Amino acid dipepetide frequency for Synechococcus phage S-H35

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.379AlaAla: 6.379 ± 0.651
0.694AlaCys: 0.694 ± 0.139
4.499AlaAsp: 4.499 ± 0.329
4.454AlaGlu: 4.454 ± 0.336
2.82AlaPhe: 2.82 ± 0.276
6.715AlaGly: 6.715 ± 0.627
0.94AlaHis: 0.94 ± 0.146
3.895AlaIle: 3.895 ± 0.309
4.544AlaLys: 4.544 ± 0.492
5.193AlaLeu: 5.193 ± 0.449
1.5AlaMet: 1.5 ± 0.181
4.074AlaAsn: 4.074 ± 0.413
2.82AlaPro: 2.82 ± 0.29
2.596AlaGln: 2.596 ± 0.285
2.91AlaArg: 2.91 ± 0.285
4.902AlaSer: 4.902 ± 0.445
5.73AlaThr: 5.73 ± 0.53
4.297AlaVal: 4.297 ± 0.335
0.627AlaTrp: 0.627 ± 0.141
2.417AlaTyr: 2.417 ± 0.256
0.0AlaXaa: 0.0 ± 0.0
Cys
0.671CysAla: 0.671 ± 0.119
0.045CysCys: 0.045 ± 0.033
1.007CysAsp: 1.007 ± 0.161
0.739CysGlu: 0.739 ± 0.136
0.627CysPhe: 0.627 ± 0.113
0.716CysGly: 0.716 ± 0.151
0.291CysHis: 0.291 ± 0.081
0.783CysIle: 0.783 ± 0.152
0.627CysLys: 0.627 ± 0.125
0.627CysLeu: 0.627 ± 0.137
0.358CysMet: 0.358 ± 0.104
0.425CysAsn: 0.425 ± 0.101
0.358CysPro: 0.358 ± 0.103
0.313CysGln: 0.313 ± 0.082
0.515CysArg: 0.515 ± 0.118
0.56CysSer: 0.56 ± 0.11
0.425CysThr: 0.425 ± 0.093
0.627CysVal: 0.627 ± 0.135
0.09CysTrp: 0.09 ± 0.045
0.403CysTyr: 0.403 ± 0.105
0.0CysXaa: 0.0 ± 0.0
Asp
5.282AspAla: 5.282 ± 0.464
0.828AspCys: 0.828 ± 0.15
4.678AspAsp: 4.678 ± 0.344
4.23AspGlu: 4.23 ± 0.368
3.469AspPhe: 3.469 ± 0.295
5.775AspGly: 5.775 ± 0.506
0.985AspHis: 0.985 ± 0.17
3.313AspIle: 3.313 ± 0.285
3.536AspLys: 3.536 ± 0.374
5.103AspLeu: 5.103 ± 0.438
1.365AspMet: 1.365 ± 0.171
3.245AspAsn: 3.245 ± 0.295
3.514AspPro: 3.514 ± 0.316
2.193AspGln: 2.193 ± 0.244
2.865AspArg: 2.865 ± 0.22
3.939AspSer: 3.939 ± 0.272
4.096AspThr: 4.096 ± 0.345
4.432AspVal: 4.432 ± 0.351
1.119AspTrp: 1.119 ± 0.216
3.201AspTyr: 3.201 ± 0.26
0.0AspXaa: 0.0 ± 0.0
Glu
3.738GluAla: 3.738 ± 0.338
0.716GluCys: 0.716 ± 0.125
4.409GluAsp: 4.409 ± 0.415
6.133GluGlu: 6.133 ± 0.545
3.492GluPhe: 3.492 ± 0.268
4.118GluGly: 4.118 ± 0.291
1.03GluHis: 1.03 ± 0.163
4.409GluIle: 4.409 ± 0.349
4.476GluLys: 4.476 ± 0.433
4.208GluLeu: 4.208 ± 0.324
2.328GluMet: 2.328 ± 0.325
2.977GluAsn: 2.977 ± 0.271
1.365GluPro: 1.365 ± 0.202
2.305GluGln: 2.305 ± 0.215
2.574GluArg: 2.574 ± 0.283
3.805GluSer: 3.805 ± 0.348
4.275GluThr: 4.275 ± 0.43
4.7GluVal: 4.7 ± 0.321
0.895GluTrp: 0.895 ± 0.147
3.044GluTyr: 3.044 ± 0.24
0.0GluXaa: 0.0 ± 0.0
Phe
2.82PheAla: 2.82 ± 0.262
0.47PheCys: 0.47 ± 0.104
3.559PheAsp: 3.559 ± 0.264
2.619PheGlu: 2.619 ± 0.27
1.858PhePhe: 1.858 ± 0.206
2.574PheGly: 2.574 ± 0.229
0.895PheHis: 0.895 ± 0.182
2.843PheIle: 2.843 ± 0.305
2.529PheLys: 2.529 ± 0.235
2.932PheLeu: 2.932 ± 0.292
1.343PheMet: 1.343 ± 0.194
2.708PheAsn: 2.708 ± 0.225
1.746PhePro: 1.746 ± 0.23
1.41PheGln: 1.41 ± 0.162
1.813PheArg: 1.813 ± 0.185
3.066PheSer: 3.066 ± 0.285
3.223PheThr: 3.223 ± 0.262
2.775PheVal: 2.775 ± 0.28
0.582PheTrp: 0.582 ± 0.118
1.925PheTyr: 1.925 ± 0.197
0.0PheXaa: 0.0 ± 0.0
Gly
5.461GlyAla: 5.461 ± 0.502
0.716GlyCys: 0.716 ± 0.13
4.812GlyAsp: 4.812 ± 0.403
3.917GlyGlu: 3.917 ± 0.293
3.089GlyPhe: 3.089 ± 0.289
7.23GlyGly: 7.23 ± 0.819
1.052GlyHis: 1.052 ± 0.157
3.827GlyIle: 3.827 ± 0.313
3.738GlyLys: 3.738 ± 0.402
4.588GlyLeu: 4.588 ± 0.331
1.903GlyMet: 1.903 ± 0.239
4.365GlyAsn: 4.365 ± 0.412
2.037GlyPro: 2.037 ± 0.296
2.753GlyGln: 2.753 ± 0.265
3.245GlyArg: 3.245 ± 0.319
5.528GlySer: 5.528 ± 0.428
5.976GlyThr: 5.976 ± 0.63
4.969GlyVal: 4.969 ± 0.377
1.142GlyTrp: 1.142 ± 0.154
2.91GlyTyr: 2.91 ± 0.307
0.0GlyXaa: 0.0 ± 0.0
His
0.627HisAla: 0.627 ± 0.135
0.246HisCys: 0.246 ± 0.078
0.94HisAsp: 0.94 ± 0.161
1.276HisGlu: 1.276 ± 0.198
0.895HisPhe: 0.895 ± 0.149
1.052HisGly: 1.052 ± 0.166
0.425HisHis: 0.425 ± 0.173
0.985HisIle: 0.985 ± 0.153
0.918HisLys: 0.918 ± 0.137
1.231HisLeu: 1.231 ± 0.162
0.336HisMet: 0.336 ± 0.108
0.761HisAsn: 0.761 ± 0.13
0.918HisPro: 0.918 ± 0.164
0.537HisGln: 0.537 ± 0.126
0.828HisArg: 0.828 ± 0.152
0.761HisSer: 0.761 ± 0.119
0.895HisThr: 0.895 ± 0.142
1.007HisVal: 1.007 ± 0.188
0.269HisTrp: 0.269 ± 0.079
0.895HisTyr: 0.895 ± 0.151
0.0HisXaa: 0.0 ± 0.0
Ile
4.096IleAla: 4.096 ± 0.33
0.604IleCys: 0.604 ± 0.114
4.767IleAsp: 4.767 ± 0.343
4.365IleGlu: 4.365 ± 0.306
2.507IlePhe: 2.507 ± 0.263
3.805IleGly: 3.805 ± 0.328
0.739IleHis: 0.739 ± 0.145
3.492IleIle: 3.492 ± 0.334
3.626IleLys: 3.626 ± 0.338
4.275IleLeu: 4.275 ± 0.344
0.828IleMet: 0.828 ± 0.12
3.783IleAsn: 3.783 ± 0.288
2.82IlePro: 2.82 ± 0.255
2.552IleGln: 2.552 ± 0.257
2.395IleArg: 2.395 ± 0.224
4.051IleSer: 4.051 ± 0.356
5.081IleThr: 5.081 ± 0.458
3.492IleVal: 3.492 ± 0.332
0.739IleTrp: 0.739 ± 0.168
2.529IleTyr: 2.529 ± 0.249
0.0IleXaa: 0.0 ± 0.0
Lys
3.648LysAla: 3.648 ± 0.382
0.739LysCys: 0.739 ± 0.158
3.827LysAsp: 3.827 ± 0.331
4.275LysGlu: 4.275 ± 0.52
2.686LysPhe: 2.686 ± 0.295
3.29LysGly: 3.29 ± 0.364
1.052LysHis: 1.052 ± 0.193
3.939LysIle: 3.939 ± 0.319
5.305LysLys: 5.305 ± 0.615
4.611LysLeu: 4.611 ± 0.437
1.746LysMet: 1.746 ± 0.296
2.798LysAsn: 2.798 ± 0.305
2.104LysPro: 2.104 ± 0.249
2.619LysGln: 2.619 ± 0.388
2.641LysArg: 2.641 ± 0.288
4.23LysSer: 4.23 ± 0.341
3.536LysThr: 3.536 ± 0.324
4.432LysVal: 4.432 ± 0.344
0.851LysTrp: 0.851 ± 0.159
3.223LysTyr: 3.223 ± 0.297
0.0LysXaa: 0.0 ± 0.0
Leu
5.484LeuAla: 5.484 ± 0.36
0.604LeuCys: 0.604 ± 0.124
4.969LeuAsp: 4.969 ± 0.348
4.745LeuGlu: 4.745 ± 0.336
2.574LeuPhe: 2.574 ± 0.257
4.409LeuGly: 4.409 ± 0.332
1.321LeuHis: 1.321 ± 0.168
4.163LeuIle: 4.163 ± 0.341
4.678LeuLys: 4.678 ± 0.368
5.081LeuLeu: 5.081 ± 0.462
1.276LeuMet: 1.276 ± 0.222
4.611LeuAsn: 4.611 ± 0.353
3.044LeuPro: 3.044 ± 0.296
2.82LeuGln: 2.82 ± 0.229
3.245LeuArg: 3.245 ± 0.283
4.835LeuSer: 4.835 ± 0.376
4.924LeuThr: 4.924 ± 0.386
4.879LeuVal: 4.879 ± 0.324
0.56LeuTrp: 0.56 ± 0.127
3.357LeuTyr: 3.357 ± 0.281
0.0LeuXaa: 0.0 ± 0.0
Met
1.634MetAla: 1.634 ± 0.202
0.313MetCys: 0.313 ± 0.094
1.097MetAsp: 1.097 ± 0.185
1.544MetGlu: 1.544 ± 0.233
0.806MetPhe: 0.806 ± 0.16
1.41MetGly: 1.41 ± 0.22
0.425MetHis: 0.425 ± 0.102
1.365MetIle: 1.365 ± 0.206
1.992MetLys: 1.992 ± 0.294
1.88MetLeu: 1.88 ± 0.208
0.381MetMet: 0.381 ± 0.114
1.209MetAsn: 1.209 ± 0.183
1.209MetPro: 1.209 ± 0.202
1.097MetGln: 1.097 ± 0.148
0.94MetArg: 0.94 ± 0.161
1.835MetSer: 1.835 ± 0.23
1.88MetThr: 1.88 ± 0.194
1.097MetVal: 1.097 ± 0.163
0.336MetTrp: 0.336 ± 0.095
0.694MetTyr: 0.694 ± 0.142
0.0MetXaa: 0.0 ± 0.0
Asn
3.201AsnAla: 3.201 ± 0.327
0.47AsnCys: 0.47 ± 0.089
3.223AsnAsp: 3.223 ± 0.273
3.201AsnGlu: 3.201 ± 0.243
2.552AsnPhe: 2.552 ± 0.259
4.476AsnGly: 4.476 ± 0.455
0.985AsnHis: 0.985 ± 0.132
3.939AsnIle: 3.939 ± 0.333
3.089AsnLys: 3.089 ± 0.327
4.454AsnLeu: 4.454 ± 0.471
1.007AsnMet: 1.007 ± 0.168
3.447AsnAsn: 3.447 ± 0.296
3.089AsnPro: 3.089 ± 0.282
2.238AsnGln: 2.238 ± 0.199
2.35AsnArg: 2.35 ± 0.194
3.648AsnSer: 3.648 ± 0.36
3.939AsnThr: 3.939 ± 0.499
4.051AsnVal: 4.051 ± 0.333
0.604AsnTrp: 0.604 ± 0.1
2.44AsnTyr: 2.44 ± 0.214
0.0AsnXaa: 0.0 ± 0.0
Pro
3.29ProAla: 3.29 ± 0.294
0.448ProCys: 0.448 ± 0.112
2.82ProAsp: 2.82 ± 0.281
2.619ProGlu: 2.619 ± 0.291
1.858ProPhe: 1.858 ± 0.179
3.223ProGly: 3.223 ± 0.332
0.627ProHis: 0.627 ± 0.126
1.947ProIle: 1.947 ± 0.229
2.193ProLys: 2.193 ± 0.296
2.193ProLeu: 2.193 ± 0.276
0.671ProMet: 0.671 ± 0.117
2.328ProAsn: 2.328 ± 0.253
1.701ProPro: 1.701 ± 0.303
1.41ProGln: 1.41 ± 0.175
1.477ProArg: 1.477 ± 0.188
2.865ProSer: 2.865 ± 0.244
2.999ProThr: 2.999 ± 0.286
2.686ProVal: 2.686 ± 0.268
0.627ProTrp: 0.627 ± 0.109
1.903ProTyr: 1.903 ± 0.241
0.0ProXaa: 0.0 ± 0.0
Gln
2.507GlnAla: 2.507 ± 0.262
0.313GlnCys: 0.313 ± 0.103
2.395GlnAsp: 2.395 ± 0.223
2.149GlnGlu: 2.149 ± 0.23
1.522GlnPhe: 1.522 ± 0.171
2.171GlnGly: 2.171 ± 0.254
0.627GlnHis: 0.627 ± 0.119
2.731GlnIle: 2.731 ± 0.249
2.574GlnLys: 2.574 ± 0.312
3.268GlnLeu: 3.268 ± 0.29
0.895GlnMet: 0.895 ± 0.166
1.835GlnAsn: 1.835 ± 0.198
1.209GlnPro: 1.209 ± 0.176
1.388GlnGln: 1.388 ± 0.227
1.746GlnArg: 1.746 ± 0.173
2.216GlnSer: 2.216 ± 0.239
2.238GlnThr: 2.238 ± 0.223
2.596GlnVal: 2.596 ± 0.328
0.448GlnTrp: 0.448 ± 0.117
1.567GlnTyr: 1.567 ± 0.154
0.0GlnXaa: 0.0 ± 0.0
Arg
3.223ArgAla: 3.223 ± 0.356
0.403ArgCys: 0.403 ± 0.106
2.261ArgAsp: 2.261 ± 0.197
2.753ArgGlu: 2.753 ± 0.255
1.791ArgPhe: 1.791 ± 0.224
2.731ArgGly: 2.731 ± 0.259
0.671ArgHis: 0.671 ± 0.14
2.932ArgIle: 2.932 ± 0.321
2.708ArgLys: 2.708 ± 0.298
3.357ArgLeu: 3.357 ± 0.311
1.231ArgMet: 1.231 ± 0.193
2.014ArgAsn: 2.014 ± 0.202
1.365ArgPro: 1.365 ± 0.203
1.791ArgGln: 1.791 ± 0.187
2.283ArgArg: 2.283 ± 0.276
2.171ArgSer: 2.171 ± 0.211
2.417ArgThr: 2.417 ± 0.291
3.648ArgVal: 3.648 ± 0.32
0.47ArgTrp: 0.47 ± 0.118
2.261ArgTyr: 2.261 ± 0.212
0.0ArgXaa: 0.0 ± 0.0
Ser
5.685SerAla: 5.685 ± 0.46
0.604SerCys: 0.604 ± 0.122
4.365SerAsp: 4.365 ± 0.365
3.895SerGlu: 3.895 ± 0.313
3.335SerPhe: 3.335 ± 0.267
5.618SerGly: 5.618 ± 0.482
0.94SerHis: 0.94 ± 0.157
3.76SerIle: 3.76 ± 0.425
3.671SerLys: 3.671 ± 0.304
4.454SerLeu: 4.454 ± 0.312
1.41SerMet: 1.41 ± 0.176
3.693SerAsn: 3.693 ± 0.389
2.462SerPro: 2.462 ± 0.291
2.149SerGln: 2.149 ± 0.225
2.44SerArg: 2.44 ± 0.231
4.566SerSer: 4.566 ± 0.497
4.745SerThr: 4.745 ± 0.444
4.7SerVal: 4.7 ± 0.338
0.694SerTrp: 0.694 ± 0.132
2.664SerTyr: 2.664 ± 0.252
0.0SerXaa: 0.0 ± 0.0
Thr
5.842ThrAla: 5.842 ± 0.521
0.448ThrCys: 0.448 ± 0.099
3.939ThrAsp: 3.939 ± 0.444
4.096ThrGlu: 4.096 ± 0.324
3.245ThrPhe: 3.245 ± 0.294
5.797ThrGly: 5.797 ± 0.613
0.962ThrHis: 0.962 ± 0.134
4.566ThrIle: 4.566 ± 0.362
3.469ThrLys: 3.469 ± 0.34
6.066ThrLeu: 6.066 ± 0.578
1.321ThrMet: 1.321 ± 0.203
3.939ThrAsn: 3.939 ± 0.479
3.402ThrPro: 3.402 ± 0.348
2.417ThrGln: 2.417 ± 0.178
2.507ThrArg: 2.507 ± 0.239
4.678ThrSer: 4.678 ± 0.424
5.551ThrThr: 5.551 ± 0.78
5.417ThrVal: 5.417 ± 0.607
0.716ThrTrp: 0.716 ± 0.111
2.798ThrTyr: 2.798 ± 0.253
0.0ThrXaa: 0.0 ± 0.0
Val
5.081ValAla: 5.081 ± 0.38
0.604ValCys: 0.604 ± 0.113
5.417ValAsp: 5.417 ± 0.479
4.476ValGlu: 4.476 ± 0.314
2.552ValPhe: 2.552 ± 0.232
4.767ValGly: 4.767 ± 0.364
0.739ValHis: 0.739 ± 0.131
4.163ValIle: 4.163 ± 0.367
4.365ValLys: 4.365 ± 0.427
4.118ValLeu: 4.118 ± 0.325
1.679ValMet: 1.679 ± 0.224
4.544ValAsn: 4.544 ± 0.299
2.529ValPro: 2.529 ± 0.271
1.97ValGln: 1.97 ± 0.169
2.82ValArg: 2.82 ± 0.245
4.611ValSer: 4.611 ± 0.365
5.842ValThr: 5.842 ± 0.45
4.723ValVal: 4.723 ± 0.395
0.761ValTrp: 0.761 ± 0.107
2.619ValTyr: 2.619 ± 0.271
0.0ValXaa: 0.0 ± 0.0
Trp
0.873TrpAla: 0.873 ± 0.118
0.179TrpCys: 0.179 ± 0.067
0.783TrpAsp: 0.783 ± 0.122
0.94TrpGlu: 0.94 ± 0.176
0.47TrpPhe: 0.47 ± 0.114
0.671TrpGly: 0.671 ± 0.115
0.381TrpHis: 0.381 ± 0.091
0.694TrpIle: 0.694 ± 0.111
0.851TrpLys: 0.851 ± 0.162
0.783TrpLeu: 0.783 ± 0.156
0.492TrpMet: 0.492 ± 0.112
0.783TrpAsn: 0.783 ± 0.127
0.336TrpPro: 0.336 ± 0.098
0.313TrpGln: 0.313 ± 0.074
0.537TrpArg: 0.537 ± 0.128
0.739TrpSer: 0.739 ± 0.14
0.671TrpThr: 0.671 ± 0.104
0.873TrpVal: 0.873 ± 0.181
0.201TrpTrp: 0.201 ± 0.065
0.649TrpTyr: 0.649 ± 0.118
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.484TyrAla: 2.484 ± 0.202
0.761TyrCys: 0.761 ± 0.136
3.402TyrAsp: 3.402 ± 0.299
2.574TyrGlu: 2.574 ± 0.268
1.544TyrPhe: 1.544 ± 0.217
2.708TyrGly: 2.708 ± 0.265
0.716TyrHis: 0.716 ± 0.144
2.798TyrIle: 2.798 ± 0.298
2.664TyrLys: 2.664 ± 0.266
3.156TyrLeu: 3.156 ± 0.309
1.052TyrMet: 1.052 ± 0.167
2.865TyrAsn: 2.865 ± 0.255
1.858TyrPro: 1.858 ± 0.212
1.522TyrGln: 1.522 ± 0.194
2.35TyrArg: 2.35 ± 0.272
2.843TyrSer: 2.843 ± 0.261
2.731TyrThr: 2.731 ± 0.246
2.977TyrVal: 2.977 ± 0.259
0.492TyrTrp: 0.492 ± 0.115
1.768TyrTyr: 1.768 ± 0.201
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 204 proteins (44679 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski