Actinopolyspora mortivallis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinopolysporales; Actinopolysporaceae; Actinopolyspora

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3817 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T0GV94|A0A2T0GV94_9ACTN Decaprenylphospho-beta-D-erythro-pentofuranosid-2-ulose 2-reductase OS=Actinopolyspora mortivallis OX=33906 GN=CEP50_12570 PE=4 SV=1
MM1 pKa = 7.34FGVTGLLATSTGVAAAFAAADD22 pKa = 4.36DD23 pKa = 4.41GDD25 pKa = 4.16VTEE28 pKa = 5.07KK29 pKa = 10.15ATNSTPPPIVEE40 pKa = 4.57DD41 pKa = 3.71YY42 pKa = 10.75SYY44 pKa = 10.89PGADD48 pKa = 3.54AIEE51 pKa = 4.65AEE53 pKa = 4.41TGIKK57 pKa = 10.16LIEE60 pKa = 4.47GDD62 pKa = 3.78GNIIKK67 pKa = 8.92TGCDD71 pKa = 2.71TDD73 pKa = 3.85TPVIKK78 pKa = 10.88VEE80 pKa = 4.28SVDD83 pKa = 3.71VATSCYY89 pKa = 9.88EE90 pKa = 4.08VIGEE94 pKa = 4.37SGWLTMEE101 pKa = 4.25IPRR104 pKa = 11.84VYY106 pKa = 10.35AIHH109 pKa = 7.12GDD111 pKa = 3.74DD112 pKa = 4.06HH113 pKa = 8.69SVDD116 pKa = 3.4ASLTVNGEE124 pKa = 4.22TEE126 pKa = 4.14NVQISPGEE134 pKa = 3.96YY135 pKa = 8.16TPVGEE140 pKa = 4.55GQQPPDD146 pKa = 3.92NDD148 pKa = 3.46PAALVEE154 pKa = 4.32LRR156 pKa = 11.84VSQQ159 pKa = 3.95

Molecular weight: 16.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T0GTU9|A0A2T0GTU9_9ACTN Peptidase OS=Actinopolyspora mortivallis OX=33906 GN=CEP50_15060 PE=4 SV=1
MM1 pKa = 7.51LALRR5 pKa = 11.84RR6 pKa = 11.84LALRR10 pKa = 11.84RR11 pKa = 11.84LALRR15 pKa = 11.84RR16 pKa = 11.84LGRR19 pKa = 11.84WRR21 pKa = 11.84RR22 pKa = 11.84GLGVALLRR30 pKa = 11.84RR31 pKa = 11.84PLLIVARR38 pKa = 11.84GAGGRR43 pKa = 11.84VRR45 pKa = 11.84LPVRR49 pKa = 11.84VPRR52 pKa = 11.84VPRR55 pKa = 11.84IRR57 pKa = 11.84GSGRR61 pKa = 11.84MLPAFRR67 pKa = 11.84LPPLGGPFRR76 pKa = 11.84TAALGAVLTLVTHH89 pKa = 6.43EE90 pKa = 4.18

Molecular weight: 9.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3817

0

3817

1209028

29

3623

316.7

34.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.52 ± 0.049

0.862 ± 0.012

5.605 ± 0.033

7.09 ± 0.043

2.767 ± 0.022

9.276 ± 0.043

2.449 ± 0.019

2.888 ± 0.026

1.642 ± 0.024

10.388 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.773 ± 0.015

1.998 ± 0.018

5.83 ± 0.033

2.708 ± 0.027

8.833 ± 0.04

5.845 ± 0.028

6.069 ± 0.03

9.028 ± 0.04

1.487 ± 0.018

1.941 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski