Amino acid dipepetide frequency for Mycobacterium phage Ariel

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.522AlaAla: 11.522 ± 0.915
1.77AlaCys: 1.77 ± 0.31
6.704AlaAsp: 6.704 ± 0.485
8.155AlaGlu: 8.155 ± 0.686
2.902AlaPhe: 2.902 ± 0.295
7.169AlaGly: 7.169 ± 0.774
2.119AlaHis: 2.119 ± 0.274
5.224AlaIle: 5.224 ± 0.382
4.063AlaLys: 4.063 ± 0.35
8.591AlaLeu: 8.591 ± 0.539
3.221AlaMet: 3.221 ± 0.319
3.047AlaAsn: 3.047 ± 0.372
4.063AlaPro: 4.063 ± 0.379
2.757AlaGln: 2.757 ± 0.308
6.472AlaArg: 6.472 ± 0.499
4.469AlaSer: 4.469 ± 0.395
4.789AlaThr: 4.789 ± 0.359
5.659AlaVal: 5.659 ± 0.427
2.061AlaTrp: 2.061 ± 0.276
2.902AlaTyr: 2.902 ± 0.323
0.0AlaXaa: 0.0 ± 0.0
Cys
1.19CysAla: 1.19 ± 0.239
0.203CysCys: 0.203 ± 0.093
1.393CysAsp: 1.393 ± 0.268
1.045CysGlu: 1.045 ± 0.205
0.464CysPhe: 0.464 ± 0.139
2.206CysGly: 2.206 ± 0.328
0.609CysHis: 0.609 ± 0.153
0.668CysIle: 0.668 ± 0.127
0.726CysLys: 0.726 ± 0.16
1.045CysLeu: 1.045 ± 0.213
0.145CysMet: 0.145 ± 0.074
0.638CysAsn: 0.638 ± 0.133
0.929CysPro: 0.929 ± 0.185
0.377CysGln: 0.377 ± 0.116
0.958CysArg: 0.958 ± 0.25
0.929CysSer: 0.929 ± 0.205
0.842CysThr: 0.842 ± 0.166
0.842CysVal: 0.842 ± 0.165
0.29CysTrp: 0.29 ± 0.091
0.464CysTyr: 0.464 ± 0.128
0.0CysXaa: 0.0 ± 0.0
Asp
5.601AspAla: 5.601 ± 0.498
0.987AspCys: 0.987 ± 0.198
4.411AspAsp: 4.411 ± 0.392
4.905AspGlu: 4.905 ± 0.442
2.148AspPhe: 2.148 ± 0.263
6.124AspGly: 6.124 ± 0.485
1.828AspHis: 1.828 ± 0.254
2.815AspIle: 2.815 ± 0.32
2.931AspLys: 2.931 ± 0.291
5.311AspLeu: 5.311 ± 0.374
1.364AspMet: 1.364 ± 0.231
2.09AspAsn: 2.09 ± 0.235
3.338AspPro: 3.338 ± 0.298
2.235AspGln: 2.235 ± 0.259
3.889AspArg: 3.889 ± 0.328
2.757AspSer: 2.757 ± 0.229
3.454AspThr: 3.454 ± 0.327
4.411AspVal: 4.411 ± 0.411
2.293AspTrp: 2.293 ± 0.252
2.119AspTyr: 2.119 ± 0.238
0.0AspXaa: 0.0 ± 0.0
Glu
6.675GluAla: 6.675 ± 0.497
1.451GluCys: 1.451 ± 0.266
4.528GluAsp: 4.528 ± 0.366
5.775GluGlu: 5.775 ± 0.496
2.989GluPhe: 2.989 ± 0.27
4.411GluGly: 4.411 ± 0.351
1.306GluHis: 1.306 ± 0.226
4.063GluIle: 4.063 ± 0.368
2.844GluLys: 2.844 ± 0.288
6.849GluLeu: 6.849 ± 0.521
2.467GluMet: 2.467 ± 0.278
1.886GluAsn: 1.886 ± 0.244
3.251GluPro: 3.251 ± 0.323
2.351GluGln: 2.351 ± 0.227
4.498GluArg: 4.498 ± 0.455
3.338GluSer: 3.338 ± 0.329
3.28GluThr: 3.28 ± 0.273
4.934GluVal: 4.934 ± 0.435
1.364GluTrp: 1.364 ± 0.193
2.815GluTyr: 2.815 ± 0.367
0.0GluXaa: 0.0 ± 0.0
Phe
2.438PheAla: 2.438 ± 0.281
0.406PheCys: 0.406 ± 0.112
2.583PheAsp: 2.583 ± 0.247
2.177PheGlu: 2.177 ± 0.257
1.132PhePhe: 1.132 ± 0.226
3.192PheGly: 3.192 ± 0.359
0.668PheHis: 0.668 ± 0.137
1.335PheIle: 1.335 ± 0.186
1.016PheLys: 1.016 ± 0.17
2.206PheLeu: 2.206 ± 0.232
0.638PheMet: 0.638 ± 0.145
1.393PheAsn: 1.393 ± 0.208
2.003PhePro: 2.003 ± 0.243
0.813PheGln: 0.813 ± 0.179
1.596PheArg: 1.596 ± 0.242
2.293PheSer: 2.293 ± 0.283
2.032PheThr: 2.032 ± 0.216
2.351PheVal: 2.351 ± 0.303
0.58PheTrp: 0.58 ± 0.138
0.9PheTyr: 0.9 ± 0.187
0.0PheXaa: 0.0 ± 0.0
Gly
7.198GlyAla: 7.198 ± 0.781
1.509GlyCys: 1.509 ± 0.247
5.398GlyAsp: 5.398 ± 0.36
5.979GlyGlu: 5.979 ± 0.417
2.786GlyPhe: 2.786 ± 0.286
9.345GlyGly: 9.345 ± 1.64
2.351GlyHis: 2.351 ± 0.269
3.802GlyIle: 3.802 ± 0.405
3.541GlyLys: 3.541 ± 0.38
6.994GlyLeu: 6.994 ± 0.628
2.061GlyMet: 2.061 ± 0.27
3.047GlyAsn: 3.047 ± 0.357
3.657GlyPro: 3.657 ± 0.362
2.554GlyGln: 2.554 ± 0.322
5.746GlyArg: 5.746 ± 0.35
5.108GlySer: 5.108 ± 0.627
5.05GlyThr: 5.05 ± 0.486
5.659GlyVal: 5.659 ± 0.449
2.119GlyTrp: 2.119 ± 0.278
3.425GlyTyr: 3.425 ± 0.304
0.0GlyXaa: 0.0 ± 0.0
His
2.119HisAla: 2.119 ± 0.234
0.435HisCys: 0.435 ± 0.134
1.596HisAsp: 1.596 ± 0.24
1.857HisGlu: 1.857 ± 0.267
0.638HisPhe: 0.638 ± 0.138
1.974HisGly: 1.974 ± 0.243
0.668HisHis: 0.668 ± 0.159
0.842HisIle: 0.842 ± 0.171
0.726HisLys: 0.726 ± 0.135
2.264HisLeu: 2.264 ± 0.248
0.464HisMet: 0.464 ± 0.126
0.58HisAsn: 0.58 ± 0.138
1.451HisPro: 1.451 ± 0.21
0.9HisGln: 0.9 ± 0.166
2.177HisArg: 2.177 ± 0.256
0.987HisSer: 0.987 ± 0.153
0.842HisThr: 0.842 ± 0.159
1.712HisVal: 1.712 ± 0.225
0.609HisTrp: 0.609 ± 0.149
0.755HisTyr: 0.755 ± 0.163
0.0HisXaa: 0.0 ± 0.0
Ile
5.717IleAla: 5.717 ± 0.436
0.493IleCys: 0.493 ± 0.128
3.541IleAsp: 3.541 ± 0.338
3.947IleGlu: 3.947 ± 0.329
1.219IlePhe: 1.219 ± 0.206
3.57IleGly: 3.57 ± 0.393
1.248IleHis: 1.248 ± 0.205
1.974IleIle: 1.974 ± 0.211
1.741IleLys: 1.741 ± 0.196
3.396IleLeu: 3.396 ± 0.376
0.929IleMet: 0.929 ± 0.174
1.915IleAsn: 1.915 ± 0.241
3.309IlePro: 3.309 ± 0.278
1.654IleGln: 1.654 ± 0.177
2.641IleArg: 2.641 ± 0.309
2.409IleSer: 2.409 ± 0.228
2.931IleThr: 2.931 ± 0.335
3.338IleVal: 3.338 ± 0.358
0.784IleTrp: 0.784 ± 0.174
1.509IleTyr: 1.509 ± 0.197
0.0IleXaa: 0.0 ± 0.0
Lys
4.237LysAla: 4.237 ± 0.344
0.493LysCys: 0.493 ± 0.118
1.741LysAsp: 1.741 ± 0.231
1.828LysGlu: 1.828 ± 0.245
1.364LysPhe: 1.364 ± 0.194
2.873LysGly: 2.873 ± 0.319
1.045LysHis: 1.045 ± 0.191
1.915LysIle: 1.915 ± 0.225
2.264LysLys: 2.264 ± 0.302
3.367LysLeu: 3.367 ± 0.41
1.77LysMet: 1.77 ± 0.209
0.697LysAsn: 0.697 ± 0.134
2.264LysPro: 2.264 ± 0.255
1.161LysGln: 1.161 ± 0.19
2.902LysArg: 2.902 ± 0.402
2.09LysSer: 2.09 ± 0.403
1.857LysThr: 1.857 ± 0.206
3.134LysVal: 3.134 ± 0.269
1.132LysTrp: 1.132 ± 0.201
1.306LysTyr: 1.306 ± 0.186
0.0LysXaa: 0.0 ± 0.0
Leu
8.475LeuAla: 8.475 ± 0.505
1.016LeuCys: 1.016 ± 0.202
5.369LeuAsp: 5.369 ± 0.408
4.847LeuGlu: 4.847 ± 0.441
1.828LeuPhe: 1.828 ± 0.203
6.791LeuGly: 6.791 ± 0.566
1.886LeuHis: 1.886 ± 0.234
3.599LeuIle: 3.599 ± 0.388
2.902LeuLys: 2.902 ± 0.301
5.63LeuLeu: 5.63 ± 0.403
1.974LeuMet: 1.974 ± 0.262
2.67LeuAsn: 2.67 ± 0.3
4.411LeuPro: 4.411 ± 0.385
2.351LeuGln: 2.351 ± 0.257
6.559LeuArg: 6.559 ± 0.418
5.108LeuSer: 5.108 ± 0.444
5.05LeuThr: 5.05 ± 0.372
4.818LeuVal: 4.818 ± 0.365
1.509LeuTrp: 1.509 ± 0.229
1.828LeuTyr: 1.828 ± 0.283
0.0LeuXaa: 0.0 ± 0.0
Met
1.915MetAla: 1.915 ± 0.254
0.145MetCys: 0.145 ± 0.07
1.19MetAsp: 1.19 ± 0.198
1.306MetGlu: 1.306 ± 0.202
0.638MetPhe: 0.638 ± 0.136
2.09MetGly: 2.09 ± 0.278
0.435MetHis: 0.435 ± 0.123
1.103MetIle: 1.103 ± 0.183
1.074MetLys: 1.074 ± 0.185
1.886MetLeu: 1.886 ± 0.272
0.609MetMet: 0.609 ± 0.129
1.48MetAsn: 1.48 ± 0.203
1.277MetPro: 1.277 ± 0.207
0.668MetGln: 0.668 ± 0.156
1.712MetArg: 1.712 ± 0.216
2.67MetSer: 2.67 ± 0.269
2.496MetThr: 2.496 ± 0.3
1.045MetVal: 1.045 ± 0.17
0.522MetTrp: 0.522 ± 0.12
0.638MetTyr: 0.638 ± 0.135
0.0MetXaa: 0.0 ± 0.0
Asn
3.105AsnAla: 3.105 ± 0.358
0.551AsnCys: 0.551 ± 0.138
2.119AsnAsp: 2.119 ± 0.273
1.741AsnGlu: 1.741 ± 0.247
0.958AsnPhe: 0.958 ± 0.162
3.802AsnGly: 3.802 ± 0.397
1.016AsnHis: 1.016 ± 0.18
1.306AsnIle: 1.306 ± 0.189
1.219AsnLys: 1.219 ± 0.177
2.641AsnLeu: 2.641 ± 0.315
0.638AsnMet: 0.638 ± 0.139
0.755AsnAsn: 0.755 ± 0.154
2.351AsnPro: 2.351 ± 0.312
1.016AsnGln: 1.016 ± 0.183
2.409AsnArg: 2.409 ± 0.25
1.596AsnSer: 1.596 ± 0.274
1.683AsnThr: 1.683 ± 0.227
2.206AsnVal: 2.206 ± 0.225
0.697AsnTrp: 0.697 ± 0.122
0.755AsnTyr: 0.755 ± 0.133
0.0AsnXaa: 0.0 ± 0.0
Pro
5.108ProAla: 5.108 ± 0.398
0.958ProCys: 0.958 ± 0.246
3.251ProAsp: 3.251 ± 0.303
4.644ProGlu: 4.644 ± 0.341
1.799ProPhe: 1.799 ± 0.262
5.34ProGly: 5.34 ± 0.622
0.813ProHis: 0.813 ± 0.138
2.496ProIle: 2.496 ± 0.248
1.945ProLys: 1.945 ± 0.284
4.179ProLeu: 4.179 ± 0.33
1.074ProMet: 1.074 ± 0.166
1.915ProAsn: 1.915 ± 0.227
2.728ProPro: 2.728 ± 0.294
1.277ProGln: 1.277 ± 0.247
2.844ProArg: 2.844 ± 0.326
2.467ProSer: 2.467 ± 0.313
3.28ProThr: 3.28 ± 0.335
3.947ProVal: 3.947 ± 0.395
1.248ProTrp: 1.248 ± 0.206
1.335ProTyr: 1.335 ± 0.236
0.0ProXaa: 0.0 ± 0.0
Gln
3.134GlnAla: 3.134 ± 0.297
0.435GlnCys: 0.435 ± 0.135
1.451GlnAsp: 1.451 ± 0.221
2.003GlnGlu: 2.003 ± 0.328
0.987GlnPhe: 0.987 ± 0.182
1.915GlnGly: 1.915 ± 0.258
0.871GlnHis: 0.871 ± 0.172
1.393GlnIle: 1.393 ± 0.201
1.625GlnLys: 1.625 ± 0.251
2.496GlnLeu: 2.496 ± 0.316
1.132GlnMet: 1.132 ± 0.159
0.958GlnAsn: 0.958 ± 0.183
1.77GlnPro: 1.77 ± 0.239
1.103GlnGln: 1.103 ± 0.208
2.206GlnArg: 2.206 ± 0.243
2.032GlnSer: 2.032 ± 0.259
1.596GlnThr: 1.596 ± 0.251
1.886GlnVal: 1.886 ± 0.224
0.58GlnTrp: 0.58 ± 0.14
0.813GlnTyr: 0.813 ± 0.144
0.0GlnXaa: 0.0 ± 0.0
Arg
6.617ArgAla: 6.617 ± 0.631
1.248ArgCys: 1.248 ± 0.205
4.237ArgAsp: 4.237 ± 0.349
5.05ArgGlu: 5.05 ± 0.5
2.467ArgPhe: 2.467 ± 0.319
5.398ArgGly: 5.398 ± 0.408
1.625ArgHis: 1.625 ± 0.201
3.715ArgIle: 3.715 ± 0.362
3.134ArgLys: 3.134 ± 0.33
4.992ArgLeu: 4.992 ± 0.385
2.09ArgMet: 2.09 ± 0.314
1.945ArgAsn: 1.945 ± 0.218
2.844ArgPro: 2.844 ± 0.322
2.322ArgGln: 2.322 ± 0.28
5.137ArgArg: 5.137 ± 0.521
2.612ArgSer: 2.612 ± 0.194
3.192ArgThr: 3.192 ± 0.379
4.586ArgVal: 4.586 ± 0.388
2.293ArgTrp: 2.293 ± 0.268
2.38ArgTyr: 2.38 ± 0.295
0.0ArgXaa: 0.0 ± 0.0
Ser
5.34SerAla: 5.34 ± 0.558
0.871SerCys: 0.871 ± 0.171
3.367SerAsp: 3.367 ± 0.342
3.425SerGlu: 3.425 ± 0.354
1.509SerPhe: 1.509 ± 0.194
5.979SerGly: 5.979 ± 0.552
1.132SerHis: 1.132 ± 0.184
2.612SerIle: 2.612 ± 0.292
1.857SerLys: 1.857 ± 0.237
4.034SerLeu: 4.034 ± 0.328
1.19SerMet: 1.19 ± 0.209
1.828SerAsn: 1.828 ± 0.226
3.105SerPro: 3.105 ± 0.28
1.654SerGln: 1.654 ± 0.278
3.657SerArg: 3.657 ± 0.337
3.454SerSer: 3.454 ± 0.373
2.844SerThr: 2.844 ± 0.277
3.512SerVal: 3.512 ± 0.333
1.393SerTrp: 1.393 ± 0.204
1.422SerTyr: 1.422 ± 0.184
0.0SerXaa: 0.0 ± 0.0
Thr
5.195ThrAla: 5.195 ± 0.394
1.016ThrCys: 1.016 ± 0.187
3.454ThrAsp: 3.454 ± 0.321
3.512ThrGlu: 3.512 ± 0.337
2.293ThrPhe: 2.293 ± 0.257
5.34ThrGly: 5.34 ± 0.499
1.074ThrHis: 1.074 ± 0.162
3.163ThrIle: 3.163 ± 0.326
1.915ThrLys: 1.915 ± 0.257
4.411ThrLeu: 4.411 ± 0.347
0.842ThrMet: 0.842 ± 0.15
1.741ThrAsn: 1.741 ± 0.277
4.034ThrPro: 4.034 ± 0.419
1.335ThrGln: 1.335 ± 0.203
2.931ThrArg: 2.931 ± 0.278
2.38ThrSer: 2.38 ± 0.322
3.134ThrThr: 3.134 ± 0.367
4.905ThrVal: 4.905 ± 0.458
1.248ThrTrp: 1.248 ± 0.212
2.148ThrTyr: 2.148 ± 0.243
0.0ThrXaa: 0.0 ± 0.0
Val
6.646ValAla: 6.646 ± 0.462
0.9ValCys: 0.9 ± 0.169
5.05ValAsp: 5.05 ± 0.411
5.717ValGlu: 5.717 ± 0.471
1.945ValPhe: 1.945 ± 0.225
5.079ValGly: 5.079 ± 0.46
1.48ValHis: 1.48 ± 0.204
4.034ValIle: 4.034 ± 0.368
2.38ValLys: 2.38 ± 0.25
4.092ValLeu: 4.092 ± 0.35
1.422ValMet: 1.422 ± 0.233
2.612ValAsn: 2.612 ± 0.292
3.483ValPro: 3.483 ± 0.32
2.003ValGln: 2.003 ± 0.243
4.557ValArg: 4.557 ± 0.45
4.092ValSer: 4.092 ± 0.326
4.557ValThr: 4.557 ± 0.518
5.456ValVal: 5.456 ± 0.391
0.9ValTrp: 0.9 ± 0.149
2.09ValTyr: 2.09 ± 0.284
0.0ValXaa: 0.0 ± 0.0
Trp
2.032TrpAla: 2.032 ± 0.243
0.551TrpCys: 0.551 ± 0.144
1.277TrpAsp: 1.277 ± 0.163
1.103TrpGlu: 1.103 ± 0.186
0.871TrpPhe: 0.871 ± 0.167
1.451TrpGly: 1.451 ± 0.202
0.755TrpHis: 0.755 ± 0.16
1.016TrpIle: 1.016 ± 0.18
0.435TrpLys: 0.435 ± 0.108
1.799TrpLeu: 1.799 ± 0.268
0.551TrpMet: 0.551 ± 0.147
0.609TrpAsn: 0.609 ± 0.144
0.958TrpPro: 0.958 ± 0.189
0.813TrpGln: 0.813 ± 0.164
2.177TrpArg: 2.177 ± 0.298
1.915TrpSer: 1.915 ± 0.266
1.393TrpThr: 1.393 ± 0.195
1.886TrpVal: 1.886 ± 0.201
0.726TrpTrp: 0.726 ± 0.171
0.697TrpTyr: 0.697 ± 0.143
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.396TyrAla: 3.396 ± 0.333
0.522TyrCys: 0.522 ± 0.141
2.351TyrAsp: 2.351 ± 0.297
2.061TyrGlu: 2.061 ± 0.225
0.813TyrPhe: 0.813 ± 0.149
3.28TyrGly: 3.28 ± 0.338
0.668TyrHis: 0.668 ± 0.125
1.19TyrIle: 1.19 ± 0.187
1.103TyrLys: 1.103 ± 0.177
2.438TyrLeu: 2.438 ± 0.332
0.29TyrMet: 0.29 ± 0.122
0.755TyrAsn: 0.755 ± 0.148
1.422TyrPro: 1.422 ± 0.198
1.045TyrGln: 1.045 ± 0.178
2.815TyrArg: 2.815 ± 0.297
1.48TyrSer: 1.48 ± 0.197
1.77TyrThr: 1.77 ± 0.259
2.206TyrVal: 2.206 ± 0.264
0.668TyrTrp: 0.668 ± 0.144
0.871TyrTyr: 0.871 ± 0.165
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 245 proteins (34457 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski