Curvibacter phage TJ1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6B8K0|A0A5J6B8K0_9CAUD Phage_Mu_F domain-containing protein OS=Curvibacter phage TJ1 OX=2290809 PE=4 SV=1
MM1 pKa = 7.54FDD3 pKa = 2.99VATRR7 pKa = 11.84PQPNTVDD14 pKa = 3.3AASVFGMPFDD24 pKa = 3.67WRR26 pKa = 11.84LVTPGPAVNYY36 pKa = 7.03PWKK39 pKa = 10.89DD40 pKa = 3.46FVQPNGVPVVYY51 pKa = 10.71ADD53 pKa = 3.57VLATYY58 pKa = 10.53SLEE61 pKa = 5.32LEE63 pKa = 4.59DD64 pKa = 4.12TLQTAVILSLFTDD77 pKa = 3.89RR78 pKa = 11.84RR79 pKa = 11.84AGIDD83 pKa = 3.36DD84 pKa = 4.74KK85 pKa = 11.48LPQGEE90 pKa = 4.09TDD92 pKa = 3.0RR93 pKa = 11.84RR94 pKa = 11.84GWVGDD99 pKa = 3.78EE100 pKa = 3.99FTTDD104 pKa = 4.18DD105 pKa = 4.01FDD107 pKa = 5.29SRR109 pKa = 11.84PDD111 pKa = 3.24PWGSLLWTCYY121 pKa = 10.07GGKK124 pKa = 9.75SSQEE128 pKa = 3.63LLEE131 pKa = 4.14RR132 pKa = 11.84ARR134 pKa = 11.84FAAAEE139 pKa = 4.03ALDD142 pKa = 3.21WMVRR146 pKa = 11.84DD147 pKa = 5.45GIASRR152 pKa = 11.84VSVIAQWVGEE162 pKa = 4.21RR163 pKa = 11.84QDD165 pKa = 3.48RR166 pKa = 11.84LAVRR170 pKa = 11.84PSIYY174 pKa = 10.27KK175 pKa = 9.63PGQVTPVYY183 pKa = 10.28DD184 pKa = 4.1VLWGTSVKK192 pKa = 10.52RR193 pKa = 11.84FIQQ196 pKa = 3.47

Molecular weight: 22.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6B7B0|A0A5J6B7B0_9CAUD Thermonuclease-like protein OS=Curvibacter phage TJ1 OX=2290809 PE=4 SV=1
MM1 pKa = 6.83EE2 pKa = 5.89RR3 pKa = 11.84GWIFNKK9 pKa = 9.5GALWIGAHH17 pKa = 5.33YY18 pKa = 11.07SPFNRR23 pKa = 11.84RR24 pKa = 11.84WCINVLPCVTFWTAMPGGKK43 pKa = 10.07APDD46 pKa = 3.28RR47 pKa = 11.84STRR50 pKa = 3.34

Molecular weight: 5.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

11620

50

685

215.2

23.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.504 ± 0.596

0.947 ± 0.103

5.955 ± 0.304

5.396 ± 0.334

3.055 ± 0.176

8.107 ± 0.391

1.67 ± 0.159

4.157 ± 0.169

4.793 ± 0.329

9.036 ± 0.284

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.823 ± 0.17

3.287 ± 0.221

4.501 ± 0.216

4.243 ± 0.181

6.351 ± 0.322

5.74 ± 0.265

5.981 ± 0.409

7.332 ± 0.327

1.583 ± 0.144

2.539 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski