Streptococcus phage Javan23

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4D6A8W1|A0A4D6A8W1_9CAUD Uncharacterized protein OS=Streptococcus phage Javan23 OX=2548057 GN=Javan23_0013 PE=4 SV=1
MM1 pKa = 7.52LFGASNEE8 pKa = 4.02YY9 pKa = 10.49GKK11 pKa = 8.46TVGYY15 pKa = 10.51FDD17 pKa = 5.62LNLVIDD23 pKa = 4.41KK24 pKa = 9.89PQDD27 pKa = 3.26KK28 pKa = 10.74LIYY31 pKa = 9.44CVLMDD36 pKa = 4.09SANLVKK42 pKa = 10.78SLDD45 pKa = 3.59DD46 pKa = 3.72YY47 pKa = 10.82YY48 pKa = 11.33TYY50 pKa = 10.44LTFANKK56 pKa = 9.18EE57 pKa = 3.95

Molecular weight:
6.55 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4D6A902|A0A4D6A902_9CAUD Uncharacterized protein OS=Streptococcus phage Javan23 OX=2548057 GN=Javan23_0053 PE=4 SV=1
MM1 pKa = 7.13SQGISVKK8 pKa = 10.26VDD10 pKa = 2.73VDD12 pKa = 3.79LAGATRR18 pKa = 11.84KK19 pKa = 9.81VSPASVARR27 pKa = 11.84GRR29 pKa = 11.84VAMASQMMMDD39 pKa = 4.02MDD41 pKa = 4.45RR42 pKa = 11.84YY43 pKa = 10.1IPLRR47 pKa = 11.84SGGGALRR54 pKa = 11.84ASASMGGSGKK64 pKa = 8.8TISYY68 pKa = 6.95NTVYY72 pKa = 10.89ARR74 pKa = 11.84AQFYY78 pKa = 7.61GTNGIVTFSKK88 pKa = 9.65YY89 pKa = 5.91TTPGTGKK96 pKa = 10.34DD97 pKa = 2.91WLKK100 pKa = 9.95PAKK103 pKa = 9.44EE104 pKa = 4.22ANLEE108 pKa = 4.02SWKK111 pKa = 10.66QKK113 pKa = 10.31ALKK116 pKa = 10.6EE117 pKa = 4.03MGFF120 pKa = 3.66

Molecular weight:
12.85 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

11642

41

1269

207.9

23.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.769 ± 0.947

0.593 ± 0.097

6.614 ± 0.457

6.365 ± 0.605

4.441 ± 0.211

6.477 ± 0.688

1.331 ± 0.152

7.859 ± 0.286

8.255 ± 0.446

7.774 ± 0.25

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.465 ± 0.175

6.124 ± 0.382

2.671 ± 0.248

4.269 ± 0.228

3.616 ± 0.248

6.76 ± 0.456

6.382 ± 0.348

6.219 ± 0.296

1.151 ± 0.133

3.865 ± 0.404

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski