Tepidibacillus decaturensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Tepidibacillus

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2599 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A135L593|A0A135L593_9BACI Fructose-1 6-bisphosphatase OS=Tepidibacillus decaturensis OX=1413211 GN=glpX PE=3 SV=1
MM1 pKa = 7.31NPMIEE6 pKa = 4.38FCTNNYY12 pKa = 7.95VHH14 pKa = 6.39GTDD17 pKa = 3.96NIKK20 pKa = 10.52QKK22 pKa = 11.27LEE24 pKa = 4.42DD25 pKa = 3.69NPEE28 pKa = 3.95YY29 pKa = 10.94DD30 pKa = 3.66VIEE33 pKa = 4.37YY34 pKa = 10.65NCLGHH39 pKa = 6.63CEE41 pKa = 4.05RR42 pKa = 11.84CAVTPFAAVEE52 pKa = 4.36GEE54 pKa = 4.43FIEE57 pKa = 6.34ADD59 pKa = 4.16DD60 pKa = 5.07PDD62 pKa = 4.81DD63 pKa = 5.85LIDD66 pKa = 5.55LIEE69 pKa = 4.02EE70 pKa = 4.33TIEE73 pKa = 3.69EE74 pKa = 4.14RR75 pKa = 11.84KK76 pKa = 10.59AFDD79 pKa = 5.08DD80 pKa = 6.26LFDD83 pKa = 3.63QLL85 pKa = 4.8

Molecular weight: 9.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A135L7K2|A0A135L7K2_9BACI Aminopeptidase OS=Tepidibacillus decaturensis OX=1413211 GN=U473_00585 PE=3 SV=1
MM1 pKa = 7.6KK2 pKa = 8.69PTFQPNVRR10 pKa = 11.84KK11 pKa = 9.72RR12 pKa = 11.84SKK14 pKa = 9.51VHH16 pKa = 5.89GFRR19 pKa = 11.84KK20 pKa = 10.02RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.91VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2599

0

2599

756038

27

1487

290.9

32.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.444 ± 0.051

0.513 ± 0.014

4.96 ± 0.037

7.398 ± 0.053

4.365 ± 0.042

6.83 ± 0.043

2.108 ± 0.025

8.819 ± 0.05

7.322 ± 0.046

9.913 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.753 ± 0.025

4.418 ± 0.035

3.664 ± 0.031

4.221 ± 0.039

4.003 ± 0.038

5.52 ± 0.033

5.246 ± 0.029

6.927 ± 0.039

1.016 ± 0.02

3.561 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski