Desulfosporosinus orientis (strain ATCC 19365 / DSM 765 / NCIMB 8382 / VKM B-1628 / Singapore I) (Desulfotomaculum orientis)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Desulfosporosinus; Desulfosporosinus orientis

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5217 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G7WJ14|G7WJ14_DESOD Cell wall-binding protein OS=Desulfosporosinus orientis (strain ATCC 19365 / DSM 765 / NCIMB 8382 / VKM B-1628 / Singapore I) OX=768706 GN=Desor_4309 PE=4 SV=1
MM1 pKa = 7.0MRR3 pKa = 11.84ALYY6 pKa = 10.46AGVSGLQAHH15 pKa = 6.25EE16 pKa = 4.67KK17 pKa = 10.52KK18 pKa = 9.88MDD20 pKa = 3.76VIGNNIANVNTVGYY34 pKa = 8.94KK35 pKa = 9.92ASRR38 pKa = 11.84VTFSDD43 pKa = 4.19LLSQTLSKK51 pKa = 10.14ATAADD56 pKa = 3.62SNRR59 pKa = 11.84GVGGTNAKK67 pKa = 10.28QIGLGVGIGSIDD79 pKa = 3.53MLMTDD84 pKa = 4.67GGTEE88 pKa = 4.12STGNTTDD95 pKa = 4.58LSLEE99 pKa = 4.01GDD101 pKa = 3.66GFFIVRR107 pKa = 11.84NGVTGSYY114 pKa = 9.26MFTRR118 pKa = 11.84SGDD121 pKa = 3.47FSIDD125 pKa = 3.46EE126 pKa = 4.51NGDD129 pKa = 3.75LVTSNGLNVYY139 pKa = 8.88GWSSYY144 pKa = 7.4STNAVGDD151 pKa = 3.93YY152 pKa = 11.17EE153 pKa = 5.12FDD155 pKa = 3.32TDD157 pKa = 5.3SLVEE161 pKa = 4.39PINIYY166 pKa = 10.91GDD168 pKa = 3.83DD169 pKa = 3.67YY170 pKa = 11.78NGNKK174 pKa = 9.97KK175 pKa = 9.42ILKK178 pKa = 9.57AQATEE183 pKa = 3.87NVIFSGNLDD192 pKa = 3.5SSEE195 pKa = 4.07EE196 pKa = 3.95AVEE199 pKa = 6.02DD200 pKa = 3.92IDD202 pKa = 6.59DD203 pKa = 4.2DD204 pKa = 4.3TEE206 pKa = 4.09PQYY209 pKa = 8.4TTSVTVYY216 pKa = 10.37DD217 pKa = 4.04SLGNEE222 pKa = 3.84HH223 pKa = 7.86DD224 pKa = 3.21ITINYY229 pKa = 8.56IKK231 pKa = 10.47SDD233 pKa = 3.63TNTWDD238 pKa = 3.36CYY240 pKa = 7.42VTYY243 pKa = 10.66DD244 pKa = 4.79SGEE247 pKa = 4.03TDD249 pKa = 3.28SDD251 pKa = 4.05GEE253 pKa = 4.45PILTRR258 pKa = 11.84VDD260 pKa = 3.82LGQLQFDD267 pKa = 4.14EE268 pKa = 4.88YY269 pKa = 11.45GDD271 pKa = 3.71IVEE274 pKa = 5.58DD275 pKa = 4.04DD276 pKa = 3.4SSYY279 pKa = 9.72PTTQVLTISPDD290 pKa = 2.88TGAADD295 pKa = 3.75MEE297 pKa = 4.39ITLDD301 pKa = 4.84FSDD304 pKa = 4.79LCTGADD310 pKa = 3.68DD311 pKa = 4.38SSVEE315 pKa = 3.92AEE317 pKa = 4.17EE318 pKa = 4.08VDD320 pKa = 4.41GYY322 pKa = 11.33SAGTLEE328 pKa = 5.91DD329 pKa = 3.57ISIDD333 pKa = 3.76SNGVIMGVYY342 pKa = 9.66TNGAQQPLGMIGIAQFANSSGLQKK366 pKa = 9.76TGSGYY371 pKa = 11.15YY372 pKa = 9.66IATANSGDD380 pKa = 3.48FMNGVSANGAISSGTLEE397 pKa = 4.2MSNVDD402 pKa = 3.48LSYY405 pKa = 10.98EE406 pKa = 4.48FSQMITTQRR415 pKa = 11.84GYY417 pKa = 10.07QANSTLITTADD428 pKa = 3.47EE429 pKa = 4.1MLEE432 pKa = 3.93TLINMKK438 pKa = 10.29RR439 pKa = 3.25

Molecular weight: 46.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G7W504|G7W504_DESOD Uncharacterized protein OS=Desulfosporosinus orientis (strain ATCC 19365 / DSM 765 / NCIMB 8382 / VKM B-1628 / Singapore I) OX=768706 GN=Desor_0150 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 9.19QPKK8 pKa = 8.78NRR10 pKa = 11.84RR11 pKa = 11.84HH12 pKa = 5.44KK13 pKa = 10.09RR14 pKa = 11.84VHH16 pKa = 5.93GFLSRR21 pKa = 11.84MSTPTGRR28 pKa = 11.84NVIKK32 pKa = 10.5RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.52GRR39 pKa = 11.84KK40 pKa = 8.8KK41 pKa = 10.81LSVV44 pKa = 3.15

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5217

0

5217

1624322

22

2868

311.4

34.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.802 ± 0.037

1.19 ± 0.013

4.9 ± 0.027

6.843 ± 0.041

4.1 ± 0.026

7.511 ± 0.037

1.792 ± 0.015

7.568 ± 0.034

6.162 ± 0.027

10.338 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.74 ± 0.018

4.195 ± 0.022

3.923 ± 0.022

3.721 ± 0.027

4.485 ± 0.03

6.133 ± 0.031

5.191 ± 0.029

7.066 ± 0.029

1.046 ± 0.014

3.293 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski