Microbacterium phage Nebulous

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Neferthenavirus; unclassified Neferthenavirus

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M9Z497|A0A6M9Z497_9CAUD DNA helicase OS=Microbacterium phage Nebulous OX=2736270 GN=39 PE=4 SV=1
MM1 pKa = 7.36TAMDD5 pKa = 5.36DD6 pKa = 4.27LNALLAPLPGYY17 pKa = 10.59AVLTEE22 pKa = 3.86NMKK25 pKa = 10.06QHH27 pKa = 6.79ALDD30 pKa = 3.57ISLVPDD36 pKa = 4.22AAGVWPGQPGYY47 pKa = 11.15VEE49 pKa = 4.43TYY51 pKa = 10.0DD52 pKa = 3.96VYY54 pKa = 10.56WAALSLTGYY63 pKa = 10.05MMAQPFVKK71 pKa = 10.18QAASEE76 pKa = 4.48GTSTTVDD83 pKa = 3.28APNWSAITAYY93 pKa = 9.98FRR95 pKa = 11.84SQSPIACATTGAILTEE111 pKa = 4.3VPIPGLPHH119 pKa = 6.39VVPTDD124 pKa = 3.29MSGYY128 pKa = 10.61GGDD131 pKa = 3.81GYY133 pKa = 11.64GDD135 pKa = 3.73VDD137 pKa = 3.56TDD139 pKa = 3.99LAA141 pKa = 5.6

Molecular weight: 14.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M9Z4B2|A0A6M9Z4B2_9CAUD Uncharacterized protein OS=Microbacterium phage Nebulous OX=2736270 GN=30 PE=4 SV=1
MM1 pKa = 7.78RR2 pKa = 11.84NSKK5 pKa = 9.52AQCGHH10 pKa = 5.77FTRR13 pKa = 11.84KK14 pKa = 9.41HH15 pKa = 6.0FYY17 pKa = 9.83RR18 pKa = 11.84VHH20 pKa = 6.35NSLAGRR26 pKa = 11.84VVIMKK31 pKa = 7.72CQWTLCQKK39 pKa = 10.24PLAISHH45 pKa = 7.11DD46 pKa = 3.79ALLEE50 pKa = 4.04LVARR54 pKa = 4.88

Molecular weight: 6.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

13394

47

811

235.0

25.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.52 ± 0.405

0.627 ± 0.087

6.481 ± 0.399

6.07 ± 0.389

2.957 ± 0.182

8.004 ± 0.36

1.755 ± 0.187

4.883 ± 0.385

4.771 ± 0.304

8.34 ± 0.321

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.352 ± 0.197

3.36 ± 0.267

5.361 ± 0.23

4.293 ± 0.228

5.846 ± 0.382

5.256 ± 0.312

6.861 ± 0.415

7.391 ± 0.32

1.986 ± 0.196

2.889 ± 0.198
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski