Hubei sobemo-like virus 12

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KER3|A0A1L3KER3_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 12 OX=1923197 PE=4 SV=1
MM1 pKa = 7.95DD2 pKa = 4.98FDD4 pKa = 3.64QARR7 pKa = 11.84VQDD10 pKa = 3.38LHH12 pKa = 8.34AVVFEE17 pKa = 5.06RR18 pKa = 11.84IRR20 pKa = 11.84QLEE23 pKa = 4.07AGEE26 pKa = 4.35YY27 pKa = 8.95VCDD30 pKa = 4.68PIKK33 pKa = 11.11VFVKK37 pKa = 10.06QEE39 pKa = 3.48PHH41 pKa = 6.22KK42 pKa = 10.92AKK44 pKa = 10.69KK45 pKa = 8.36LTNEE49 pKa = 3.96KK50 pKa = 10.09YY51 pKa = 10.1RR52 pKa = 11.84LISAVSLVDD61 pKa = 3.22TMIDD65 pKa = 3.59RR66 pKa = 11.84ILGRR70 pKa = 11.84SFFEE74 pKa = 4.95SVLEE78 pKa = 4.19NNLEE82 pKa = 4.15VPSAIGWSPNKK93 pKa = 10.06GGYY96 pKa = 8.79RR97 pKa = 11.84ALRR100 pKa = 11.84AIFPKK105 pKa = 10.36GEE107 pKa = 4.09KK108 pKa = 10.5VFTSDD113 pKa = 4.49KK114 pKa = 11.39SNWDD118 pKa = 2.92WTVQKK123 pKa = 10.4WLICAFFEE131 pKa = 5.05VVNEE135 pKa = 4.19LCYY138 pKa = 10.84TEE140 pKa = 5.89DD141 pKa = 3.5KK142 pKa = 10.8EE143 pKa = 5.78LYY145 pKa = 8.42QRR147 pKa = 11.84WYY149 pKa = 9.01ALLWKK154 pKa = 10.27RR155 pKa = 11.84CEE157 pKa = 4.04LLFNRR162 pKa = 11.84AFFKK166 pKa = 10.98FQDD169 pKa = 3.74GTIVQQKK176 pKa = 10.58LPGLQKK182 pKa = 10.52SGCYY186 pKa = 6.46WTIVLNTVGQDD197 pKa = 3.25ILHH200 pKa = 5.81VAAGGVGRR208 pKa = 11.84ILGLGDD214 pKa = 5.67DD215 pKa = 3.93IAQEE219 pKa = 4.29VEE221 pKa = 4.04VDD223 pKa = 3.35PEE225 pKa = 4.19KK226 pKa = 10.94YY227 pKa = 10.03LEE229 pKa = 4.0RR230 pKa = 11.84VQRR233 pKa = 11.84YY234 pKa = 7.55GCEE237 pKa = 3.84VKK239 pKa = 10.47EE240 pKa = 3.97FSIDD244 pKa = 3.31KK245 pKa = 10.6VFDD248 pKa = 3.63FGGYY252 pKa = 9.38HH253 pKa = 5.85YY254 pKa = 10.06EE255 pKa = 4.35GEE257 pKa = 4.34IPTPVYY263 pKa = 10.04HH264 pKa = 6.69GKK266 pKa = 9.26HH267 pKa = 5.48VYY269 pKa = 10.41QMVHH273 pKa = 7.31AEE275 pKa = 4.15VGDD278 pKa = 3.75VALWDD283 pKa = 4.1SYY285 pKa = 11.2LGNYY289 pKa = 9.66AGDD292 pKa = 3.97EE293 pKa = 4.18EE294 pKa = 4.75WFEE297 pKa = 4.88AIRR300 pKa = 11.84KK301 pKa = 5.57VARR304 pKa = 11.84RR305 pKa = 11.84VTGRR309 pKa = 11.84IPKK312 pKa = 9.43SRR314 pKa = 11.84NYY316 pKa = 10.35YY317 pKa = 9.61LSLWKK322 pKa = 10.75DD323 pKa = 3.37EE324 pKa = 4.23

Molecular weight: 37.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KER3|A0A1L3KER3_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 12 OX=1923197 PE=4 SV=1
MM1 pKa = 7.67GFRR4 pKa = 11.84MDD6 pKa = 3.91NNLITTLHH14 pKa = 6.07VIDD17 pKa = 5.12RR18 pKa = 11.84LCEE21 pKa = 3.82IQIKK25 pKa = 7.13TDD27 pKa = 2.84KK28 pKa = 10.28GAVMVHH34 pKa = 6.11KK35 pKa = 10.82EE36 pKa = 3.91EE37 pKa = 4.15FQEE40 pKa = 4.1LDD42 pKa = 3.54NEE44 pKa = 4.86VVFAPYY50 pKa = 7.99TQNMQVLGLAKK61 pKa = 10.46YY62 pKa = 10.13KK63 pKa = 10.31IATVGMSMFDD73 pKa = 3.33MAVVRR78 pKa = 11.84IVGKK82 pKa = 8.98MPYY85 pKa = 9.96VRR87 pKa = 11.84TSGLLKK93 pKa = 10.31KK94 pKa = 9.7NRR96 pKa = 11.84SMFYY100 pKa = 10.36VSYY103 pKa = 10.5HH104 pKa = 6.03GSTAKK109 pKa = 10.43GFSGAPYY116 pKa = 8.43VQGRR120 pKa = 11.84NIYY123 pKa = 8.58GMHH126 pKa = 6.4MGSTTGTHH134 pKa = 6.38NMGLDD139 pKa = 3.27ATYY142 pKa = 9.62IQAMVRR148 pKa = 11.84GLDD151 pKa = 3.43EE152 pKa = 4.72AYY154 pKa = 9.55PWEE157 pKa = 4.6KK158 pKa = 10.09YY159 pKa = 9.63KK160 pKa = 10.87KK161 pKa = 9.38RR162 pKa = 11.84AARR165 pKa = 11.84MKK167 pKa = 10.88GKK169 pKa = 8.25VTPSGGYY176 pKa = 10.37LEE178 pKa = 4.49THH180 pKa = 7.03DD181 pKa = 3.87GYY183 pKa = 11.77KK184 pKa = 10.16RR185 pKa = 11.84VDD187 pKa = 3.0ASKK190 pKa = 11.35YY191 pKa = 9.96NDD193 pKa = 3.61LVEE196 pKa = 4.25QGIVDD201 pKa = 3.48WGEE204 pKa = 4.25LMDD207 pKa = 5.21KK208 pKa = 10.21MEE210 pKa = 4.45YY211 pKa = 10.09DD212 pKa = 3.4QPEE215 pKa = 4.12ADD217 pKa = 3.91LPSANRR223 pKa = 11.84EE224 pKa = 4.13HH225 pKa = 7.22LEE227 pKa = 4.32HH228 pKa = 7.67DD229 pKa = 5.11DD230 pKa = 4.26SDD232 pKa = 3.99SDD234 pKa = 3.31QDD236 pKa = 3.49FRR238 pKa = 11.84SRR240 pKa = 11.84GLPTMDD246 pKa = 3.72RR247 pKa = 11.84PRR249 pKa = 11.84AVASNCPIAGISTDD263 pKa = 3.29PAEE266 pKa = 4.28VVFYY270 pKa = 10.39PGSQQKK276 pKa = 10.09QGTPILTNQTFQHH289 pKa = 5.7SRR291 pKa = 11.84EE292 pKa = 4.17FTDD295 pKa = 3.15HH296 pKa = 7.04LEE298 pKa = 4.5SILAQRR304 pKa = 11.84NDD306 pKa = 3.57LSPTIRR312 pKa = 11.84RR313 pKa = 11.84SLRR316 pKa = 11.84KK317 pKa = 8.47ATNFLKK323 pKa = 10.57SQAMKK328 pKa = 10.94NSTEE332 pKa = 4.03SSKK335 pKa = 10.33QQ336 pKa = 3.26

Molecular weight: 37.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

660

324

336

330.0

37.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.212 ± 0.238

1.212 ± 0.438

6.515 ± 0.234

6.818 ± 1.038

4.242 ± 0.688

7.424 ± 0.012

2.576 ± 0.284

4.697 ± 0.377

6.818 ± 0.192

7.424 ± 0.623

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.182 ± 1.545

3.636 ± 0.377

3.636 ± 0.377

4.697 ± 0.258

5.909 ± 0.031

5.606 ± 1.092

4.848 ± 1.207

7.879 ± 1.157

1.818 ± 0.869

4.848 ± 0.273
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski