Amino acid dipepetide frequency for Vibrio phage X29

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.983AlaAla: 9.983 ± 2.015
0.774AlaCys: 0.774 ± 0.247
4.953AlaAsp: 4.953 ± 0.495
6.268AlaGlu: 6.268 ± 0.596
2.399AlaPhe: 2.399 ± 0.349
5.262AlaGly: 5.262 ± 0.837
0.31AlaHis: 0.31 ± 0.211
4.256AlaIle: 4.256 ± 0.692
6.501AlaLys: 6.501 ± 0.722
6.887AlaLeu: 6.887 ± 0.681
3.018AlaMet: 3.018 ± 0.58
3.869AlaAsn: 3.869 ± 0.556
2.709AlaPro: 2.709 ± 0.44
2.786AlaGln: 2.786 ± 0.8
3.792AlaArg: 3.792 ± 0.511
5.804AlaSer: 5.804 ± 0.843
4.256AlaThr: 4.256 ± 0.735
5.262AlaVal: 5.262 ± 0.65
1.548AlaTrp: 1.548 ± 0.401
1.857AlaTyr: 1.857 ± 0.319
0.0AlaXaa: 0.0 ± 0.0
Cys
1.006CysAla: 1.006 ± 0.258
0.232CysCys: 0.232 ± 0.119
0.774CysAsp: 0.774 ± 0.277
0.696CysGlu: 0.696 ± 0.224
0.696CysPhe: 0.696 ± 0.247
1.083CysGly: 1.083 ± 0.273
0.077CysHis: 0.077 ± 0.064
0.31CysIle: 0.31 ± 0.163
0.929CysLys: 0.929 ± 0.287
0.774CysLeu: 0.774 ± 0.231
0.31CysMet: 0.31 ± 0.155
0.464CysAsn: 0.464 ± 0.172
0.696CysPro: 0.696 ± 0.236
0.696CysGln: 0.696 ± 0.236
0.619CysArg: 0.619 ± 0.241
0.619CysSer: 0.619 ± 0.271
0.464CysThr: 0.464 ± 0.183
0.464CysVal: 0.464 ± 0.197
0.0CysTrp: 0.0 ± 0.0
0.232CysTyr: 0.232 ± 0.108
0.0CysXaa: 0.0 ± 0.0
Asp
3.869AspAla: 3.869 ± 0.527
0.542AspCys: 0.542 ± 0.19
3.947AspAsp: 3.947 ± 0.591
4.566AspGlu: 4.566 ± 0.629
2.631AspPhe: 2.631 ± 0.431
4.488AspGly: 4.488 ± 0.574
1.316AspHis: 1.316 ± 0.306
3.715AspIle: 3.715 ± 0.516
4.024AspLys: 4.024 ± 0.584
6.191AspLeu: 6.191 ± 0.75
1.161AspMet: 1.161 ± 0.352
2.399AspAsn: 2.399 ± 0.463
3.173AspPro: 3.173 ± 0.574
3.328AspGln: 3.328 ± 0.49
2.709AspArg: 2.709 ± 0.525
4.411AspSer: 4.411 ± 0.672
1.625AspThr: 1.625 ± 0.331
4.102AspVal: 4.102 ± 0.632
1.393AspTrp: 1.393 ± 0.37
1.857AspTyr: 1.857 ± 0.39
0.0AspXaa: 0.0 ± 0.0
Glu
5.417GluAla: 5.417 ± 0.833
0.929GluCys: 0.929 ± 0.296
3.173GluAsp: 3.173 ± 0.521
3.715GluGlu: 3.715 ± 0.573
3.482GluPhe: 3.482 ± 0.485
3.869GluGly: 3.869 ± 0.551
0.696GluHis: 0.696 ± 0.221
5.34GluIle: 5.34 ± 0.693
3.792GluLys: 3.792 ± 0.721
6.81GluLeu: 6.81 ± 0.778
2.167GluMet: 2.167 ± 0.429
2.863GluAsn: 2.863 ± 0.43
2.476GluPro: 2.476 ± 0.511
3.715GluGln: 3.715 ± 0.529
3.715GluArg: 3.715 ± 0.539
4.643GluSer: 4.643 ± 0.61
3.25GluThr: 3.25 ± 0.492
4.875GluVal: 4.875 ± 0.712
1.548GluTrp: 1.548 ± 0.407
2.709GluTyr: 2.709 ± 0.372
0.0GluXaa: 0.0 ± 0.0
Phe
2.554PheAla: 2.554 ± 0.367
1.006PheCys: 1.006 ± 0.293
2.863PheAsp: 2.863 ± 0.457
3.018PheGlu: 3.018 ± 0.444
1.161PhePhe: 1.161 ± 0.278
2.863PheGly: 2.863 ± 0.284
0.464PheHis: 0.464 ± 0.215
2.244PheIle: 2.244 ± 0.562
2.863PheLys: 2.863 ± 0.513
2.709PheLeu: 2.709 ± 0.492
1.083PheMet: 1.083 ± 0.264
1.703PheAsn: 1.703 ± 0.395
0.696PhePro: 0.696 ± 0.197
0.851PheGln: 0.851 ± 0.248
1.238PheArg: 1.238 ± 0.37
3.482PheSer: 3.482 ± 0.526
2.167PheThr: 2.167 ± 0.323
2.476PheVal: 2.476 ± 0.5
0.851PheTrp: 0.851 ± 0.227
1.006PheTyr: 1.006 ± 0.268
0.0PheXaa: 0.0 ± 0.0
Gly
5.727GlyAla: 5.727 ± 0.968
0.851GlyCys: 0.851 ± 0.278
4.953GlyAsp: 4.953 ± 0.432
5.108GlyGlu: 5.108 ± 0.589
3.095GlyPhe: 3.095 ± 0.531
4.566GlyGly: 4.566 ± 0.495
0.929GlyHis: 0.929 ± 0.259
3.947GlyIle: 3.947 ± 0.584
4.488GlyLys: 4.488 ± 0.598
4.179GlyLeu: 4.179 ± 0.474
1.238GlyMet: 1.238 ± 0.296
3.25GlyAsn: 3.25 ± 0.493
1.548GlyPro: 1.548 ± 0.424
3.405GlyGln: 3.405 ± 0.698
3.482GlyArg: 3.482 ± 0.545
4.721GlySer: 4.721 ± 0.685
3.56GlyThr: 3.56 ± 0.583
4.643GlyVal: 4.643 ± 0.581
1.161GlyTrp: 1.161 ± 0.246
2.786GlyTyr: 2.786 ± 0.632
0.0GlyXaa: 0.0 ± 0.0
His
0.851HisAla: 0.851 ± 0.298
0.464HisCys: 0.464 ± 0.218
0.387HisAsp: 0.387 ± 0.166
0.696HisGlu: 0.696 ± 0.217
1.161HisPhe: 1.161 ± 0.289
1.393HisGly: 1.393 ± 0.352
0.464HisHis: 0.464 ± 0.192
1.316HisIle: 1.316 ± 0.303
1.006HisLys: 1.006 ± 0.257
0.851HisLeu: 0.851 ± 0.238
0.464HisMet: 0.464 ± 0.183
0.542HisAsn: 0.542 ± 0.218
0.696HisPro: 0.696 ± 0.228
0.464HisGln: 0.464 ± 0.203
0.774HisArg: 0.774 ± 0.286
0.929HisSer: 0.929 ± 0.226
0.774HisThr: 0.774 ± 0.235
1.238HisVal: 1.238 ± 0.298
0.31HisTrp: 0.31 ± 0.157
0.31HisTyr: 0.31 ± 0.149
0.0HisXaa: 0.0 ± 0.0
Ile
5.959IleAla: 5.959 ± 0.706
0.696IleCys: 0.696 ± 0.184
5.03IleAsp: 5.03 ± 0.728
5.804IleGlu: 5.804 ± 0.837
1.238IlePhe: 1.238 ± 0.273
3.947IleGly: 3.947 ± 0.515
1.161IleHis: 1.161 ± 0.277
3.095IleIle: 3.095 ± 0.528
4.179IleLys: 4.179 ± 0.704
4.334IleLeu: 4.334 ± 0.564
1.238IleMet: 1.238 ± 0.346
3.792IleAsn: 3.792 ± 0.491
2.941IlePro: 2.941 ± 0.597
2.863IleGln: 2.863 ± 0.474
3.792IleArg: 3.792 ± 0.636
4.875IleSer: 4.875 ± 0.654
3.482IleThr: 3.482 ± 0.484
2.941IleVal: 2.941 ± 0.542
0.464IleTrp: 0.464 ± 0.194
2.012IleTyr: 2.012 ± 0.419
0.0IleXaa: 0.0 ± 0.0
Lys
5.959LysAla: 5.959 ± 0.751
0.542LysCys: 0.542 ± 0.226
4.024LysAsp: 4.024 ± 0.57
3.792LysGlu: 3.792 ± 0.539
2.554LysPhe: 2.554 ± 0.468
4.488LysGly: 4.488 ± 0.672
1.857LysHis: 1.857 ± 0.359
4.488LysIle: 4.488 ± 0.594
5.262LysLys: 5.262 ± 0.677
5.959LysLeu: 5.959 ± 0.602
1.935LysMet: 1.935 ± 0.393
3.328LysAsn: 3.328 ± 0.495
4.179LysPro: 4.179 ± 0.686
4.179LysGln: 4.179 ± 0.748
4.179LysArg: 4.179 ± 0.565
4.411LysSer: 4.411 ± 0.551
4.024LysThr: 4.024 ± 0.479
5.03LysVal: 5.03 ± 0.531
0.696LysTrp: 0.696 ± 0.248
2.399LysTyr: 2.399 ± 0.341
0.0LysXaa: 0.0 ± 0.0
Leu
7.971LeuAla: 7.971 ± 0.698
1.083LeuCys: 1.083 ± 0.298
5.649LeuAsp: 5.649 ± 0.662
4.875LeuGlu: 4.875 ± 0.558
3.018LeuPhe: 3.018 ± 0.51
5.804LeuGly: 5.804 ± 0.942
0.851LeuHis: 0.851 ± 0.265
5.417LeuIle: 5.417 ± 0.629
6.501LeuLys: 6.501 ± 0.671
6.501LeuLeu: 6.501 ± 0.725
1.935LeuMet: 1.935 ± 0.388
4.024LeuAsn: 4.024 ± 0.458
4.488LeuPro: 4.488 ± 0.744
3.25LeuGln: 3.25 ± 0.501
3.715LeuArg: 3.715 ± 0.518
4.875LeuSer: 4.875 ± 0.94
5.03LeuThr: 5.03 ± 0.542
4.875LeuVal: 4.875 ± 0.6
1.006LeuTrp: 1.006 ± 0.242
1.857LeuTyr: 1.857 ± 0.426
0.0LeuXaa: 0.0 ± 0.0
Met
3.018MetAla: 3.018 ± 0.67
0.31MetCys: 0.31 ± 0.143
1.083MetAsp: 1.083 ± 0.285
0.929MetGlu: 0.929 ± 0.302
1.083MetPhe: 1.083 ± 0.283
2.089MetGly: 2.089 ± 0.465
0.077MetHis: 0.077 ± 0.068
1.703MetIle: 1.703 ± 0.315
1.935MetLys: 1.935 ± 0.363
2.709MetLeu: 2.709 ± 0.411
0.851MetMet: 0.851 ± 0.283
1.083MetAsn: 1.083 ± 0.343
1.548MetPro: 1.548 ± 0.407
0.542MetGln: 0.542 ± 0.249
1.238MetArg: 1.238 ± 0.275
1.47MetSer: 1.47 ± 0.364
1.393MetThr: 1.393 ± 0.325
1.857MetVal: 1.857 ± 0.359
0.155MetTrp: 0.155 ± 0.171
0.542MetTyr: 0.542 ± 0.239
0.0MetXaa: 0.0 ± 0.0
Asn
3.018AsnAla: 3.018 ± 0.531
0.387AsnCys: 0.387 ± 0.167
1.47AsnAsp: 1.47 ± 0.326
2.244AsnGlu: 2.244 ± 0.402
2.167AsnPhe: 2.167 ± 0.491
4.566AsnGly: 4.566 ± 0.579
1.083AsnHis: 1.083 ± 0.332
2.399AsnIle: 2.399 ± 0.367
3.018AsnLys: 3.018 ± 0.446
3.947AsnLeu: 3.947 ± 0.546
1.393AsnMet: 1.393 ± 0.318
2.709AsnAsn: 2.709 ± 0.519
2.244AsnPro: 2.244 ± 0.436
2.399AsnGln: 2.399 ± 0.536
2.476AsnArg: 2.476 ± 0.475
1.857AsnSer: 1.857 ± 0.389
2.941AsnThr: 2.941 ± 0.445
2.554AsnVal: 2.554 ± 0.4
0.851AsnTrp: 0.851 ± 0.259
1.161AsnTyr: 1.161 ± 0.285
0.0AsnXaa: 0.0 ± 0.0
Pro
2.631ProAla: 2.631 ± 0.5
0.232ProCys: 0.232 ± 0.166
3.018ProAsp: 3.018 ± 0.39
3.869ProGlu: 3.869 ± 0.673
1.316ProPhe: 1.316 ± 0.308
2.012ProGly: 2.012 ± 0.367
0.387ProHis: 0.387 ± 0.161
2.709ProIle: 2.709 ± 0.415
3.482ProLys: 3.482 ± 0.622
2.631ProLeu: 2.631 ± 0.376
1.393ProMet: 1.393 ± 0.309
1.703ProAsn: 1.703 ± 0.34
1.316ProPro: 1.316 ± 0.323
1.47ProGln: 1.47 ± 0.528
1.625ProArg: 1.625 ± 0.34
3.56ProSer: 3.56 ± 0.63
2.863ProThr: 2.863 ± 0.37
2.941ProVal: 2.941 ± 0.509
0.619ProTrp: 0.619 ± 0.236
1.316ProTyr: 1.316 ± 0.365
0.0ProXaa: 0.0 ± 0.0
Gln
3.947GlnAla: 3.947 ± 0.883
0.31GlnCys: 0.31 ± 0.154
2.089GlnAsp: 2.089 ± 0.456
2.863GlnGlu: 2.863 ± 0.424
1.161GlnPhe: 1.161 ± 0.296
1.935GlnGly: 1.935 ± 0.378
0.774GlnHis: 0.774 ± 0.249
3.328GlnIle: 3.328 ± 0.516
3.56GlnLys: 3.56 ± 0.566
4.024GlnLeu: 4.024 ± 0.58
1.238GlnMet: 1.238 ± 0.28
1.625GlnAsn: 1.625 ± 0.323
1.625GlnPro: 1.625 ± 0.46
2.554GlnGln: 2.554 ± 0.398
2.786GlnArg: 2.786 ± 0.359
3.405GlnSer: 3.405 ± 0.712
2.089GlnThr: 2.089 ± 0.357
2.631GlnVal: 2.631 ± 0.386
0.929GlnTrp: 0.929 ± 0.273
0.851GlnTyr: 0.851 ± 0.28
0.0GlnXaa: 0.0 ± 0.0
Arg
4.334ArgAla: 4.334 ± 0.698
0.387ArgCys: 0.387 ± 0.188
2.709ArgAsp: 2.709 ± 0.5
3.947ArgGlu: 3.947 ± 0.57
1.703ArgPhe: 1.703 ± 0.316
2.399ArgGly: 2.399 ± 0.424
0.542ArgHis: 0.542 ± 0.154
3.173ArgIle: 3.173 ± 0.573
3.018ArgLys: 3.018 ± 0.529
5.572ArgLeu: 5.572 ± 0.549
1.625ArgMet: 1.625 ± 0.406
1.935ArgAsn: 1.935 ± 0.39
1.78ArgPro: 1.78 ± 0.45
2.012ArgGln: 2.012 ± 0.381
2.399ArgArg: 2.399 ± 0.579
2.631ArgSer: 2.631 ± 0.373
2.941ArgThr: 2.941 ± 0.519
3.715ArgVal: 3.715 ± 0.416
0.387ArgTrp: 0.387 ± 0.177
2.244ArgTyr: 2.244 ± 0.474
0.0ArgXaa: 0.0 ± 0.0
Ser
4.875SerAla: 4.875 ± 0.837
0.542SerCys: 0.542 ± 0.175
4.488SerAsp: 4.488 ± 0.58
5.185SerGlu: 5.185 ± 0.621
2.012SerPhe: 2.012 ± 0.323
5.262SerGly: 5.262 ± 0.674
1.625SerHis: 1.625 ± 0.379
4.953SerIle: 4.953 ± 0.711
5.959SerLys: 5.959 ± 0.722
5.108SerLeu: 5.108 ± 0.662
1.548SerMet: 1.548 ± 0.401
2.941SerAsn: 2.941 ± 0.561
2.089SerPro: 2.089 ± 0.338
2.554SerGln: 2.554 ± 0.598
2.786SerArg: 2.786 ± 0.454
4.875SerSer: 4.875 ± 0.853
3.095SerThr: 3.095 ± 0.413
4.334SerVal: 4.334 ± 0.473
0.851SerTrp: 0.851 ± 0.214
1.857SerTyr: 1.857 ± 0.433
0.0SerXaa: 0.0 ± 0.0
Thr
4.334ThrAla: 4.334 ± 0.814
0.542ThrCys: 0.542 ± 0.204
3.328ThrAsp: 3.328 ± 0.548
3.869ThrGlu: 3.869 ± 0.542
2.476ThrPhe: 2.476 ± 0.52
4.798ThrGly: 4.798 ± 0.599
0.929ThrHis: 0.929 ± 0.365
3.869ThrIle: 3.869 ± 0.457
4.102ThrLys: 4.102 ± 0.632
3.947ThrLeu: 3.947 ± 0.594
1.006ThrMet: 1.006 ± 0.335
1.78ThrAsn: 1.78 ± 0.35
2.863ThrPro: 2.863 ± 0.371
2.089ThrGln: 2.089 ± 0.479
1.857ThrArg: 1.857 ± 0.472
2.709ThrSer: 2.709 ± 0.572
2.554ThrThr: 2.554 ± 0.439
4.179ThrVal: 4.179 ± 0.753
0.696ThrTrp: 0.696 ± 0.191
1.47ThrTyr: 1.47 ± 0.337
0.0ThrXaa: 0.0 ± 0.0
Val
4.798ValAla: 4.798 ± 0.741
0.464ValCys: 0.464 ± 0.228
4.566ValAsp: 4.566 ± 0.578
3.947ValGlu: 3.947 ± 0.62
1.857ValPhe: 1.857 ± 0.375
3.947ValGly: 3.947 ± 0.426
0.696ValHis: 0.696 ± 0.254
4.798ValIle: 4.798 ± 0.728
5.495ValLys: 5.495 ± 0.498
5.03ValLeu: 5.03 ± 0.828
1.316ValMet: 1.316 ± 0.298
3.482ValAsn: 3.482 ± 0.541
2.941ValPro: 2.941 ± 0.456
2.631ValGln: 2.631 ± 0.483
3.328ValArg: 3.328 ± 0.429
4.721ValSer: 4.721 ± 0.608
4.102ValThr: 4.102 ± 0.57
4.643ValVal: 4.643 ± 0.616
0.851ValTrp: 0.851 ± 0.228
2.012ValTyr: 2.012 ± 0.406
0.0ValXaa: 0.0 ± 0.0
Trp
0.774TrpAla: 0.774 ± 0.177
0.232TrpCys: 0.232 ± 0.131
1.161TrpAsp: 1.161 ± 0.267
0.929TrpGlu: 0.929 ± 0.244
0.851TrpPhe: 0.851 ± 0.275
1.393TrpGly: 1.393 ± 0.33
0.31TrpHis: 0.31 ± 0.159
1.083TrpIle: 1.083 ± 0.249
1.083TrpLys: 1.083 ± 0.353
1.78TrpLeu: 1.78 ± 0.36
0.387TrpMet: 0.387 ± 0.169
0.232TrpAsn: 0.232 ± 0.123
0.232TrpPro: 0.232 ± 0.118
0.696TrpGln: 0.696 ± 0.257
1.006TrpArg: 1.006 ± 0.321
1.083TrpSer: 1.083 ± 0.233
0.542TrpThr: 0.542 ± 0.225
1.006TrpVal: 1.006 ± 0.316
0.155TrpTrp: 0.155 ± 0.099
0.387TrpTyr: 0.387 ± 0.186
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.47TyrAla: 1.47 ± 0.334
0.619TyrCys: 0.619 ± 0.184
1.78TyrAsp: 1.78 ± 0.408
2.863TyrGlu: 2.863 ± 0.489
1.238TyrPhe: 1.238 ± 0.255
1.47TyrGly: 1.47 ± 0.289
0.542TyrHis: 0.542 ± 0.241
1.703TyrIle: 1.703 ± 0.389
2.167TyrLys: 2.167 ± 0.378
2.786TyrLeu: 2.786 ± 0.367
0.232TyrMet: 0.232 ± 0.124
1.161TyrAsn: 1.161 ± 0.264
0.851TyrPro: 0.851 ± 0.296
1.238TyrGln: 1.238 ± 0.321
1.935TyrArg: 1.935 ± 0.365
1.857TyrSer: 1.857 ± 0.353
2.244TyrThr: 2.244 ± 0.369
1.857TyrVal: 1.857 ± 0.36
0.851TyrTrp: 0.851 ± 0.254
0.929TyrTyr: 0.929 ± 0.366
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (12923 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski