 ± 0.401
2.185ArgTyr: 2.185 ± 0.393
0.0ArgXaa: 0.0 ± 0.0
Ser
5.611SerAla: 5.611 ± 0.505
0.295SerCys: 0.295 ± 0.115
3.425SerAsp: 3.425 ± 0.411
3.839SerGlu: 3.839 ± 0.422
1.24SerPhe: 1.24 ± 0.253
5.493SerGly: 5.493 ± 0.739
0.768SerHis: 0.768 ± 0.24
2.835SerIle: 2.835 ± 0.42
2.54SerLys: 2.54 ± 0.393
4.489SerLeu: 4.489 ± 0.562
1.536SerMet: 1.536 ± 0.282
1.122SerAsn: 1.122 ± 0.26
2.54SerPro: 2.54 ± 0.41
1.949SerGln: 1.949 ± 0.352
3.13SerArg: 3.13 ± 0.395
2.126SerSer: 2.126 ± 0.462
3.544SerThr: 3.544 ± 0.396
3.721SerVal: 3.721 ± 0.536
0.886SerTrp: 0.886 ± 0.263
1.89SerTyr: 1.89 ± 0.294
0.0SerXaa: 0.0 ± 0.0
Thr
8.327ThrAla: 8.327 ± 0.697
0.65ThrCys: 0.65 ± 0.226
4.193ThrAsp: 4.193 ± 0.428
4.666ThrGlu: 4.666 ± 0.664
2.067ThrPhe: 2.067 ± 0.305
4.961ThrGly: 4.961 ± 0.586
1.476ThrHis: 1.476 ± 0.283
3.012ThrIle: 3.012 ± 0.438
2.894ThrLys: 2.894 ± 0.416
4.725ThrLeu: 4.725 ± 0.55
1.417ThrMet: 1.417 ± 0.264
2.481ThrAsn: 2.481 ± 0.36
3.603ThrPro: 3.603 ± 0.474
2.362ThrGln: 2.362 ± 0.401
3.78ThrArg: 3.78 ± 0.473
3.13ThrSer: 3.13 ± 0.443
4.784ThrThr: 4.784 ± 0.595
5.847ThrVal: 5.847 ± 0.678
0.827ThrTrp: 0.827 ± 0.234
2.894ThrTyr: 2.894 ± 0.455
0.0ThrXaa: 0.0 ± 0.0
Val
8.091ValAla: 8.091 ± 0.747
0.413ValCys: 0.413 ± 0.145
4.725ValAsp: 4.725 ± 0.887
4.311ValGlu: 4.311 ± 0.531
2.067ValPhe: 2.067 ± 0.33
3.839ValGly: 3.839 ± 0.553
1.595ValHis: 1.595 ± 0.351
2.481ValIle: 2.481 ± 0.453
3.189ValLys: 3.189 ± 0.379
5.552ValLeu: 5.552 ± 0.668
2.008ValMet: 2.008 ± 0.298
2.717ValAsn: 2.717 ± 0.45
4.252ValPro: 4.252 ± 0.516
2.599ValGln: 2.599 ± 0.354
4.016ValArg: 4.016 ± 0.498
4.252ValSer: 4.252 ± 0.54
5.138ValThr: 5.138 ± 0.517
4.489ValVal: 4.489 ± 0.586
1.063ValTrp: 1.063 ± 0.236
1.89ValTyr: 1.89 ± 0.33
0.0ValXaa: 0.0 ± 0.0
Trp
2.244TrpAla: 2.244 ± 0.513
0.354TrpCys: 0.354 ± 0.139
1.358TrpAsp: 1.358 ± 0.296
1.181TrpGlu: 1.181 ± 0.286
0.236TrpPhe: 0.236 ± 0.126
1.181TrpGly: 1.181 ± 0.251
0.413TrpHis: 0.413 ± 0.192
0.827TrpIle: 0.827 ± 0.234
0.413TrpLys: 0.413 ± 0.149
1.772TrpLeu: 1.772 ± 0.316
0.295TrpMet: 0.295 ± 0.152
0.827TrpAsn: 0.827 ± 0.267
0.709TrpPro: 0.709 ± 0.248
0.591TrpGln: 0.591 ± 0.194
1.89TrpArg: 1.89 ± 0.353
1.358TrpSer: 1.358 ± 0.274
0.709TrpThr: 0.709 ± 0.192
1.299TrpVal: 1.299 ± 0.277
0.295TrpTrp: 0.295 ± 0.141
0.236TrpTyr: 0.236 ± 0.109
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.658TyrAla: 2.658 ± 0.377
0.413TyrCys: 0.413 ± 0.154
1.358TyrAsp: 1.358 ± 0.25
2.244TyrGlu: 2.244 ± 0.322
0.886TyrPhe: 0.886 ± 0.239
1.772TyrGly: 1.772 ± 0.249
0.591TyrHis: 0.591 ± 0.191
1.181TyrIle: 1.181 ± 0.238
1.24TyrLys: 1.24 ± 0.254
2.008TyrLeu: 2.008 ± 0.409
0.591TyrMet: 0.591 ± 0.168
1.122TyrAsn: 1.122 ± 0.231
1.122TyrPro: 1.122 ± 0.222
1.122TyrGln: 1.122 ± 0.286
2.54TyrArg: 2.54 ± 0.397
1.358TyrSer: 1.358 ± 0.243
2.185TyrThr: 2.185 ± 0.371
2.008TyrVal: 2.008 ± 0.331
0.236TyrTrp: 0.236 ± 0.138
0.472TyrTyr: 0.472 ± 0.18
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 98 proteins (16933 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski