Magnetovibrio blakemorei

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Thalassospiraceae; Magnetovibrio

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3316 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E5QA13|A0A1E5QA13_9PROT Metal-dependent phosphohydrolase OS=Magnetovibrio blakemorei OX=28181 GN=BEN30_07215 PE=4 SV=1
MM1 pKa = 7.11QAISSEE7 pKa = 4.3KK8 pKa = 8.67VCFVIVKK15 pKa = 10.17SRR17 pKa = 11.84EE18 pKa = 3.42FDD20 pKa = 3.37AKK22 pKa = 10.49EE23 pKa = 3.79DD24 pKa = 3.73APYY27 pKa = 10.69EE28 pKa = 4.3DD29 pKa = 4.49PGGNASDD36 pKa = 5.39DD37 pKa = 3.69GDD39 pKa = 4.04HH40 pKa = 6.88QILSHH45 pKa = 6.47QPDD48 pKa = 3.47DD49 pKa = 3.66SVYY52 pKa = 9.99EE53 pKa = 3.9EE54 pKa = 4.22LVSFFEE60 pKa = 4.29SLSDD64 pKa = 3.68EE65 pKa = 4.47EE66 pKa = 4.94LCEE69 pKa = 3.9LHH71 pKa = 7.53AMVMLGRR78 pKa = 11.84GDD80 pKa = 3.29VDD82 pKa = 3.44MEE84 pKa = 4.2GWDD87 pKa = 3.67EE88 pKa = 4.24AVQTAQDD95 pKa = 4.36DD96 pKa = 4.25LDD98 pKa = 4.4EE99 pKa = 4.63NTPKK103 pKa = 10.55HH104 pKa = 6.07LLEE107 pKa = 5.42IPLISDD113 pKa = 3.71YY114 pKa = 11.03LAEE117 pKa = 4.46ALSQFGCSCSDD128 pKa = 3.11

Molecular weight: 14.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E5QBP1|A0A1E5QBP1_9PROT Urease subunit beta OS=Magnetovibrio blakemorei OX=28181 GN=ureB PE=3 SV=1
MM1 pKa = 6.86MHH3 pKa = 6.76NANHH7 pKa = 4.99STKK10 pKa = 10.45RR11 pKa = 11.84QIAFRR16 pKa = 11.84NGSHH20 pKa = 6.33NRR22 pKa = 11.84PKK24 pKa = 10.52VRR26 pKa = 11.84AKK28 pKa = 10.3AAQAPAFGPVLMVLGFVSLAVLAFASWIQTPALSFF63 pKa = 3.98

Molecular weight: 6.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3316

0

3316

1029642

41

6909

310.5

33.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.515 ± 0.045

0.958 ± 0.017

6.106 ± 0.036

5.823 ± 0.036

3.808 ± 0.029

8.034 ± 0.042

2.344 ± 0.023

5.607 ± 0.039

4.55 ± 0.044

9.958 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.808 ± 0.028

3.308 ± 0.028

4.562 ± 0.035

3.549 ± 0.027

5.587 ± 0.043

5.741 ± 0.042

5.611 ± 0.063

7.531 ± 0.033

1.231 ± 0.016

2.367 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski