Propionibacterium sp. DORA_15

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Propionibacteriaceae; Propionibacterium; unclassified Propionibacterium

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 565 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W1UA60|W1UA60_9ACTN DNA mismatch repair protein (Fragment) OS=Propionibacterium sp. DORA_15 OX=1403938 GN=Q613_PSC00023G0001 PE=4 SV=1
DDD2 pKa = 5.05DD3 pKa = 3.59TYYY6 pKa = 11.5FVDDD10 pKa = 3.01GGLKKK15 pKa = 9.3TFSIDDD21 pKa = 3.09NYYY24 pKa = 10.07LLTLVYYY31 pKa = 10.76DDD33 pKa = 3.21DDD35 pKa = 4.51LALHHH40 pKa = 6.02V

Molecular weight: 4.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W1U0X6|W1U0X6_9ACTN Uncharacterized protein (Fragment) OS=Propionibacterium sp. DORA_15 OX=1403938 GN=Q613_PSC00193G0001 PE=4 SV=1
MM1 pKa = 7.68HH2 pKa = 7.56IGTLVVLLVVCVVVRR17 pKa = 11.84IAMTKK22 pKa = 10.34LKK24 pKa = 10.62AAFARR29 pKa = 11.84DD30 pKa = 3.71HH31 pKa = 6.52LRR33 pKa = 11.84GLAATKK39 pKa = 10.04EE40 pKa = 4.01ISFASAALAVATMVIVPRR58 pKa = 11.84SALVVSHH65 pKa = 6.34VALIAMSTAYY75 pKa = 8.5VTRR78 pKa = 11.84RR79 pKa = 11.84LLQGAEE85 pKa = 4.17RR86 pKa = 11.84QGSTTVRR93 pKa = 11.84TYY95 pKa = 10.86VRR97 pKa = 11.84YY98 pKa = 10.02RR99 pKa = 11.84CDD101 pKa = 3.38RR102 pKa = 11.84TWQEE106 pKa = 4.12TKK108 pKa = 10.78SKK110 pKa = 10.78AKK112 pKa = 10.18RR113 pKa = 11.84ALQDD117 pKa = 3.43PGCC120 pKa = 4.34

Molecular weight: 13.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

565

0

565

79208

20

797

140.2

15.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.793 ± 0.15

1.098 ± 0.052

6.105 ± 0.117

5.497 ± 0.151

3.62 ± 0.078

7.748 ± 0.126

2.328 ± 0.064

5.833 ± 0.123

4.751 ± 0.164

9.267 ± 0.137

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.587 ± 0.064

3.455 ± 0.126

4.544 ± 0.086

3.203 ± 0.084

5.782 ± 0.121

6.786 ± 0.124

5.892 ± 0.102

7.849 ± 0.143

1.497 ± 0.063

2.745 ± 0.089
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski