bacterium HR24

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2129 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5YKM3|A0A2H5YKM3_9BACT Benzoylformate decarboxylase OS=bacterium HR24 OX=2035419 GN=mdlC_2 PE=3 SV=1
MM1 pKa = 6.52WRR3 pKa = 11.84SWGRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84AGRR12 pKa = 11.84GALFALLVAALVGLPLLAYY31 pKa = 9.59RR32 pKa = 11.84LAGADD37 pKa = 3.77PPTDD41 pKa = 3.36IACDD45 pKa = 3.34IDD47 pKa = 4.28GVGVSYY53 pKa = 10.98GVGFAQDD60 pKa = 3.72PPPPGFRR67 pKa = 11.84VSQVILTGVASSCAGASASVSLYY90 pKa = 8.66STPPPSGTAIASGTATVPTAFSGGSVPVDD119 pKa = 3.31MSPDD123 pKa = 3.55PLAQDD128 pKa = 3.88VNWVEE133 pKa = 4.14VVLAGGTAPTVPEE146 pKa = 4.2CGGIKK151 pKa = 9.81VDD153 pKa = 3.67RR154 pKa = 11.84TYY156 pKa = 11.3SGSDD160 pKa = 3.4GPDD163 pKa = 3.21TITGSNFNDD172 pKa = 3.25LMSGNGGDD180 pKa = 3.71DD181 pKa = 3.3QLYY184 pKa = 10.64GGNNKK189 pKa = 9.82DD190 pKa = 4.21CLLGGPGNDD199 pKa = 2.85RR200 pKa = 11.84LYY202 pKa = 11.33GEE204 pKa = 4.85NGNDD208 pKa = 3.25VLLGGGGNDD217 pKa = 3.33SLYY220 pKa = 11.03GGNGDD225 pKa = 3.7DD226 pKa = 4.19VLYY229 pKa = 10.69GGPGTDD235 pKa = 4.08YY236 pKa = 11.47LDD238 pKa = 4.01GGPGRR243 pKa = 11.84DD244 pKa = 2.74ICYY247 pKa = 8.85VQSGEE252 pKa = 4.33TYY254 pKa = 9.71VNCEE258 pKa = 3.9VVRR261 pKa = 11.84PDD263 pKa = 3.36PTAGGSS269 pKa = 3.23

Molecular weight: 27.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5YH74|A0A2H5YH74_9BACT AsnC_trans_reg domain-containing protein OS=bacterium HR24 OX=2035419 GN=HRbin24_00840 PE=4 SV=1
MM1 pKa = 8.03PKK3 pKa = 8.99RR4 pKa = 11.84TYY6 pKa = 9.29QPKK9 pKa = 9.37NIRR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 9.35RR15 pKa = 11.84KK16 pKa = 8.59HH17 pKa = 5.76GFLARR22 pKa = 11.84MATRR26 pKa = 11.84GGRR29 pKa = 11.84LVLKK33 pKa = 10.38RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84LKK38 pKa = 10.74GRR40 pKa = 11.84WRR42 pKa = 11.84LTVV45 pKa = 3.0

Molecular weight: 5.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2129

0

2129

605099

29

2083

284.2

31.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.995 ± 0.075

0.998 ± 0.019

4.823 ± 0.04

6.891 ± 0.065

3.049 ± 0.032

8.993 ± 0.062

2.097 ± 0.028

3.68 ± 0.041

1.92 ± 0.034

11.387 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.143 ± 0.02

1.701 ± 0.029

6.203 ± 0.044

3.442 ± 0.037

9.219 ± 0.058

4.381 ± 0.033

4.199 ± 0.039

8.781 ± 0.049

1.541 ± 0.027

2.556 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski