Escherichia phage vB_EcoM_Schickermooser

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Phapecoctavirus; Escherichia virus Schickermooser

Average proteome isoelectric point is 5.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 284 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482N338|A0A482N338_9CAUD Macro domain-containing protein OS=Escherichia phage vB_EcoM_Schickermooser OX=2508195 GN=Schickermooser_00215 PE=4 SV=1
MM1 pKa = 6.86KK2 pKa = 9.49TLRR5 pKa = 11.84VNIEE9 pKa = 4.18TAWCGCNEE17 pKa = 4.01VVDD20 pKa = 5.13IEE22 pKa = 4.87IEE24 pKa = 3.98DD25 pKa = 4.38DD26 pKa = 3.45MSQEE30 pKa = 4.45DD31 pKa = 4.36IEE33 pKa = 5.02DD34 pKa = 3.93LAHH37 pKa = 6.48EE38 pKa = 4.8TFQDD42 pKa = 3.56YY43 pKa = 10.77CSYY46 pKa = 10.94GFHH49 pKa = 7.38ILEE52 pKa = 4.57EE53 pKa = 5.16KK54 pKa = 10.62EE55 pKa = 4.1DD56 pKa = 3.64AA57 pKa = 4.8

Molecular weight: 6.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482N2E8|A0A482N2E8_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoM_Schickermooser OX=2508195 GN=Schickermooser_00043 PE=4 SV=1
MM1 pKa = 7.27KK2 pKa = 10.18VHH4 pKa = 6.27YY5 pKa = 7.44PHH7 pKa = 7.54PFDD10 pKa = 4.35PKK12 pKa = 9.77KK13 pKa = 10.44KK14 pKa = 10.35AVILRR19 pKa = 11.84QWQRR23 pKa = 11.84VCRR26 pKa = 11.84TKK28 pKa = 11.0CPINSPHH35 pKa = 6.58NVDD38 pKa = 3.89KK39 pKa = 11.02DD40 pKa = 4.09YY41 pKa = 11.52LGHH44 pKa = 7.56LLNTLHH50 pKa = 7.4RR51 pKa = 11.84KK52 pKa = 7.34TSVNNMSRR60 pKa = 11.84NTAA63 pKa = 3.21

Molecular weight: 7.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

284

0

284

45752

31

1017

161.1

18.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.92 ± 0.147

1.473 ± 0.083

6.323 ± 0.126

7.606 ± 0.23

4.426 ± 0.116

6.535 ± 0.195

1.886 ± 0.083

6.288 ± 0.116

6.763 ± 0.205

7.941 ± 0.168

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.848 ± 0.089

5.399 ± 0.132

3.51 ± 0.121

3.104 ± 0.08

4.05 ± 0.124

5.849 ± 0.121

6.011 ± 0.247

7.001 ± 0.164

1.554 ± 0.081

4.513 ± 0.113
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski